################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwNI 78 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 14 GGGCCC GGGCC^C AvaII 153 GGACC G^G[AT]CC BamHI 3 GGATCC G^GATCC BpmI 37 CTGGAG CTGGAG(16/14) BsaJI 131 CCAAGG C^C[ACGT][ACGT]GG BsaWI 89 ACCGGA [AT]^CCGG[AT] BseRI 104 CTCCTC GAGGAG(10/8) BsgI 247 GTGCAG GTGCAG(16/14) BsmAI 163 GTCTC GTCTC(1/5) Bsp1407I 62 TGTACA T^GTACA BsrBI 187 GAGCGG CCGCTC(-3/-3) BsrGI 62 TGTACA T^GTACA BsrI 77 CCAGT ACTGG(1/-1) BstYI 3 GGATCC [GA]^GATC[CT] DpnII 4 GATC ^GATC DraII 13 AGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 53 TGGCCG [CT]^GGCC[GA] EcoO109I 13 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 131 CCAAGG C^C[AT][AT]GG HapII 90 CCGG C^CGG HincII 204 GTTGAC GT[CT]^[GA]AC HindII 204 GTTGAC GT[CT]^[GA]AC HinfI 139 GAGTC G^A[ACGT]TC HpaII 90 CCGG C^CGG MboI 4 GATC ^GATC MflI 3 GGATCC [GA]^GATC[CT] MspA1I 22 CAGCTG C[AC]G^C[GT]G MspI 90 CCGG C^CGG NdeII 4 GATC ^GATC NlaIII 184 CATG CATG^ PflMI 18 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 139 GAGTC GAGTC(4/5) PvuII 22 CAGCTG CAG^CTG SapI 229 GAAGAGC GCTCTTC(1/4) Sau3AI 4 GATC ^GATC SspBI 62 TGTACA T^GTACA StyI 131 CCAAGG C^C[AT][AT]GG TspRI 78 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 18 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 3 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 31 GCGG CCGC(-3/-1) 189 GCGG AfaI 63 GTAC GT^AC 171 GTAC AlwI 4 GATCC GGATC(4/5) 3 GGATC AspHI 124 GAGCTC G[AT]GC[AT]^C 255 GAGCTC BbvI 249 GCAGC GCAGC(8/12) 28 GCTGC BsiHKAI 124 GAGCTC G[AT]GC[AT]^C 255 GAGCTC BsiYI 18 CCAACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 83 CCTGCCACCGG BslI 18 CCAACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 83 CCTGCCACCGG BssKI 150 CCTGG ^CC[ACGT]GG 174 CCTGG BstNI 150 CCTGG CC^[AT]GG 174 CCTGG DdeI 10 CTCAG C^T[ACGT]AG 167 CTAAG DrdI 154 GACCGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 207 GACAGAGTTGTC EarI 229 GAAGAG CTCTTC(1/4) 240 GAAGAG EcoRII 150 CCTGG ^CC[AT]GG 174 CCTGG Fnu4HI 28 GCTGC GC^[ACGT]GC 249 GCAGC ItaI 28 GCTGC GC^[ACGT]GC 249 GCAGC Ksp632I 229 GAAGAG CTCTTC(1/4) 240 GAAGAG MboII 229 GAAGA GAAGA(8/7) 240 GAAGA MvaI 150 CCTGG CC^[AT]GG 174 CCTGG NlaIV 3 GGATCC GG[ACGT]^[ACGT]CC 14 GGGCCC RsaI 63 GTAC GT^AC 171 GTAC SacI 124 GAGCTC GAGCT^C 255 GAGCTC ScrFI 150 CCTGG CC^[ACGT]GG 174 CCTGG TseI 28 GCTGC G^C[AT]GC 249 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BanII BmyI Bsp1286I BstF5I Cac8I Cfr13I FokI HaeIII MnlI Sau96I