(Linear) (Six-base) MAPSORT of: KIEE6611.seq  Check: 8249  from: 1  to: 801



KIEE6611.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     740     801 
   Size:         50     690      61

  Fragments arranged by size: 

                 690      61      50
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     801 
   Size:         99     702
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     743     801 
   Size:        743      58
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     382     801 
   Size:        382     419
 
BlpI GC'TnA_GC

Cuts at:      0     137     146     801 
   Size:        137       9     655

  Fragments arranged by size: 

                 655     137       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     588     801 
   Size:        588     213
 
BmtI G_CTAG'C

Cuts at:      0     185     801 
   Size:        185     616
 
Bpu10I CC'TnA_GC

Cuts at:      0     128     801 
   Size:        128     673
 
BsaBI GATnn'nnATC

Cuts at:      0     283     801 
   Size:        283     518
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     752     801 
   Size:        752      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     138     147     801 
   Size:        138       9     654

  Fragments arranged by size: 

                 654     138       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     147     801 
   Size:        138       9     654

  Fragments arranged by size: 

                 654     138       9
 
DraI TTT'AAA

Cuts at:      0     252     801 
   Size:        252     549
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     747     801 
   Size:        747      54
 
EaeI y'GGCC_r

Cuts at:      0     567     801 
   Size:        567     234
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     740     801 
   Size:         50     690      61

  Fragments arranged by size: 

                 690      61      50
 
HincII GTy'rAC

Cuts at:      0     190     801 
   Size:        190     611
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     273     801 
   Size:        273     528
 
MscI TGG'CCA

Cuts at:      0     569     801 
   Size:        569     232
 
MslI CAynn'nnrTG

Cuts at:      0     523     616     801 
   Size:        523      93     185

  Fragments arranged by size: 

                 523     185      93
 
NheI G'CTAG_C

Cuts at:      0     181     801 
   Size:        181     620
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288     801 
   Size:        288     513
 
PfoI T'CCnGG_A

Cuts at:      0      81     168     801 
   Size:         81      87     633

  Fragments arranged by size: 

                 633      87      81
 
StyI C'CwwG_G

Cuts at:      0     449     801 
   Size:        449     352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     289     801 
   Size:        289     512
 

 Enzymes that do cut:

     AcuI    AlwNI     BbsI    BciVI     BlpI     BmrI     BmtI   Bpu10I
    BsaBI    BseRI  BsiHKAI Bsp1286I     DraI     DrdI     EaeI  Eco57MI
   HincII     MmeI     MscI     MslI     NheI    PflMI     PfoI     StyI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI