################################## # NO CUT ENZYMES ( 209 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccIII 156 TCCGGA T^CCGGA AciI 232 GCGG CCGC(-3/-1) AcsI 142 AAATTT [GA]^AATT[CT] AlwI 198 GGATC GGATC(4/5) ApoI 142 AAATTT [GA]^AATT[CT] BsaWI 156 TCCGGA [AT]^CCGG[AT] BseAI 156 TCCGGA T^CCGGA BsiYI 225 CCTCATTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 225 CCTCATTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 17 GTCTC GTCTC(1/5) BsmFI 207 GTCCC GGGAC(10/14) BspEI 156 TCCGGA T^CCGGA BsrDI 228 CATTGC GCAATG(2/0) Cac8I 33 GCTGGC GC[ACGT]^[ACGT]GC Cfr13I 36 GGCCC G^G[ACGT]CC DdeI 86 CTGAG C^T[ACGT]AG DpnII 199 GATC ^GATC EarI 186 GAAGAG CTCTTC(1/4) HapII 157 CCGG C^CGG HpaII 157 CCGG C^CGG HphI 243 GGTGA GGTGA(8/7) Ksp632I 186 GAAGAG CTCTTC(1/4) MaeIII 28 GTCAC ^GT[ACGT]AC MboI 199 GATC ^GATC MroI 156 TCCGGA T^CCGGA MseI 130 TTAA T^TAA MspI 157 CCGG C^CGG NdeII 199 GATC ^GATC Sau3AI 199 GATC ^GATC Sau96I 36 GGCCC G^G[ACGT]CC SspI 249 AATATT AAT^ATT Tru9I 130 TTAA T^TAA Tsp45I 28 GTCAC ^GT[GC]AC Tsp509I 143 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 169 GCAGC GCAGC(8/12) 44 GCTGC BssKI 51 CCAGG ^CC[ACGT]GG 240 CCTGG BstNI 51 CCAGG CC^[AT]GG 240 CCTGG EcoRII 51 CCAGG ^CC[AT]GG 240 CCTGG Fnu4HI 44 GCTGC GC^[ACGT]GC 169 GCAGC HaeIII 36 GGCC GG^CC 54 GGCC ItaI 44 GCTGC GC^[ACGT]GC 169 GCAGC MvaI 51 CCAGG CC^[AT]GG 240 CCTGG MwoI 24 GCTGGTCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 163 GCTGTTGCAGC ScrFI 51 CCAGG CC^[ACGT]GG 240 CCTGG SfaNI 148 GATGC GCATC(5/9) 191 GATGC TseI 44 GCTGC G^C[AT]GC 169 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AluI MboII MnlI