################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 119 GTCTAC GT^[AC][GT]AC AluI 402 AGCT AG^CT BcnI 222 CCCGG CC^[GC]GG BfaI 400 CTAG C^TAG BglI 343 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 25 GAGACC GGTCTC(1/5) BsmI 170 GCATTC GAATGC(1/-1) BsrDI 245 CATTGC GCAATG(2/0) BsrI 344 CCAGT ACTGG(1/-1) DdeI 175 CTGAG C^T[ACGT]AG DsaI 320 CCATGG C^C[GA][CT]GG EcoT14I 320 CCATGG C^C[AT][AT]GG HapII 223 CCGG C^CGG HincII 217 GTCAAC GT[CT]^[GA]AC HindII 217 GTCAAC GT[CT]^[GA]AC HpaII 223 CCGG C^CGG HphI 310 TCACC GGTGA(8/7) MaeI 400 CTAG C^TAG MaeII 117 ACGT A^CGT MnlI 242 CCTC CCTC(7/6) MseI 232 TTAA T^TAA MspI 223 CCGG C^CGG NciI 222 CCCGG CC^[GC]GG NcoI 320 CCATGG C^CATGG NlaIII 321 CATG CATG^ PstI 421 CTGCAG CTGCA^G SfaNI 369 GATGC GCATC(5/9) StyI 320 CCATGG C^C[AT][AT]GG Tru9I 232 TTAA T^TAA XcmI 338 CCAAAGCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AspHI 179 GTGCTC G[AT]GC[AT]^C 406 GTGCTC BmyI 179 GTGCTC G[AGT]GC[ACT]^C 406 GTGCTC BsiHKAI 179 GTGCTC G[AT]GC[AT]^C 406 GTGCTC BsiYI 89 CCAACCAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 93 CCAGGGGCAGG BslI 89 CCAACCAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 93 CCAGGGGCAGG BsmAI 25 GAGAC GTCTC(1/5) 363 GTCTC Bsp1286I 179 GTGCTC G[AGT]GC[ACT]^C 406 GTGCTC MboII 111 GAAGA GAAGA(8/7) 288 TCTTC MwoI 181 GCTCTGTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 343 GCCAGTCTGGC SfcI 121 CTACAG C^T[GA][CT]AG 421 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BbvI BsaJI BssKI BstNI EcoRII Fnu4HI ItaI MslI MvaI ScrFI TseI TspRI