(Circular) (Six-base) MAPSORT of: pFN21AE6611.seq Check: 8297 from: 1 to: 5506 pFN21AE6611 5506 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3316 278 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2745 3569 4953 5221 1632 Size: 423 690 824 1384 268 1917 Fragments arranged by size: 1917 1384 824 690 423 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AlwNI CAG_nnn'CTG Cuts at: 2104 3249 4821 2104 Size: 1145 1572 2789 Fragments arranged by size: 2789 1572 1145 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2815 3041 3193 5302 1493 Size: 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2831 1870 Size: 73 888 4545 Fragments arranged by size: 4545 888 73 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2827 4282 618 Size: 325 217 627 1040 1455 1842 Fragments arranged by size: 1842 1455 1040 627 325 217 BanII G_rGCy'C Cuts at: 1391 2825 1391 Size: 1434 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2748 928 Size: 971 849 3686 Fragments arranged by size: 3686 971 849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3697 3731 1903 Size: 34 1760 34 3678 Fragments arranged by size: 3678 1760 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2387 3398 4608 2387 Size: 1011 1210 3285 Fragments arranged by size: 3285 1210 1011 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4117 136 Size: 107 122 71 631 605 2445 1525 Fragments arranged by size: 2445 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5501 5501 Size: 5506 BlpI GC'TnA_GC Cuts at: 2142 2151 2923 2142 Size: 9 772 4725 Fragments arranged by size: 4725 772 9 Bme1580I G_kGCm'C Cuts at: 1790 3567 4723 1790 Size: 1777 1156 2573 Fragments arranged by size: 2573 1777 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 3237 4195 Size: 416 53 83 399 24 1176 644 958 Cuts at: 4195 442 Size: 1753 Fragments arranged by size: 1753 1176 958 644 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2190 1056 Size: 1134 4372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4166 1239 Size: 2927 2579 Bpu10I CC'TnA_GC Cuts at: 2133 2133 Size: 5506 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4496 4794 3631 Size: 865 298 4343 Fragments arranged by size: 4343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4169 882 Size: 617 2670 2219 Fragments arranged by size: 2670 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3297 493 Size: 711 2093 2702 Fragments arranged by size: 2702 2093 711 BsaBI GATnn'nnATC Cuts at: 2288 5500 2288 Size: 3212 2294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3313 3695 4330 275 Size: 53 83 186 936 1780 382 635 1451 Fragments arranged by size: 1780 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5281 Fragments arranged by size: 5281 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2757 1186 Size: 1571 3935 BseYI C'CCAG_C Cuts at: 4709 5205 4709 Size: 496 5010 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4933 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2956 3717 4745 1540 Size: 275 186 955 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2143 2152 3567 3652 4723 2143 Size: 9 1415 85 1071 2926 Fragments arranged by size: 2926 1415 1071 85 9 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2152 2825 3567 3652 4723 1391 Size: 399 353 9 673 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 399 353 85 9 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2861 844 Size: 2017 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3393 1394 Size: 429 1570 3507 Fragments arranged by size: 3507 1570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4001 4175 65 Size: 1183 2753 174 1396 Fragments arranged by size: 2753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4150 4337 1700 Size: 110 2340 187 2869 Fragments arranged by size: 2869 2340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 3566 4578 3566 Size: 1012 4494 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5506 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501 Size: 864 367 385 17 1452 86 12 346 Cuts at: 5501 1972 Size: 1977 Fragments arranged by size: 1977 1452 864 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4954 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4767 Fragments arranged by size: 4767 683 56 BtsI GCAGTG_nn' Cuts at: 3022 3816 3836 3022 Size: 794 20 4692 Fragments arranged by size: 4692 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2257 2810 2257 Size: 553 4953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2752 4513 809 Size: 507 1436 1761 1802 Fragments arranged by size: 1802 1761 1436 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2572 2953 3842 Size: 54 1425 50 275 18 742 381 889 Cuts at: 3842 5471 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 742 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2953 1537 Size: 275 1141 4090 Fragments arranged by size: 4090 1141 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3436 1353 Size: 2083 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2745 3569 4166 4953 5221 1239 Size: 393 423 690 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 690 597 423 393 268 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 4012 4012 Size: 5506 HaeII r_GCGC'y Cuts at: 1678 4653 1678 Size: 2975 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373 Size: 32 135 32 2575 32 42 32 2626 Fragments arranged by size: 2626 2575 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2195 2850 3096 669 Size: 988 281 257 655 246 3079 Fragments arranged by size: 3079 988 655 281 257 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2278 4620 4804 1305 Size: 973 2342 184 2007 Fragments arranged by size: 2342 2007 973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2574 10 Size: 54 1425 343 742 2942 Fragments arranged by size: 2942 1425 742 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2621 3464 3823 3982 518 Size: 648 1362 93 843 359 159 2042 Fragments arranged by size: 2042 1362 843 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 2922 3599 4747 4992 1606 Size: 1316 677 1148 245 2120 Fragments arranged by size: 2120 1316 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4954 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 2186 1052 Size: 1134 4372 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NspI r_CATG'y Cuts at: 1132 1229 2864 4409 1132 Size: 97 1635 1545 2229 Fragments arranged by size: 2229 1635 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5506 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 2293 1108 Size: 1185 4321 PfoI T'CCnGG_A Cuts at: 2086 2173 2086 Size: 87 5419 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5506 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PstI C_TGCA'G Cuts at: 830 2858 830 Size: 2028 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SbfI CC_TGCA'GG Cuts at: 2858 2858 Size: 5506 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2854 3989 4670 4861 826 Size: 219 1809 1135 681 191 1471 Fragments arranged by size: 1809 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2833 1872 Size: 961 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3610 4511 4773 820 Size: 197 926 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5506 StyI C'CwwG_G Cuts at: 513 1065 2454 2945 513 Size: 552 1389 491 3074 Fragments arranged by size: 3074 1389 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3533 3550 3703 3888 4333 325 Size: 1255 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3752 5464 96 Size: 274 80 33 51 1443 1775 1712 138 Fragments arranged by size: 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5466 5498 5466 Size: 32 5474 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2294 2294 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2831 1870 Size: 961 4545 XmnI GAAnn'nnTTC Cuts at: 3635 3635 Size: 5506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3314 276 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI