(Circular) (Six-base) MAPSORT of: pFN21AE6488.seq Check: 7445 from: 1 to: 6382 pFN21AE6488 6382 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2187 3119 2187 Size: 932 5450 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 2453 4509 4882 1563 Size: 890 2056 373 3063 Fragments arranged by size: 3063 2056 890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3467 3671 4445 5829 6097 1632 Size: 512 1323 204 774 1384 268 1917 Fragments arranged by size: 1917 1384 1323 774 512 268 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 2478 5281 6258 1203 Size: 1275 2803 977 1327 Fragments arranged by size: 2803 1327 1275 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 2060 2762 1166 Size: 894 702 4786 Fragments arranged by size: 4786 894 702 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2078 1184 Size: 894 5488 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2480 Size: 32 6350 AlwNI CAG_nnn'CTG Cuts at: 2340 4125 5697 2340 Size: 1785 1572 3025 Fragments arranged by size: 3025 1785 1572 ApaI G_GGCC'C Cuts at: 3647 3647 Size: 6382 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 3691 3917 4069 6178 1493 Size: 2198 226 152 2109 1697 Fragments arranged by size: 2198 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 2379 3707 1870 Size: 73 436 1328 4545 Fragments arranged by size: 4545 1328 436 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2012 2045 2012 Size: 33 6349 BamHI G'GATC_C Cuts at: 2289 3712 4079 2289 Size: 1423 367 4592 Fragments arranged by size: 4592 1423 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2852 3703 5158 618 Size: 325 217 627 1065 851 1455 1842 Fragments arranged by size: 1842 1455 1065 851 627 325 217 BanII G_rGCy'C Cuts at: 1391 2108 3531 3647 3701 1391 Size: 717 1423 116 54 4072 Fragments arranged by size: 4072 1423 717 116 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3070 928 Size: 971 1171 4240 Fragments arranged by size: 4240 1171 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3166 3200 4573 4607 1903 Size: 34 1229 34 1373 34 3678 Fragments arranged by size: 3678 1373 1229 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 2115 1548 Size: 567 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 2582 4993 Size: 107 122 71 631 605 385 525 2411 Cuts at: 4993 136 Size: 1525 Fragments arranged by size: 2411 1525 631 605 525 385 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 2264 2786 3659 3799 2264 Size: 522 873 140 4847 Fragments arranged by size: 4847 873 522 140 Bme1580I G_kGCm'C Cuts at: 1790 2156 2855 3179 3610 3647 4443 5599 1790 Size: 366 699 324 431 37 796 1156 2573 Fragments arranged by size: 2573 1156 796 699 431 366 324 37 BmgBI CAC'GTC Cuts at: 1318 2386 1318 Size: 1068 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 4113 5071 Size: 416 53 83 399 24 877 1819 958 Cuts at: 5071 442 Size: 1753 Fragments arranged by size: 1819 1753 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2828 3011 3320 3599 5042 1239 Size: 848 741 183 309 279 1443 2579 Fragments arranged by size: 2579 1443 848 741 309 279 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4507 5372 5670 4507 Size: 865 298 5219 Fragments arranged by size: 5219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5045 882 Size: 617 3546 2219 Fragments arranged by size: 3546 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2151 4173 493 Size: 711 947 2022 2702 Fragments arranged by size: 2702 2022 947 711 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3162 4189 4571 5206 Size: 53 83 186 936 1629 1027 382 635 Cuts at: 5206 275 Size: 1451 Fragments arranged by size: 1629 1451 1027 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 4815 5213 5487 5634 6045 2031 Size: 2784 398 274 147 411 2368 Fragments arranged by size: 2784 2368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2480 2510 1178 Size: 30 165 30 1077 30 5050 Fragments arranged by size: 5050 1077 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 3061 1186 Size: 1732 143 4507 Fragments arranged by size: 4507 1732 143 BseYI C'CCAG_C Cuts at: 2100 2155 2801 2946 5585 6081 2100 Size: 55 646 145 2639 496 2401 Fragments arranged by size: 2639 2401 646 496 145 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2038 2341 2626 3341 1163 Size: 573 302 303 285 715 4204 Fragments arranged by size: 4204 715 573 303 302 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 4443 4528 5599 2265 Size: 2178 85 1071 3048 Fragments arranged by size: 3048 2178 1071 85 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2156 2265 2855 3179 3531 3610 Size: 399 318 48 109 590 324 352 79 Cuts at: 3610 3647 3701 4443 4528 5599 1391 Size: 37 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 590 399 352 324 318 109 85 79 54 48 37 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 2514 4271 6001 2514 Size: 1757 1730 2895 Fragments arranged by size: 2895 1757 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 2499 3119 3294 3737 844 Size: 1343 312 620 175 443 3489 Fragments arranged by size: 3489 1343 620 443 312 175 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4877 5051 65 Size: 1183 3629 174 1396 Fragments arranged by size: 3629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2576 5026 5213 1700 Size: 110 766 2450 187 2869 Fragments arranged by size: 2869 2450 766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 2340 794 Size: 1546 4836 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 2744 3673 1184 Size: 1560 929 3893 Fragments arranged by size: 3893 1560 929 BstXI CCAn_nnnn'nTGG Cuts at: 2506 3640 2506 Size: 1134 5248 BstYI r'GATC_y Cuts at: 1972 2289 3240 3712 4079 4464 4481 5933 6019 Size: 317 951 472 367 385 17 1452 86 Cuts at: 6019 6031 6377 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 951 472 385 367 346 317 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2679 2679 Size: 6382 BtgI C'CryG_G Cuts at: 513 1065 2004 2772 2934 513 Size: 552 939 768 162 3961 Fragments arranged by size: 3961 939 768 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3518 487 Size: 683 56 2292 3351 Fragments arranged by size: 3351 2292 683 56 BtsI GCAGTG_nn' Cuts at: 2341 2346 3417 3898 4692 4712 2341 Size: 5 1071 481 794 20 4011 Fragments arranged by size: 4011 1071 794 481 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2695 2730 529 Size: 35 2131 35 4181 Fragments arranged by size: 4181 2131 35 35 DraI TTT'AAA Cuts at: 3686 3686 Size: 6382 DraIII CAC_nnn'GTG Cuts at: 2737 2737 Size: 6382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5389 809 Size: 507 4073 1802 Fragments arranged by size: 4073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2201 3010 3829 Size: 54 1425 50 275 18 371 809 819 Cuts at: 3829 4718 6347 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 819 809 371 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2539 2756 3599 4312 1353 Size: 728 458 217 843 713 3423 Fragments arranged by size: 3423 843 728 713 458 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3157 4969 5343 5489 387 Size: 1064 138 1568 1812 374 146 1280 Fragments arranged by size: 1812 1568 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2144 2828 3011 3320 3467 3599 Size: 393 455 57 684 183 309 147 132 Cuts at: 3599 3671 4445 5042 5829 6097 1239 Size: 72 774 597 787 268 1524 Fragments arranged by size: 1524 787 774 684 597 455 393 309 268 183 147 132 72 57 EcoO109I rG'GnC_Cy Cuts at: 2797 3512 2797 Size: 715 5667 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 2041 2271 2420 5529 1678 Size: 363 230 149 3109 2531 Fragments arranged by size: 3109 2531 363 230 149 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5023 5055 5097 5129 1373 Size: 32 135 32 3451 32 42 32 2626 Fragments arranged by size: 3451 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3726 3972 669 Size: 988 281 1788 246 3079 Fragments arranged by size: 3079 1788 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6382 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3447 5496 5680 1305 Size: 2142 2049 184 2007 Fragments arranged by size: 2142 2049 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2203 3012 10 Size: 54 1425 343 371 809 3380 Fragments arranged by size: 3380 1425 809 371 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2363 2504 2762 2939 3503 4340 Size: 648 894 303 141 258 177 564 837 Cuts at: 4340 4699 4858 518 Size: 359 159 2042 Fragments arranged by size: 2042 894 837 648 564 359 303 258 177 159 141 MspA1I CmG'CkG Cuts at: 1606 2159 2950 3094 3798 4475 5623 5868 1606 Size: 553 791 144 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 704 677 553 245 144 NcoI C'CATG_G Cuts at: 513 1065 2004 2772 2934 513 Size: 552 939 768 162 3961 Fragments arranged by size: 3961 939 768 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 6382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 2482 3740 5285 1132 Size: 97 1253 1258 1545 2229 Fragments arranged by size: 2229 1545 1258 1253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 2478 5281 2478 Size: 2803 3579 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PmlI CAC'GTG Cuts at: 2151 2151 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2512 4451 4483 5964 5996 2480 Size: 32 1939 32 1481 32 2866 Fragments arranged by size: 2866 1939 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3512 3512 Size: 6382 PshAI GACnn'nnGTC Cuts at: 1613 3350 1613 Size: 1737 4645 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PspOMI G'GGCC_C Cuts at: 3643 3643 Size: 6382 PstI C_TGCA'G Cuts at: 830 3412 3453 3734 830 Size: 2582 41 281 3478 Fragments arranged by size: 3478 2582 281 41 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 PvuII CAG'CTG Cuts at: 2159 2950 3094 2159 Size: 791 144 5447 Fragments arranged by size: 5447 791 144 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SanDI GG'GwC_CC Cuts at: 3512 3512 Size: 6382 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SexAI A'CCwGG_T Cuts at: 2606 2606 Size: 6382 SfcI C'TryA_G Cuts at: 826 1045 3408 3449 3730 4865 5546 5737 826 Size: 219 2363 41 281 1135 681 191 1471 Fragments arranged by size: 2363 1471 1135 681 281 219 191 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 2381 3709 1872 Size: 509 1328 4545 Fragments arranged by size: 4545 1328 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 4486 5387 5649 820 Size: 197 926 2543 901 262 1553 Fragments arranged by size: 2543 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 4306 5 Size: 47 4254 2081 Fragments arranged by size: 4254 2081 47 StuI AGG'CCT Cuts at: 1517 3447 1517 Size: 1930 4452 StyI C'CwwG_G Cuts at: 513 1065 2004 2772 2934 3821 513 Size: 552 939 768 162 887 3074 Fragments arranged by size: 3074 939 887 768 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 2774 3526 4409 4426 4579 4764 Size: 1255 615 579 752 883 17 153 185 Cuts at: 4764 5209 325 Size: 445 1498 Fragments arranged by size: 1498 1255 883 752 615 579 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2089 4628 6340 Size: 274 80 33 51 1443 112 2539 1712 Cuts at: 6340 96 Size: 138 Fragments arranged by size: 2539 1712 1443 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 6342 6374 2274 Size: 32 4036 32 2282 Fragments arranged by size: 4036 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 3389 2281 Size: 1108 5274 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 2379 3707 1870 Size: 509 1328 4545 Fragments arranged by size: 4545 1328 509 XmnI GAAnn'nnTTC Cuts at: 2075 2667 2865 4511 2075 Size: 592 198 1646 3946 Fragments arranged by size: 3946 1646 592 198 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI