################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbvCI BciVI BfaI BfrI BglII BlnI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 447 AACGTT AA^CGTT AcyI 1156 GACGCC G[GA]^CG[CT]C AflIII 473 ACATGT A^C[GA][CT]GT AlwNI 330 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 673 CCTGAGG CC^T[ACGT]AGG ApaI 1638 GGGCCC GGGCC^C AspHI 256 GTGCTC G[AT]GC[AT]^C AvaI 374 CCCGGG C^[CT]CG[GA]G BamHI 284 GGATCC G^GATCC BanI 847 GGCACC G^G[CT][GA]CC BbrPI 144 CACGTG CAC^GTG BbsI 1072 GTCTTC GAAGAC(2/6) BcgI 1172 GCACTGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 110 TGATCA T^GATCA BpuAI 1072 GTCTTC GAAGAC(2/6) BsaAI 144 CACGTG [CT]AC^GT[GA] BsaHI 1156 GACGCC G[GA]^CG[CT]C BsaWI 26 ACCGGA [AT]^CCGG[AT] BsiHKAI 256 GTGCTC G[AT]GC[AT]^C BsoBI 374 CCCGGG C^[CT]CG[GA]G BspHI 509 TCATGA T^CATGA BspLU11I 473 ACATGT A^CATGT BsrFI 571 GCCGGT [GA]^CCGG[CT] BstAPI 329 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 673 CCTGAGG CC^T[ACGT]AGG Cfr10I 571 GCCGGT [GA]^CCGG[CT] DraIII 727 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 673 CCTGAGG CC^T[ACGT]AGG FbaI 110 TGATCA T^GATCA Hin1I 1156 GACGCC G[GA]^CG[CT]C MseI 499 TTAA T^TAA NspI 473 ACATGT [GA]CATG^[CT] PleI 1392 GAGTC GAGTC(4/5) PmaCI 144 CACGTG CAC^GTG PmlI 144 CACGTG CAC^GTG PpuMI 1506 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1341 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 447 AACGTT AA^CGTT RcaI 509 TCATGA T^CATGA SexAI 601 ACCTGGT A^CC[AT]GGT SmaI 374 CCCGGG CCC^GGG StuI 1440 AGGCCT AGG^CCT Tru9I 499 TTAA T^TAA Tsp509I 685 AATT ^AATT XmaCI 374 CCCGGG C^CCGGG XmaI 374 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BalI 196 TGGCCA TGG^CCA 1005 TGGCCA BglI 46 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 571 GCCGGTGTGGC BseRI 922 CTCCTC GAGGAG(10/8) 1065 CTCCTC BstEII 739 GGTGACC G^GT[ACGT]ACC 1668 GGTGACC BstPI 739 GGTGACC G^GT[ACGT]ACC 1668 GGTGACC BstXI 494 CCACGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1628 CCAATGGGATGG BstYI 284 GGATCC [GA]^GATC[CT] 1235 AGATCC DraII 791 AGGCCCC [GA]G^G[ACGT]CC[CT] 1506 GGGACCC DsaI 767 CCATGG C^C[GA][CT]GG 929 CCATGG EaeI 196 TGGCCA [CT]^GGCC[GA] 1005 TGGCCA EcoO109I 791 AGGCCCC [GA]G^G[ACGT]CC[CT] 1506 GGGACCC EcoO65I 739 GGTGACC G^GT[ACGT]ACC 1668 GGTGACC EcoT14I 767 CCATGG C^C[AT][AT]GG 929 CCATGG MflI 284 GGATCC [GA]^GATC[CT] 1235 AGATCC MluNI 196 TGGCCA TGG^CCA 1005 TGGCCA MscI 196 TGGCCA TGG^CCA 1005 TGGCCA NcoI 767 CCATGG C^CATGG 929 CCATGG PstI 1403 CTGCAG CTGCA^G 1444 CTGCAG SfcI 1403 CTGCAG C^T[GA][CT]AG 1444 CTGCAG StyI 767 CCATGG C^C[AT][AT]GG 929 CCATGG TaqI 656 TCGA T^CGA 1365 TCGA TthHB8I 656 TCGA T^CGA 1365 TCGA XcmI 269 CCACCCTGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1377 CCACTCAGCCTGTGG XhoII 284 GGATCC [GA]^GATC[CT] 1235 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I AvaII BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI XmnI