(Linear) (Six-base) MAPSORT of: KIEE5236.seq Check: 4777 from: 1 to: 753 KIEE5236.orf With 182 enzymes: SgfI * January 29, 2014 11:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 753 Size: 251 502 AflIII A'CryG_T Cuts at: 0 350 747 753 Size: 350 397 6 Fragments arranged by size: 397 350 6 ApoI r'AATT_y Cuts at: 0 201 502 753 Size: 201 301 251 Fragments arranged by size: 301 251 201 BanI G'GyrC_C Cuts at: 0 42 165 753 Size: 42 123 588 Fragments arranged by size: 588 123 42 BanII G_rGCy'C Cuts at: 0 694 753 Size: 694 59 Bme1580I G_kGCm'C Cuts at: 0 47 66 168 753 Size: 47 19 102 585 Fragments arranged by size: 585 102 47 19 BmrI ACTGGGnnnn_n' Cuts at: 0 97 732 753 Size: 97 635 21 Fragments arranged by size: 635 97 21 BsaAI yAC'GTr Cuts at: 0 450 753 Size: 450 303 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 753 Size: 58 695 BseYI C'CCAG_C Cuts at: 0 669 753 Size: 669 84 Bsp1286I G_dGCh'C Cuts at: 0 47 66 168 694 753 Size: 47 19 102 526 59 Fragments arranged by size: 526 102 59 47 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 296 753 Size: 296 457 BsrBI CCG'CTC Cuts at: 0 33 332 753 Size: 33 299 421 Fragments arranged by size: 421 299 33 BstXI CCAn_nnnn'nTGG Cuts at: 0 669 753 Size: 669 84 BtgI C'CryG_G Cuts at: 0 573 753 Size: 573 180 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 397 753 Size: 397 356 BtsI GCAGTG_nn' Cuts at: 0 94 656 753 Size: 94 562 97 Fragments arranged by size: 562 97 94 DraIII CAC_nnn'GTG Cuts at: 0 450 753 Size: 450 303 EaeI y'GGCC_r Cuts at: 0 468 753 Size: 468 285 EarI CTCTTCn'nnn_ Cuts at: 0 183 753 Size: 183 570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 753 Size: 251 502 EcoNI CCTnn'n_nnAGG Cuts at: 0 529 753 Size: 529 224 EcoO109I rG'GnC_Cy Cuts at: 0 220 753 Size: 220 533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 541 567 753 Size: 541 26 186 Fragments arranged by size: 541 186 26 HindIII A'AGCT_T Cuts at: 0 145 753 Size: 145 608 MspA1I CmG'CkG Cuts at: 0 575 753 Size: 575 178 NdeI CA'TA_TG Cuts at: 0 361 696 753 Size: 361 335 57 Fragments arranged by size: 361 335 57 NspI r_CATG'y Cuts at: 0 354 669 741 753 Size: 354 315 72 12 Fragments arranged by size: 354 315 72 12 PciI A'CATG_T Cuts at: 0 350 753 Size: 350 403 PpuMI rG'GwC_Cy Cuts at: 0 220 753 Size: 220 533 SacII CC_GC'GG Cuts at: 0 576 753 Size: 576 177 ScaI AGT'ACT Cuts at: 0 686 753 Size: 686 67 SexAI A'CCwGG_T Cuts at: 0 530 753 Size: 530 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 478 753 Size: 265 213 275 Fragments arranged by size: 275 265 213 TatI w'GTAC_w Cuts at: 0 684 753 Size: 684 69 XbaI T'CTAG_A Cuts at: 0 582 753 Size: 582 171 Enzymes that do cut: AcuI AflIII ApoI BanI BanII Bme1580I BmrI BsaAI BseRI BseYI Bsp1286I BspMI BsrBI BstXI BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HindIII MspA1I NdeI NspI PciI PpuMI SacII ScaI SexAI TaqII TatI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XcmI XhoI XmaI XmnI ZraI