(Circular) (Six-base) MAPSORT of: pFN21AE5236.seq Check: 435 from: 1 to: 5458 pFN21AE5236 5458 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 3521 4905 5173 1632 Size: 624 1265 1384 268 1917 Fragments arranged by size: 1917 1384 1265 624 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 2355 2752 4357 5334 1203 Size: 1152 397 1605 977 1327 Fragments arranged by size: 1605 1327 1152 977 397 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5458 AlwNI CAG_nnn'CTG Cuts at: 3201 4773 3201 Size: 1572 3886 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2206 2507 2767 2993 3145 5254 1493 Size: 713 301 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 713 301 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2783 1870 Size: 73 840 4545 Fragments arranged by size: 4545 840 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2170 2779 4234 618 Size: 325 217 627 260 123 609 1455 1842 Fragments arranged by size: 1842 1455 627 609 325 260 217 123 BanII G_rGCy'C Cuts at: 1391 2699 2777 1391 Size: 1308 78 4072 Fragments arranged by size: 4072 1308 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4069 136 Size: 107 122 71 631 605 2397 1525 Fragments arranged by size: 2397 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 2052 2071 2173 3519 4675 1790 Size: 262 19 102 1346 1156 2573 Fragments arranged by size: 2573 1346 1156 262 102 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 2737 3189 Size: 416 53 83 399 24 685 635 452 Cuts at: 3189 4147 442 Size: 958 1753 Fragments arranged by size: 1753 958 685 635 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4118 1239 Size: 2879 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4121 882 Size: 617 2622 2219 Fragments arranged by size: 2622 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2455 3249 493 Size: 711 1251 794 2702 Fragments arranged by size: 2702 1251 794 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3265 3647 4282 275 Size: 53 83 186 936 1732 382 635 1451 Fragments arranged by size: 1732 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5233 Fragments arranged by size: 5233 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 1186 Size: 877 4581 BseYI C'CCAG_C Cuts at: 2674 4661 5157 2674 Size: 1987 496 2975 Fragments arranged by size: 2975 1987 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4885 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3519 3604 4675 3519 Size: 85 1071 4302 Fragments arranged by size: 4302 1071 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2071 2173 2699 2777 3519 3604 Size: 399 262 19 102 526 78 742 85 Cuts at: 3604 4675 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 262 102 85 78 19 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 2813 844 Size: 1457 512 3489 Fragments arranged by size: 3489 1457 512 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2337 3345 1394 Size: 429 215 299 1008 3507 Fragments arranged by size: 3507 1008 429 299 215 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4102 4289 1700 Size: 110 2292 187 2869 Fragments arranged by size: 2869 2292 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5458 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstXI CCAn_nnnn'nTGG Cuts at: 2674 2755 2674 Size: 81 5377 BstYI r'GATC_y Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453 Size: 816 367 385 17 1452 86 12 346 Cuts at: 5453 1972 Size: 1977 Fragments arranged by size: 1977 1452 816 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2578 513 Size: 552 1513 3393 Fragments arranged by size: 3393 1513 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2402 487 Size: 683 56 1176 3543 Fragments arranged by size: 3543 1176 683 56 BtsI GCAGTG_nn' Cuts at: 2099 2661 2974 3768 3788 2099 Size: 562 313 794 20 3769 Fragments arranged by size: 3769 794 562 313 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DraIII CAC_nnn'GTG Cuts at: 2455 2455 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2473 2905 3794 Size: 54 1425 50 275 18 643 432 889 Cuts at: 3794 5423 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 643 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2188 3388 1353 Size: 835 1200 3423 Fragments arranged by size: 3423 1200 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4045 4419 4565 387 Size: 1064 138 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 3521 4118 4905 5173 1239 Size: 393 624 1265 597 787 268 1524 Fragments arranged by size: 1524 1265 787 624 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2534 2534 Size: 5458 EcoO109I rG'GnC_Cy Cuts at: 2225 2225 Size: 5458 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2546 2572 2546 Size: 26 5432 FspI TGC'GCA Cuts at: 3964 3964 Size: 5458 HaeII r_GCGC'y Cuts at: 1678 4605 1678 Size: 2927 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373 Size: 32 135 32 2527 32 42 32 2626 Fragments arranged by size: 2626 2527 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 3048 669 Size: 988 281 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 2150 748 Size: 1402 4056 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4572 4756 1305 Size: 3267 184 2007 Fragments arranged by size: 3267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3416 3775 3934 518 Size: 648 2250 359 159 2042 Fragments arranged by size: 2250 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2580 2874 3551 4699 4944 1606 Size: 974 294 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 677 294 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4906 NdeI CA'TA_TG Cuts at: 387 2366 2701 387 Size: 1979 335 3144 Fragments arranged by size: 3144 1979 335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2359 2674 2746 2816 4361 1132 Size: 97 1130 315 72 70 1545 2229 Fragments arranged by size: 2229 1545 1130 315 97 72 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5458 PciI A'CATG_T Cuts at: 2355 4357 2355 Size: 2002 3456 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5040 5072 3527 Size: 32 1481 32 3913 Fragments arranged by size: 3913 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2225 2225 Size: 5458 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5458 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PstI C_TGCA'G Cuts at: 830 2810 830 Size: 1980 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 SacII CC_GC'GG Cuts at: 2581 2581 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 2691 3706 5418 2691 Size: 1015 1712 2731 Fragments arranged by size: 2731 1712 1015 SexAI A'CCwGG_T Cuts at: 2535 2535 Size: 5458 SfcI C'TryA_G Cuts at: 826 1045 2806 3941 4622 4813 826 Size: 219 1761 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2785 1872 Size: 913 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3562 4463 4725 820 Size: 197 926 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5458 StyI C'CwwG_G Cuts at: 513 1065 2897 513 Size: 552 1832 3074 Fragments arranged by size: 3074 1832 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2483 3485 3502 3655 3840 4285 Size: 1255 690 213 1002 17 153 185 445 Cuts at: 4285 325 Size: 1498 Fragments arranged by size: 1498 1255 1002 690 445 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2689 3704 5416 Size: 274 80 33 51 1443 712 1015 1712 Cuts at: 5416 96 Size: 138 Fragments arranged by size: 1712 1443 1015 712 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5418 5450 5418 Size: 32 5426 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2587 2794 2587 Size: 207 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2783 1870 Size: 913 4545 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI