(Circular) (Six-base) MAPSORT of: pFN21AE6616.seq Check: 6847 from: 1 to: 5710 pFN21AE6616 5710 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3053 1937 Size: 637 479 4594 Fragments arranged by size: 4594 637 479 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2039 3773 5157 5425 1632 Size: 401 6 1734 1384 268 1917 Fragments arranged by size: 1917 1734 1384 401 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2025 4609 5586 1203 Size: 822 2584 977 1327 Fragments arranged by size: 2584 1327 977 822 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2302 3453 5025 2302 Size: 1151 1572 2987 Fragments arranged by size: 2987 1572 1151 ApaI G_GGCC'C Cuts at: 2690 2690 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2216 2832 2910 3035 1870 Size: 73 273 616 78 125 4545 Fragments arranged by size: 4545 616 273 125 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2276 2309 2906 2939 2276 Size: 33 597 33 5047 Fragments arranged by size: 5047 597 33 33 BamHI G'GATC_C Cuts at: 2872 3040 3407 2872 Size: 168 367 5175 Fragments arranged by size: 5175 367 168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2311 3031 4486 618 Size: 325 217 627 524 720 1455 1842 Fragments arranged by size: 1842 1455 720 627 524 325 217 BanII G_rGCy'C Cuts at: 1391 2690 2739 2990 3029 1391 Size: 1299 49 251 39 4072 Fragments arranged by size: 4072 1299 251 49 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2846 928 Size: 971 947 3792 Fragments arranged by size: 3792 971 947 BbvCI CC'TCA_GC Cuts at: 2346 2346 Size: 5710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2659 2743 4321 Size: 107 122 71 631 605 987 84 1578 Cuts at: 4321 136 Size: 1525 Fragments arranged by size: 1578 1525 987 631 605 122 107 84 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2316 2690 3771 4927 1790 Size: 526 374 1081 1156 2573 Fragments arranged by size: 2573 1156 1081 526 374 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 2906 4370 1239 Size: 1625 42 1464 2579 Fragments arranged by size: 2579 1625 1464 42 Bpu10I CC'TnA_GC Cuts at: 2346 2346 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2371 4373 882 Size: 617 872 2002 2219 Fragments arranged by size: 2219 2002 872 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2384 2633 2975 1186 Size: 868 330 249 342 3921 Fragments arranged by size: 3921 868 342 330 249 BseYI C'CCAG_C Cuts at: 2160 2202 2430 2457 2738 2995 4913 5409 2160 Size: 42 228 27 281 257 1918 496 2461 Fragments arranged by size: 2461 1918 496 281 257 228 42 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2890 1163 Size: 573 1154 3983 Fragments arranged by size: 3983 1154 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 2707 3160 3921 4949 1540 Size: 275 186 54 652 453 761 1028 2301 Fragments arranged by size: 2301 1028 761 652 453 275 186 54 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2690 2739 2990 3029 3771 3856 Size: 399 526 374 49 251 39 742 85 Cuts at: 3856 4927 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 374 251 85 49 39 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 844 Size: 2221 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2828 3597 1394 Size: 429 1005 769 3507 Fragments arranged by size: 3507 1005 769 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 4354 4541 1700 Size: 110 247 2297 187 2869 Fragments arranged by size: 2869 2297 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 794 Size: 1535 4175 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2362 2884 2362 Size: 522 5188 BstYI r'GATC_y Cuts at: 1972 2146 2872 3040 3407 3792 3809 5261 5347 Size: 174 726 168 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 726 385 367 346 174 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2691 2691 Size: 5710 BtgI C'CryG_G Cuts at: 513 1065 2470 2635 2775 2891 513 Size: 552 1405 165 140 116 3332 Fragments arranged by size: 3332 1405 552 165 140 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2988 3023 529 Size: 35 2424 35 3216 Fragments arranged by size: 3216 2424 35 35 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DraIII CAC_nnn'GTG Cuts at: 2410 2869 2410 Size: 459 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2183 2704 Size: 54 1425 50 275 18 256 97 521 Cuts at: 2704 3157 4046 5675 8 Size: 453 889 1629 43 Fragments arranged by size: 1629 1425 889 521 453 275 256 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2704 3157 1537 Size: 275 892 453 4090 Fragments arranged by size: 4090 892 453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 3640 1353 Size: 1049 1238 3423 Fragments arranged by size: 3423 1238 1049 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2039 2864 2906 3773 4370 5157 Size: 393 401 6 825 42 867 597 787 Cuts at: 5157 5425 1239 Size: 268 1524 Fragments arranged by size: 1524 867 825 787 597 401 393 268 42 6 EcoNI CCTnn'n_nnAGG Cuts at: 2257 2257 Size: 5710 EcoO109I rG'GnC_Cy Cuts at: 2221 2351 2494 2542 2559 2686 2687 2745 2881 Size: 130 143 48 17 127 1 58 136 Cuts at: 2881 2221 Size: 5050 Fragments arranged by size: 5050 143 136 130 127 58 48 17 1 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 4857 1678 Size: 3179 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373 Size: 32 135 32 2779 32 42 32 2626 Fragments arranged by size: 2779 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3000 3309 3000 Size: 309 5401 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2203 4824 5008 1305 Size: 898 2621 184 2007 Fragments arranged by size: 2621 2007 898 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 3668 4027 4186 518 Size: 648 1303 1199 359 159 2042 Fragments arranged by size: 2042 1303 1199 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2362 2434 2485 2968 2974 3126 3803 Size: 558 198 72 51 483 6 152 677 Cuts at: 3803 4951 5196 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 483 245 198 152 72 51 6 NaeI GCC'GGC Cuts at: 2059 2059 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5158 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2057 2057 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2869 1108 Size: 1761 3949 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2351 2542 2559 2881 2351 Size: 191 17 322 5180 Fragments arranged by size: 5180 322 191 17 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2686 2686 Size: 5710 PstI C_TGCA'G Cuts at: 830 2973 3062 830 Size: 2143 89 3478 Fragments arranged by size: 3478 2143 89 PvuI CG_AT'CG Cuts at: 2001 2055 2001 Size: 54 5656 PvuII CAG'CTG Cuts at: 2164 2968 2164 Size: 804 4906 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2351 2559 2351 Size: 208 5502 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SexAI A'CCwGG_T Cuts at: 2406 2406 Size: 5710 SfcI C'TryA_G Cuts at: 826 1045 2599 2969 3058 4193 4874 5065 826 Size: 219 1554 370 89 1135 681 191 1471 Fragments arranged by size: 1554 1471 1135 681 370 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 2834 3037 1872 Size: 962 203 4545 Fragments arranged by size: 4545 962 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2563 2877 3149 513 Size: 552 1498 314 272 3074 Fragments arranged by size: 3074 1498 552 314 272 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3737 3754 3907 4092 4537 325 Size: 1255 939 1218 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1218 939 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2623 2655 2760 2792 5670 5702 2623 Size: 32 105 32 2878 32 2631 Fragments arranged by size: 2878 2631 105 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2832 3035 1870 Size: 962 203 4545 Fragments arranged by size: 4545 962 203 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI