(Circular) (Six-base) MAPSORT of: pFN21AB7165.seq Check: 3516 from: 1 to: 5890 pFN21AB7165 5890 bp With 182 enzymes: SgfI * March 28, 2012 12:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2389 2933 2389 Size: 544 5346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 1937 Size: 1296 4594 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3953 5337 5605 1632 Size: 413 1908 1384 268 1917 Fragments arranged by size: 1917 1908 1384 413 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 2346 2793 4789 5766 1203 Size: 1143 447 1996 977 1327 Fragments arranged by size: 1996 1327 1143 977 447 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 2210 1166 Size: 1044 4846 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2654 1184 Size: 1470 4420 AlwNI CAG_nnn'CTG Cuts at: 3633 5205 3633 Size: 1572 4318 ApaI G_GGCC'C Cuts at: 2787 3101 3158 2787 Size: 314 57 5519 Fragments arranged by size: 5519 314 57 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 3199 3425 3577 5686 1493 Size: 1706 226 152 2109 1697 Fragments arranged by size: 2109 1706 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 2575 2674 2830 3184 3215 1870 Size: 73 632 99 156 354 31 4545 Fragments arranged by size: 4545 632 354 156 99 73 31 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2494 2653 3211 4666 Size: 325 217 627 516 191 159 558 1455 Cuts at: 4666 618 Size: 1842 Fragments arranged by size: 1842 1455 627 558 516 325 217 191 159 BanII G_rGCy'C Cuts at: 1391 2787 3101 3158 3209 1391 Size: 1396 314 57 51 4072 Fragments arranged by size: 4072 1396 314 57 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2759 3053 Size: 107 122 71 631 605 337 750 294 Cuts at: 3053 4501 136 Size: 1448 1525 Fragments arranged by size: 1525 1448 750 631 605 337 294 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 2369 3173 3307 2369 Size: 804 134 4952 Fragments arranged by size: 4952 804 134 Bme1580I G_kGCm'C Cuts at: 1790 2306 2476 2499 2787 3101 3158 3951 5107 Size: 516 170 23 288 314 57 793 1156 Cuts at: 5107 1790 Size: 2573 Fragments arranged by size: 2573 1156 793 516 314 288 170 57 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2180 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2066 2828 2993 4550 1239 Size: 809 18 762 165 1557 2579 Fragments arranged by size: 2579 1557 809 762 165 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4015 4880 5178 4015 Size: 865 298 4727 Fragments arranged by size: 4727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4553 882 Size: 617 3054 2219 Fragments arranged by size: 3054 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2598 2794 2917 3681 493 Size: 711 1394 196 123 764 2702 Fragments arranged by size: 2702 1394 764 711 196 123 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5665 Fragments arranged by size: 5665 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2317 3028 3101 1186 Size: 1131 711 73 3975 Fragments arranged by size: 3975 1131 711 73 BseYI C'CCAG_C Cuts at: 2137 2299 2778 3001 3124 5093 5589 2137 Size: 162 479 223 123 1969 496 2438 Fragments arranged by size: 2438 1969 496 479 223 162 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 2201 2306 1163 Size: 573 366 99 105 4747 Fragments arranged by size: 4747 573 366 105 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2109 2411 2688 2716 2754 2843 Size: 275 186 108 302 277 28 38 89 Cuts at: 2843 3340 4101 5129 1540 Size: 497 761 1028 2301 Fragments arranged by size: 2301 1028 761 497 302 277 275 186 108 89 38 28 BsiHKAI G_wGCw'C Cuts at: 2487 2913 2922 3951 4036 5107 2487 Size: 426 9 1029 85 1071 3270 Fragments arranged by size: 3270 1071 1029 426 85 9 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 3113 2066 Size: 1047 4843 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2306 2476 2487 2499 2787 2913 2922 Size: 399 516 170 11 12 288 126 9 Cuts at: 2922 3101 3158 3209 3951 4036 5107 1391 Size: 179 57 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 399 288 179 170 126 85 57 51 12 11 9 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2389 2933 3245 844 Size: 1545 544 312 3489 Fragments arranged by size: 3489 1545 544 312 BsrBI CCG'CTC Cuts at: 1394 1823 2845 3051 3777 1394 Size: 429 1022 206 726 3507 Fragments arranged by size: 3507 1022 726 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2410 2718 4534 4721 1700 Size: 110 600 308 1816 187 2869 Fragments arranged by size: 2869 1816 600 308 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 2290 2846 3950 4962 2290 Size: 556 1104 1012 3218 Fragments arranged by size: 3218 1104 1012 556 BstAPI GCAn_nnn'nTGC Cuts at: 794 2404 2547 794 Size: 1610 143 4137 Fragments arranged by size: 4137 1610 143 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 2200 1184 Size: 1016 4874 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2383 Size: 5890 BstYI r'GATC_y Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885 Size: 1248 367 385 17 1452 86 12 346 Cuts at: 5885 1972 Size: 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2196 2280 2436 2442 2710 3092 513 Size: 552 1131 84 156 6 268 382 3311 Fragments arranged by size: 3311 1131 552 382 268 156 84 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2604 2653 487 Size: 683 56 1378 49 3724 Fragments arranged by size: 3724 1378 683 56 49 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2106 2277 Size: 54 1425 50 275 18 180 96 171 Cuts at: 2277 2408 2439 2622 2685 2713 2723 2751 2760 Size: 131 31 183 63 28 10 28 9 Cuts at: 2760 2840 2898 3337 4226 5855 8 Size: 80 58 439 889 1629 43 Fragments arranged by size: 1629 1425 889 439 275 183 180 171 131 96 80 63 58 54 50 43 31 28 28 18 10 9 EagI C'GGCC_G Cuts at: 1537 1812 2106 2408 2685 2713 2751 2840 3337 Size: 275 294 302 277 28 38 89 497 Cuts at: 3337 1537 Size: 4090 Fragments arranged by size: 4090 497 302 294 277 275 89 38 28 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3820 1353 Size: 818 1649 3423 Fragments arranged by size: 3423 1649 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2048 2066 2828 2993 3953 4550 Size: 393 413 3 18 762 165 960 597 Cuts at: 4550 5337 5605 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 787 762 597 413 393 268 165 18 3 EcoNI CCTnn'n_nnAGG Cuts at: 2807 2807 Size: 5890 EcoO109I rG'GnC_Cy Cuts at: 2579 2783 2784 3097 3098 3155 2579 Size: 204 1 313 1 57 5314 Fragments arranged by size: 5314 313 204 57 1 1 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 2942 2963 3136 4396 2942 Size: 21 173 1260 4436 Fragments arranged by size: 4436 1260 173 21 HaeII r_GCGC'y Cuts at: 1678 2228 2376 2956 3173 5037 1678 Size: 550 148 580 217 1864 2531 Fragments arranged by size: 2531 1864 580 550 217 148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2507 2539 4531 Size: 32 135 32 472 32 431 32 1992 Cuts at: 4531 4563 4605 4637 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1992 472 431 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 5004 5188 1305 Size: 951 2748 184 2007 Fragments arranged by size: 2748 2007 951 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2900 10 Size: 54 1425 343 1068 3000 Fragments arranged by size: 3000 1425 1068 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 3848 4207 4366 518 Size: 648 1044 1638 359 159 2042 Fragments arranged by size: 2042 1638 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2358 2391 2444 2659 2712 3094 3132 3306 Size: 752 33 53 215 53 382 38 174 Cuts at: 3306 3983 5131 5376 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 382 245 215 174 53 53 38 33 NaeI GCC'GGC Cuts at: 2720 2720 Size: 5890 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5338 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NgoMIV G'CCGG_C Cuts at: 2718 2718 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 2713 2840 3337 2713 Size: 127 497 5266 Fragments arranged by size: 5266 497 127 NspI r_CATG'y Cuts at: 1132 1229 2188 2350 2938 3248 4793 1132 Size: 97 959 162 588 310 1545 2229 Fragments arranged by size: 2229 1545 959 588 310 162 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 2346 4789 2346 Size: 2443 3447 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 1108 Size: 1089 4801 PfoI T'CCnGG_A Cuts at: 2043 2511 2043 Size: 468 5422 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PmlI CAC'GTG Cuts at: 2598 2794 2598 Size: 196 5694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2198 1613 Size: 585 5305 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspOMI G'GGCC_C Cuts at: 2783 3097 3154 2783 Size: 314 57 5519 Fragments arranged by size: 5519 314 57 PspXI vC'TCGA_Gb Cuts at: 2674 3184 2674 Size: 510 5380 PstI C_TGCA'G Cuts at: 830 2400 3077 3242 830 Size: 1570 677 165 3478 Fragments arranged by size: 3478 1570 677 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 3132 3132 Size: 5890 SacII CC_GC'GG Cuts at: 2445 2713 3095 2445 Size: 268 382 5240 Fragments arranged by size: 5240 382 268 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SbfI CC_TGCA'GG Cuts at: 2400 3242 2400 Size: 842 5048 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2396 3073 3238 4373 5054 5245 826 Size: 219 1351 677 165 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 677 219 191 165 SfiI GGCCn_nnn'nGGCC Cuts at: 2759 2759 Size: 5890 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 2718 1700 Size: 1018 4872 SmaI CCC'GGG Cuts at: 1872 2577 2832 3217 1872 Size: 705 255 385 4545 Fragments arranged by size: 4545 705 385 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 2674 3184 3994 4895 5157 820 Size: 197 926 731 510 810 901 262 1553 Fragments arranged by size: 1553 926 901 810 731 510 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 2188 3248 1132 Size: 1056 1060 3774 Fragments arranged by size: 3774 1060 1056 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 3329 513 Size: 552 2264 3074 Fragments arranged by size: 3074 2264 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2200 2993 3917 3934 4087 4272 4717 Size: 1255 620 793 924 17 153 185 445 Cuts at: 4717 325 Size: 1498 Fragments arranged by size: 1498 1255 924 793 620 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4136 5848 96 Size: 274 80 33 51 1443 2159 1712 138 Fragments arranged by size: 2159 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2527 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 2674 3184 1943 Size: 731 510 4649 Fragments arranged by size: 4649 731 510 XmaI C'CCGG_G Cuts at: 1870 2575 2830 3215 1870 Size: 705 255 385 4545 Fragments arranged by size: 4545 705 385 255 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PsrI RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI