################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 9 GTCTAC GT^[AC][GT]AC AfaI 477 GTAC GT^AC AlwNI 451 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 200 GAGCCC G[GA]GC[CT]^C BfaI 335 CTAG C^TAG BglI 156 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 468 AGATCT A^GATCT BsaBI 141 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 160 CCATGG C^C[ACGT][ACGT]GG BsmI 248 GCATTC GAATGC(1/-1) BstAPI 450 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 468 AGATCT [GA]^GATC[CT] DpnII 469 GATC ^GATC DsaI 160 CCATGG C^C[GA][CT]GG EcoT14I 160 CCATGG C^C[AT][AT]GG FauI 203 CCCGC CCCGC(4/6) HgaI 95 GACGC GACGC(5/10) HphI 109 GGTGA GGTGA(8/7) MaeI 335 CTAG C^TAG MaeII 495 ACGT A^CGT MamI 141 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 469 GATC ^GATC MboII 252 TCTTC GAAGA(8/7) MflI 468 AGATCT [GA]^GATC[CT] MseI 326 TTAA T^TAA MspA1I 301 CAGCTG C[AC]G^C[GT]G NcoI 160 CCATGG C^CATGG NdeII 469 GATC ^GATC PflFI 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 280 CTGCAG CTGCA^G PvuII 301 CAGCTG CAG^CTG RsaI 477 GTAC GT^AC Sau3AI 469 GATC ^GATC SfaNI 36 GATGC GCATC(5/9) SmlI 472 CTTGAG C^T[CT][GA]AG SspI 390 AATATT AAT^ATT StyI 160 CCATGG C^C[AT][AT]GG TaqI 22 TCGA T^CGA Tru9I 326 TTAA T^TAA Tth111I 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 22 TCGA T^CGA XhoII 468 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 302 AGCT AG^CT 372 AGCT BbvI 282 GCAGC GCAGC(8/12) 300 GCAGC BmyI 200 GAGCCC G[AGT]GC[ACT]^C 461 GTGCCC Bsp1286I 200 GAGCCC G[AGT]GC[ACT]^C 461 GTGCCC BsrI 344 ACTGG ACTGG(1/-1) 359 ACTGG Cfr13I 362 GGGCC G^G[ACGT]CC 411 GGCCC Fnu4HI 282 GCAGC GC^[ACGT]GC 300 GCAGC HaeIII 363 GGCC GG^CC 411 GGCC HinfI 124 GATTC G^A[ACGT]TC 181 GAATC ItaI 282 GCAGC GC^[ACGT]GC 300 GCAGC MaeIII 82 GTGAC ^GT[ACGT]AC 110 GTGAC Sau96I 362 GGGCC G^G[ACGT]CC 411 GGCCC TfiI 124 GATTC G^A[AT]TC 181 GAATC TseI 282 GCAGC G^C[AT]GC 300 GCAGC Tsp45I 82 GTGAC ^GT[GC]AC 110 GTGAC TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^ 78 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AciI BsmAI Cac8I DdeI MnlI MwoI NlaIII SfcI