################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TspRI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AlwI 279 GATCC GGATC(4/5) AlwNI 94 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 567 TGGCCA TGG^CCA BbsI 750 GTCTTC GAAGAC(2/6) BciVI 388 GGATAC GTATCC(6/5) BpuAI 750 GTCTTC GAAGAC(2/6) BsaBI 279 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 761 CTCCTC GAGGAG(10/8) BsrI 578 ACTGG ACTGG(1/-1) Cfr13I 581 GGGCC G^G[ACGT]CC DraI 250 TTTAAA TTT^AAA DrdI 741 GACACCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 567 TGGCCA [CT]^GGCC[GA] EcoT14I 449 CCAAGG C^C[AT][AT]GG HgaI 748 GCGTC GACGC(5/10) HincII 188 GTCAAC GT[CT]^[GA]AC HindII 188 GTCAAC GT[CT]^[GA]AC MaeIII 739 GTGAC ^GT[ACGT]AC MamI 279 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 567 TGGCCA TGG^CCA MscI 567 TGGCCA TGG^CCA NheI 181 GCTAGC G^CTAGC NlaIV 78 GGCTCC GG[ACGT]^[ACGT]CC PflMI 282 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau96I 581 GGGCC G^G[ACGT]CC StyI 449 CCAAGG C^C[AT][AT]GG Tsp45I 739 GTGAC ^GT[GC]AC Tsp509I 318 AATT ^AATT Van91I 282 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 282 CCAGATCATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 134 GTGCTC G[AT]GC[AT]^C 143 GTGCTC BcnI 169 CCGGG CC^[GC]GG 193 CCGGG BfaI 182 CTAG C^TAG 271 CTAG BlpI 136 GCTCAGC GC^T[ACGT]AGC 145 GCTCAGC BmyI 134 GTGCTC G[AGT]GC[ACT]^C 143 GTGCTC Bpu1102I 136 GCTCAGC GC^T[ACGT]AGC 145 GCTCAGC BsaJI 449 CCAAGG C^C[ACGT][ACGT]GG 483 CCTGGG BsiHKAI 134 GTGCTC G[AT]GC[AT]^C 143 GTGCTC Bsp1286I 134 GTGCTC G[AGT]GC[ACT]^C 143 GTGCTC BstF5I 8 CATCC GGATG(2/0) 446 CATCC CelII 136 GCTCAGC GC^T[ACGT]AGC 145 GCTCAGC FokI 8 CATCC GGATG(9/13) 446 CATCC HapII 169 CCGG C^CGG 193 CCGG HinfI 173 GAATC G^A[ACGT]TC 528 GATTC HpaII 169 CCGG C^CGG 193 CCGG HphI 276 GGTGA GGTGA(8/7) 471 GGTGA MaeI 182 CTAG C^TAG 271 CTAG MseI 251 TTAA T^TAA 331 TTAA MslI 519 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 612 CACGCTGATG MspI 169 CCGG C^CGG 193 CCGG NciI 169 CCGGG CC^[GC]GG 193 CCGGG NlaIII 25 CATG CATG^ 288 CATG TfiI 173 GAATC G^A[AT]TC 528 GATTC Tru9I 251 TTAA T^TAA 331 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BbvI BsiYI BslI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI MwoI NdeII Sau3AI ScrFI SfaNI TseI