Restriction map for
pFN21AB7165
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BsrI BssHII Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MflI MluI MroI MseI MunI
NarI NcoI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI ScaI SexAI SfoI SfuI
SnaBI SpeI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AvaII 381 GGACC G^G[AT]CC
BalI 893 TGGCCA TGG^CCA
BfaI 472 CTAG C^TAG
BspLU11I 341 ACATGT A^CATGT
BstEII 195 GGTCACC G^GT[ACGT]ACC
BstPI 195 GGTCACC G^GT[ACGT]ACC
BstXI 371 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 660 GATC ^GATC
EarI 160 CTCTTC CTCTTC(1/4)
EcoNI 798 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 195 GGTCACC G^GT[ACGT]ACC
HinfI 152 GAGTC G^A[ACGT]TC
Ksp632I 160 CTCTTC CTCTTC(1/4)
MaeI 472 CTAG C^TAG
MboI 660 GATC ^GATC
MluNI 893 TGGCCA TGG^CCA
MscI 893 TGGCCA TGG^CCA
MslI 201 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 713 GCCGGC GCC^GGC
NdeII 660 GATC ^GATC
NgoMIV 713 GCCGGC G^CCGGC
PflMI 186 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 152 GAGTC GAGTC(4/5)
PshAI 189 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1125 CAGCTG CAG^CTG
Sau3AI 660 GATC ^GATC
SbfI 390 CCTGCAGG CCTGCA^GG
SfiI 747 GGCCGTCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 712 CGCCGGCG C[GA]^CCGG[CT]G
SphI 179 GCATGC GCATG^C
Sse8387I 390 CCTGCAGG CCTGCA^GG
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 186 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 515 CCATCTCGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 341 ACATGT A^C[GA][CT]GT
788 ACGTGT
BbrPI 591 CACGTG CAC^GTG
787 CACGTG
BglI 748 GCCGTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1042 GCCCGCTCGGC
BlpI 363 GCTCAGC GC^T[ACGT]AGC
1167 GCTGAGC
Bpu1102I 363 GCTCAGC GC^T[ACGT]AGC
1167 GCTGAGC
BsmAI 56 GTCTC GTCTC(1/5)
1103 GTCTC
BsmBI 55 CGTCTC CGTCTC(1/5)
1102 CGTCTC
BsmFI 509 GGGAC GGGAC(10/14)
1137 GTCCC
BspMI 919 ACCTGC ACCTGC(4/8)
393 GCAGGT
BsrBI 838 CCGCTC CCGCTC(-3/-3)
1044 CCGCTC
BsrFI 405 GCCGGT [GA]^CCGG[CT]
713 GCCGGC
BssSI 285 CTCGTG CACGAG(-5/-1)
841 CTCGTG
BstAPI 393 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
536 GCATTATGTGC
CelII 363 GCTCAGC GC^T[ACGT]AGC
1167 GCTGAGC
Cfr10I 405 GCCGGT [GA]^CCGG[CT]
713 GCCGGC
DdeI 364 CTCAG C^T[ACGT]AG
1168 CTGAG
HgaI 1101 GCGTC GACGC(5/10)
1135 GCGTC
MaeIII 196 GTCAC ^GT[ACGT]AC
865 GTCAC
NotI 707 GCGGCCGC GC^GGCCGC
834 GCGGCCGC
PaeR7I 669 CTCGAG C^TCGAG
1179 CTCGAG
PmaCI 591 CACGTG CAC^GTG
787 CACGTG
PmlI 591 CACGTG CAC^GTG
787 CACGTG
PstI 391 CTGCAG CTGCA^G
1068 CTGCAG
SfaNI 586 GATGC GCATC(5/9)
80 GCATC
SfcI 391 CTGCAG C^T[GA][CT]AG
1068 CTGCAG
SmaI 570 CCCGGG CCC^GGG
825 CCCGGG
SmlI 669 CTCGAG C^T[CT][GA]AG
1179 CTCGAG
Tsp45I 196 GTCAC ^GT[GC]AC
865 GTCAC
XhoI 669 CTCGAG C^TCGAG
1179 CTCGAG
XmaCI 570 CCCGGG C^CCGGG
825 CCCGGG
XmaI 570 CCCGGG C^CCGGG
825 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI AviII BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI SacII Sau96I
ScrFI TaqI TseI TthHB8I