(Circular) (Six-base) MAPSORT of: pFN21AE6193.seq Check: 5224 from: 1 to: 4975 pFN21AE6193 4975 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2785 278 Size: 53 83 186 936 1249 2468 Fragments arranged by size: 2468 1249 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2318 1937 Size: 381 4594 Acc65I G'GTAC_C Cuts at: 2296 2296 Size: 4975 AclI AA'CG_TT Cuts at: 1563 3102 3475 1563 Size: 1539 373 3063 Fragments arranged by size: 3063 1539 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3038 4422 4690 1632 Size: 1406 1384 268 1917 Fragments arranged by size: 1917 1406 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4778 AflIII A'CryG_T Cuts at: 1203 3874 4851 1203 Size: 2671 977 1327 Fragments arranged by size: 2671 1327 977 AgeI A'CCGG_T Cuts at: 3806 3806 Size: 4975 AhdI GACnn_n'nnGTC Cuts at: 3704 3704 Size: 4975 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4975 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4975 AlwNI CAG_nnn'CTG Cuts at: 2088 2718 4290 2088 Size: 630 1572 2773 Fragments arranged by size: 2773 1572 630 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 4975 ApaLI G'TGCA_C Cuts at: 3032 4188 3032 Size: 1156 3819 ApoI r'AATT_y Cuts at: 1493 2284 2510 2662 4771 1493 Size: 791 226 152 2109 1697 Fragments arranged by size: 2109 1697 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 3529 160 Size: 872 2497 1606 Fragments arranged by size: 2497 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4975 AvaI C'yCGr_G Cuts at: 1870 1943 2300 1870 Size: 73 357 4545 Fragments arranged by size: 4545 357 73 BamHI G'GATC_C Cuts at: 2008 2305 2672 2008 Size: 297 367 4311 Fragments arranged by size: 4311 367 297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2296 3751 618 Size: 325 217 627 509 1455 1842 Fragments arranged by size: 1842 1455 627 509 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2133 2264 2294 1391 Size: 632 110 131 30 4072 Fragments arranged by size: 4072 632 131 110 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3166 3200 1903 Size: 34 1229 34 3678 Fragments arranged by size: 3678 1229 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2867 4077 2867 Size: 1210 3765 BclI T'GATC_A Cuts at: 1548 1548 Size: 4975 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3586 136 Size: 107 122 71 631 605 1914 1525 Fragments arranged by size: 1914 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4970 4970 Size: 4975 BlpI GC'TnA_GC Cuts at: 2392 2392 Size: 4975 Bme1580I G_kGCm'C Cuts at: 1790 2023 3036 4192 1790 Size: 233 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4975 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2706 3664 442 Size: 416 53 83 399 24 1289 958 1753 Fragments arranged by size: 1753 1289 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4975 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 3635 1239 Size: 824 1572 2579 Fragments arranged by size: 2579 1572 824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3100 3965 4263 3100 Size: 865 298 3812 Fragments arranged by size: 3812 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3638 882 Size: 617 2139 2219 Fragments arranged by size: 2219 2139 617 BsaAI yAC'GTr Cuts at: 493 1204 2766 493 Size: 711 1562 2702 Fragments arranged by size: 2702 1562 711 BsaBI GATnn'nnATC Cuts at: 4969 4969 Size: 4975 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2782 3164 3799 275 Size: 53 83 186 936 1249 382 635 1451 Fragments arranged by size: 1451 1249 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 3408 3806 4080 4227 4638 2094 Size: 1314 398 274 147 411 2431 Fragments arranged by size: 2431 1314 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4750 Fragments arranged by size: 4750 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 1186 Size: 914 4061 BseYI C'CCAG_C Cuts at: 4178 4674 4178 Size: 496 4479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 1163 Size: 573 537 3865 Fragments arranged by size: 3865 573 537 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2425 3186 4214 1540 Size: 275 186 424 761 1028 2301 Fragments arranged by size: 2301 1028 761 424 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2264 3036 3121 4192 2133 Size: 131 772 85 1071 2916 Fragments arranged by size: 2916 1071 772 131 85 BsmI GAATG_Cn' Cuts at: 2486 2579 2486 Size: 93 4882 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2133 2264 2294 3036 3121 4192 Size: 399 233 110 131 30 742 85 1071 Cuts at: 4192 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 233 131 110 85 30 BspEI T'CCGG_A Cuts at: 4638 4638 Size: 4975 BspHI T'CATG_A Cuts at: 2864 4594 2864 Size: 1730 3245 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 844 Size: 1486 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2194 2862 1394 Size: 429 371 668 3507 Fragments arranged by size: 3507 668 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 3470 3644 65 Size: 1183 2222 174 1396 Fragments arranged by size: 2222 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3619 3806 1700 Size: 110 1809 187 2869 Fragments arranged by size: 2869 1809 187 110 BsrGI T'GTAC_A Cuts at: 96 2067 96 Size: 1971 3004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4975 BssSI C'ACGA_G Cuts at: 3035 4047 3035 Size: 1012 3963 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4975 BstBI TT'CG_AA Cuts at: 3779 3779 Size: 4975 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4975 BstYI r'GATC_y Cuts at: 1972 2008 2305 2672 3057 3074 4526 4612 4624 Size: 36 297 367 385 17 1452 86 12 Cuts at: 4624 4970 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 297 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3484 Fragments arranged by size: 3484 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4236 Fragments arranged by size: 4236 683 56 BtsI GCAGTG_nn' Cuts at: 2491 3285 3305 2491 Size: 794 20 4161 Fragments arranged by size: 4161 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4975 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4940 DraI TTT'AAA Cuts at: 2279 2279 Size: 4975 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2165 2218 3982 809 Size: 507 849 53 1764 1802 Fragments arranged by size: 1802 1764 849 507 53 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2422 3311 Size: 54 1425 50 275 18 228 364 889 Cuts at: 3311 4940 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 364 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2422 1537 Size: 275 610 4090 Fragments arranged by size: 4090 610 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2240 2905 1353 Size: 876 11 665 3423 Fragments arranged by size: 3423 876 665 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3562 3936 4082 387 Size: 1064 138 1973 374 146 1280 Fragments arranged by size: 1973 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2262 2131 Size: 131 4844 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 3038 3635 4422 4690 1239 Size: 393 431 975 597 787 268 1524 Fragments arranged by size: 1524 975 787 597 431 393 268 EcoO109I rG'GnC_Cy Cuts at: 2019 2019 Size: 4975 EcoRI G'AATT_C Cuts at: 2284 2662 2284 Size: 378 4597 FspI TGC'GCA Cuts at: 3481 3481 Size: 4975 HaeII r_GCGC'y Cuts at: 1678 4122 1678 Size: 2444 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3616 3648 3690 3722 1373 Size: 32 135 32 2044 32 42 32 2626 Fragments arranged by size: 2626 2044 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2211 2319 2565 669 Size: 988 281 273 108 246 3079 Fragments arranged by size: 3079 988 281 273 246 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 4975 HpaI GTT'AAC Cuts at: 2565 2565 Size: 4975 KpnI G_GTAC'C Cuts at: 2300 2300 Size: 4975 MfeI C'AATT_G Cuts at: 2574 2574 Size: 4975 MluI A'CGCG_T Cuts at: 4851 4851 Size: 4975 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4089 4273 1305 Size: 2784 184 2007 Fragments arranged by size: 2784 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3153 Fragments arranged by size: 3153 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2933 3292 3451 518 Size: 648 1767 359 159 2042 Fragments arranged by size: 2042 1767 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2391 3068 4216 4461 1606 Size: 423 362 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 423 362 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3484 Fragments arranged by size: 3484 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4975 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4975 NotI GC'GGCC_GC Cuts at: 2422 2422 Size: 4975 NspI r_CATG'y Cuts at: 1132 1229 2333 3878 1132 Size: 97 1104 1545 2229 Fragments arranged by size: 2229 1545 1104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4975 PciI A'CATG_T Cuts at: 3874 3874 Size: 4975 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 1108 Size: 921 4054 PmeI GTTT'AAAC Cuts at: 2279 2279 Size: 4975 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3044 3076 4557 4589 3044 Size: 32 1481 32 3430 Fragments arranged by size: 3430 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4975 PsiI TTA'TAA Cuts at: 2545 2545 Size: 4975 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 4975 PstI C_TGCA'G Cuts at: 830 2275 2327 830 Size: 1445 52 3478 Fragments arranged by size: 3478 1445 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4975 PvuII CAG'CTG Cuts at: 2029 2029 Size: 4975 SacI G_AGCT'C Cuts at: 2133 2264 2133 Size: 131 4844 SalI G'TCGA_C Cuts at: 1936 2317 1936 Size: 381 4594 SapI GCTCTTCn'nnn_ Cuts at: 2229 2229 Size: 4975 SbfI CC_TGCA'GG Cuts at: 2327 2327 Size: 4975 ScaI AGT'ACT Cuts at: 3223 4935 3223 Size: 1712 3263 SfcI C'TryA_G Cuts at: 826 1045 2271 2323 3458 4139 4330 826 Size: 219 1226 52 1135 681 191 1471 Fragments arranged by size: 1471 1226 1135 681 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4975 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4975 SmaI CCC'GGG Cuts at: 1872 2302 1872 Size: 430 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3079 3980 4242 820 Size: 197 926 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2766 493 Size: 2273 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4975 SphI G_CATG'C Cuts at: 1132 2333 1132 Size: 1201 3774 SspI AAT'ATT Cuts at: 5 52 2899 5 Size: 47 2847 2081 Fragments arranged by size: 2847 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4975 StyI C'CwwG_G Cuts at: 513 1065 2004 2136 2414 513 Size: 552 939 132 278 3074 Fragments arranged by size: 3074 939 552 278 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 3002 3019 3172 3357 3802 325 Size: 1255 595 827 17 153 185 445 1498 Fragments arranged by size: 1498 1255 827 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 3221 4933 Size: 274 80 33 51 1443 90 1154 1712 Cuts at: 4933 96 Size: 138 Fragments arranged by size: 1712 1443 1154 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4935 4967 4935 Size: 32 4943 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4975 XbaI T'CTAG_A Cuts at: 2311 2311 Size: 4975 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4975 XmaI C'CCGG_G Cuts at: 1870 2300 1870 Size: 430 4545 XmnI GAAnn'nnTTC Cuts at: 3104 3104 Size: 4975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2783 276 Size: 53 83 186 936 1249 2468 Fragments arranged by size: 2468 1249 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI