(Linear) (Six-base) MAPSORT of: KIEE6616.seq Check: 4156 from: 1 to: 1005 KIEE6616.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AccI GT'mk_AC Cuts at: 0 569 1005 Size: 569 436 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 34 1005 Size: 28 6 971 Fragments arranged by size: 971 28 6 AflIII A'CryG_T Cuts at: 0 20 1005 Size: 20 985 AlwNI CAG_nnn'CTG Cuts at: 0 297 1005 Size: 297 708 ApaI G_GGCC'C Cuts at: 0 685 1005 Size: 685 320 AvaI C'yCGr_G Cuts at: 0 211 827 905 1005 Size: 211 616 78 100 Fragments arranged by size: 616 211 100 78 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 271 304 901 934 1005 Size: 271 33 597 33 71 Fragments arranged by size: 597 271 71 33 33 BamHI G'GATC_C Cuts at: 0 867 1005 Size: 867 138 BanI G'GyrC_C Cuts at: 0 306 1005 Size: 306 699 BanII G_rGCy'C Cuts at: 0 685 734 985 1005 Size: 685 49 251 20 Fragments arranged by size: 685 251 49 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 841 1005 Size: 841 164 BbvCI CC'TCA_GC Cuts at: 0 341 1005 Size: 341 664 BglI GCCn_nnn'nGGC Cuts at: 0 654 738 1005 Size: 654 84 267 Fragments arranged by size: 654 267 84 Bme1580I G_kGCm'C Cuts at: 0 311 685 1005 Size: 311 374 320 Fragments arranged by size: 374 320 311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 859 901 1005 Size: 859 42 104 Fragments arranged by size: 859 104 42 Bpu10I CC'TnA_GC Cuts at: 0 341 1005 Size: 341 664 BsaI GGTCTCn'nnnn_ Cuts at: 0 366 1005 Size: 366 639 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 379 628 970 1005 Size: 49 330 249 342 35 Fragments arranged by size: 342 330 249 49 35 BseYI C'CCAG_C Cuts at: 0 155 197 425 452 733 990 1005 Size: 155 42 228 27 281 257 15 Fragments arranged by size: 281 257 228 155 42 27 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 885 1005 Size: 885 120 BsiEI CG_ry'CG Cuts at: 0 50 702 1005 Size: 50 652 303 Fragments arranged by size: 652 303 50 Bsp1286I G_dGCh'C Cuts at: 0 311 685 734 985 1005 Size: 311 374 49 251 20 Fragments arranged by size: 374 311 251 49 20 BsrBI CCG'CTC Cuts at: 0 823 1005 Size: 823 182 BsrFI r'CCGG_y Cuts at: 0 52 1005 Size: 52 953 BstAPI GCAn_nnn'nTGC Cuts at: 0 324 1005 Size: 324 681 BstXI CCAn_nnnn'nTGG Cuts at: 0 357 879 1005 Size: 357 522 126 Fragments arranged by size: 522 357 126 BstYI r'GATC_y Cuts at: 0 141 867 1005 Size: 141 726 138 Fragments arranged by size: 726 141 138 Bsu36I CC'TnA_GG Cuts at: 0 686 1005 Size: 686 319 BtgI C'CryG_G Cuts at: 0 465 630 770 886 1005 Size: 465 165 140 116 119 Fragments arranged by size: 465 165 140 119 116 DraIII CAC_nnn'GTG Cuts at: 0 405 864 1005 Size: 405 459 141 Fragments arranged by size: 459 405 141 EaeI y'GGCC_r Cuts at: 0 81 178 699 1005 Size: 81 97 521 306 Fragments arranged by size: 521 306 97 81 EagI C'GGCC_G Cuts at: 0 699 1005 Size: 699 306 EarI CTCTTCn'nnn_ Cuts at: 0 397 1005 Size: 397 608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 34 859 901 1005 Size: 28 6 825 42 104 Fragments arranged by size: 825 104 42 28 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 252 1005 Size: 252 753 EcoO109I rG'GnC_Cy Cuts at: 0 216 346 489 537 554 681 682 740 Size: 216 130 143 48 17 127 1 58 Cuts at: 740 876 1005 Size: 136 129 Fragments arranged by size: 216 143 136 130 129 127 58 48 17 1 MfeI C'AATT_G Cuts at: 0 995 1005 Size: 995 10 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 198 1005 Size: 198 807 MslI CAynn'nnrTG Cuts at: 0 464 1005 Size: 464 541 MspA1I CmG'CkG Cuts at: 0 159 357 429 480 963 969 1005 Size: 159 198 72 51 483 6 36 Fragments arranged by size: 483 198 159 72 51 36 6 NaeI GCC'GGC Cuts at: 0 54 1005 Size: 54 951 NgoMIV G'CCGG_C Cuts at: 0 52 1005 Size: 52 953 PflMI CCAn_nnn'nTGG Cuts at: 0 864 1005 Size: 864 141 PpuMI rG'GwC_Cy Cuts at: 0 346 537 554 876 1005 Size: 346 191 17 322 129 Fragments arranged by size: 346 322 191 129 17 PspOMI G'GGCC_C Cuts at: 0 681 1005 Size: 681 324 PstI C_TGCA'G Cuts at: 0 968 1005 Size: 968 37 PvuI CG_AT'CG Cuts at: 0 50 1005 Size: 50 955 PvuII CAG'CTG Cuts at: 0 159 963 1005 Size: 159 804 42 Fragments arranged by size: 804 159 42 SanDI GG'GwC_CC Cuts at: 0 346 554 1005 Size: 346 208 451 Fragments arranged by size: 451 346 208 SexAI A'CCwGG_T Cuts at: 0 401 1005 Size: 401 604 SfcI C'TryA_G Cuts at: 0 594 964 1005 Size: 594 370 41 Fragments arranged by size: 594 370 41 SmaI CCC'GGG Cuts at: 0 829 1005 Size: 829 176 StyI C'CwwG_G Cuts at: 0 558 872 1005 Size: 558 314 133 Fragments arranged by size: 558 314 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 514 1005 Size: 514 491 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 618 650 755 787 1005 Size: 618 32 105 32 218 Fragments arranged by size: 618 218 105 32 32 XmaI C'CCGG_G Cuts at: 0 827 1005 Size: 827 178 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI AvaI BaeI BamHI BanI BanII BbsI BbvCI BglI Bme1580I BpmI Bpu10I BsaI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I MfeI MmeI MslI MspA1I NaeI NgoMIV PflMI PpuMI PspOMI PstI PvuI PvuII SanDI SexAI SfcI SmaI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI