Restriction map for
pFN21AE5236
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
HphI KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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BanII 690 GAGCCC G[GA]GC[CT]^C
BsaAI 448 TACGTG [CT]AC^GT[GA]
BseRI 67 CTCCTC GAGGAG(10/8)
BspLU11I 350 ACATGT A^CATGT
BspMI 287 ACCTGC ACCTGC(4/8)
BstXI 662 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 601 GATC ^GATC
DraII 219 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 445 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 573 CCGCGG C^C[GA][CT]GG
EaeI 468 TGGCCG [CT]^GGCC[GA]
EarI 188 GAAGAG CTCTTC(1/4)
EcoNI 525 CCTTGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 219 GGGACCT [GA]G^G[ACGT]CC[CT]
HindIII 145 AAGCTT A^AGCTT
Ksp632I 188 GAAGAG CTCTTC(1/4)
KspI 573 CCGCGG CCGC^GG
MboI 601 GATC ^GATC
MseI 205 TTAA T^TAA
MspA1I 573 CCGCGG C[AC]G^C[GT]G
NdeII 601 GATC ^GATC
PleI 579 GACTC GAGTC(4/5)
PpuMI 219 GGGACCT [GA]G^G[AT]CC[CT]
SacII 573 CCGCGG CCGC^GG
Sau3AI 601 GATC ^GATC
ScaI 684 AGTACT AGT^ACT
SexAI 530 ACCAGGT A^CC[AT]GGT
TfiI 55 GATTC G^A[AT]TC
Tru9I 205 TTAA T^TAA
XbaI 582 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 19 CGCG CG^CG
574 CGCG
AcsI 201 AAATTT [GA]^AATT[CT]
502 GAATTT
AflIII 350 ACATGT A^C[GA][CT]GT
747 ACGTGT
AluI 146 AGCT AG^CT
234 AGCT
ApoI 201 AAATTT [GA]^AATT[CT]
502 GAATTT
AvaII 220 GGACC G^G[AT]CC
339 GGACC
BanI 42 GGTGCC G^G[CT][GA]CC
165 GGCACC
BbvI 556 GCAGC GCAGC(8/12)
9 GCTGC
BfaI 23 CTAG C^TAG
583 CTAG
BsmFI 219 GGGAC GGGAC(10/14)
577 GGGAC
BsrBI 31 CCGCTC CCGCTC(-3/-3)
330 CCGCTC
BstUI 19 CGCG CG^CG
574 CGCG
DdeI 148 CTTAG C^T[ACGT]AG
688 CTGAG
HaeIII 298 GGCC GG^CC
469 GGCC
HinfI 55 GATTC G^A[ACGT]TC
579 GACTC
MaeI 23 CTAG C^TAG
583 CTAG
MvnI 19 CGCG CG^CG
574 CGCG
NdeI 360 CATATG CA^TATG
695 CATATG
TseI 9 GCTGC G^C[AT]GC
556 GCAGC
Tsp45I 209 GTGAC ^GT[GC]AC
451 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI HhaI HinP1I ItaI MaeII
MaeIII MboII MnlI MvaI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI Tsp509I TspRI