(Linear) (Six-base) MAPSORT of: KIEE6488.seq Check: 8638 from: 1 to: 1677 KIEE6488.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 182 1114 1677 Size: 182 932 563 Fragments arranged by size: 932 563 182 AclI AA'CG_TT Cuts at: 0 448 1677 Size: 448 1229 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1462 1666 1677 Size: 139 1323 204 11 Fragments arranged by size: 1323 204 139 11 AflIII A'CryG_T Cuts at: 0 473 1677 Size: 473 1204 AleI CACnn'nnGTG Cuts at: 0 55 757 1677 Size: 55 702 920 Fragments arranged by size: 920 702 55 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 73 1677 Size: 73 1604 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 475 507 1677 Size: 475 32 1170 Fragments arranged by size: 1170 475 32 AlwNI CAG_nnn'CTG Cuts at: 0 335 1677 Size: 335 1342 ApaI G_GGCC'C Cuts at: 0 1642 1677 Size: 1642 35 AvaI C'yCGr_G Cuts at: 0 374 1677 Size: 374 1303 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 7 40 1677 Size: 7 33 1637 Fragments arranged by size: 1637 33 7 BamHI G'GATC_C Cuts at: 0 284 1677 Size: 284 1393 BanI G'GyrC_C Cuts at: 0 847 1677 Size: 847 830 BanII G_rGCy'C Cuts at: 0 103 1526 1642 1677 Size: 103 1423 116 35 Fragments arranged by size: 1423 116 103 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 1065 1677 Size: 1065 612 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1161 1195 1677 Size: 1161 34 482 Fragments arranged by size: 1161 482 34 BclI T'GATC_A Cuts at: 0 110 1677 Size: 110 1567 BglI GCCn_nnn'nGGC Cuts at: 0 52 577 1677 Size: 52 525 1100 Fragments arranged by size: 1100 525 52 BlpI GC'TnA_GC Cuts at: 0 259 781 1654 1677 Size: 259 522 873 23 Fragments arranged by size: 873 522 259 23 Bme1580I G_kGCm'C Cuts at: 0 151 850 1174 1605 1642 1677 Size: 151 699 324 431 37 35 Fragments arranged by size: 699 431 324 151 37 35 BmgBI CAC'GTC Cuts at: 0 381 1677 Size: 381 1296 BmrI ACTGGGnnnn_n' Cuts at: 0 289 1677 Size: 289 1388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 823 1006 1315 1594 1677 Size: 82 741 183 309 279 83 Fragments arranged by size: 741 309 279 183 83 82 BsaAI yAC'GTr Cuts at: 0 146 1677 Size: 146 1531 BsaHI Gr'CG_yC Cuts at: 0 1157 1677 Size: 1157 520 BsaWI w'CCGG_w Cuts at: 0 26 1677 Size: 26 1651 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 475 505 1677 Size: 475 30 1172 Fragments arranged by size: 1172 475 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 913 1056 1677 Size: 913 143 621 Fragments arranged by size: 913 621 143 BseYI C'CCAG_C Cuts at: 0 95 150 796 941 1677 Size: 95 55 646 145 736 Fragments arranged by size: 736 646 145 95 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 336 621 1336 1677 Size: 33 303 285 715 341 Fragments arranged by size: 715 341 303 285 33 BsiHKAI G_wGCw'C Cuts at: 0 260 1677 Size: 260 1417 Bsp1286I G_dGCh'C Cuts at: 0 103 151 260 850 1174 1526 1605 1642 Size: 103 48 109 590 324 352 79 37 Cuts at: 1642 1677 Size: 35 Fragments arranged by size: 590 352 324 109 103 79 48 37 35 BspHI T'CATG_A Cuts at: 0 509 1677 Size: 509 1168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 182 494 1114 1289 1677 Size: 182 312 620 175 388 Fragments arranged by size: 620 388 312 182 175 BsrFI r'CCGG_y Cuts at: 0 571 1677 Size: 571 1106 BstAPI GCAn_nnn'nTGC Cuts at: 0 335 1677 Size: 335 1342 BstEII G'GTnAC_C Cuts at: 0 739 1668 1677 Size: 739 929 9 Fragments arranged by size: 929 739 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 501 1635 1677 Size: 501 1134 42 Fragments arranged by size: 1134 501 42 BstYI r'GATC_y Cuts at: 0 284 1235 1677 Size: 284 951 442 Fragments arranged by size: 951 442 284 Bsu36I CC'TnA_GG Cuts at: 0 674 1677 Size: 674 1003 BtgI C'CryG_G Cuts at: 0 767 929 1677 Size: 767 162 748 Fragments arranged by size: 767 748 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1513 1677 Size: 1513 164 BtsI GCAGTG_nn' Cuts at: 0 336 341 1412 1677 Size: 336 5 1071 265 Fragments arranged by size: 1071 336 265 5 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 690 725 1677 Size: 690 35 952 Fragments arranged by size: 952 690 35 DraIII CAC_nnn'GTG Cuts at: 0 732 1677 Size: 732 945 EaeI y'GGCC_r Cuts at: 0 196 1005 1677 Size: 196 809 672 Fragments arranged by size: 809 672 196 EarI CTCTTCn'nnn_ Cuts at: 0 76 534 751 1594 1677 Size: 76 458 217 843 83 Fragments arranged by size: 843 458 217 83 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1152 1677 Size: 1152 525 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 139 823 1006 1315 1462 1594 1666 Size: 82 57 684 183 309 147 132 72 Cuts at: 1666 1677 Size: 11 Fragments arranged by size: 684 309 183 147 132 82 72 57 11 EcoO109I rG'GnC_Cy Cuts at: 0 792 1507 1677 Size: 792 715 170 Fragments arranged by size: 792 715 170 HaeII r_GCGC'y Cuts at: 0 36 266 415 1677 Size: 36 230 149 1262 Fragments arranged by size: 1262 230 149 36 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1442 1677 Size: 1442 235 MscI TGG'CCA Cuts at: 0 198 1007 1677 Size: 198 809 670 Fragments arranged by size: 809 670 198 MslI CAynn'nnrTG Cuts at: 0 55 358 499 757 934 1498 1677 Size: 55 303 141 258 177 564 179 Fragments arranged by size: 564 303 258 179 177 141 55 MspA1I CmG'CkG Cuts at: 0 154 945 1089 1677 Size: 154 791 144 588 Fragments arranged by size: 791 588 154 144 NcoI C'CATG_G Cuts at: 0 767 929 1677 Size: 767 162 748 Fragments arranged by size: 767 748 162 NspI r_CATG'y Cuts at: 0 477 1677 Size: 477 1200 PciI A'CATG_T Cuts at: 0 473 1677 Size: 473 1204 PmlI CAC'GTG Cuts at: 0 146 1677 Size: 146 1531 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 475 507 1677 Size: 475 32 1170 Fragments arranged by size: 1170 475 32 PpuMI rG'GwC_Cy Cuts at: 0 1507 1677 Size: 1507 170 PshAI GACnn'nnGTC Cuts at: 0 1345 1677 Size: 1345 332 PspOMI G'GGCC_C Cuts at: 0 1638 1677 Size: 1638 39 PstI C_TGCA'G Cuts at: 0 1407 1448 1677 Size: 1407 41 229 Fragments arranged by size: 1407 229 41 PvuII CAG'CTG Cuts at: 0 154 945 1089 1677 Size: 154 791 144 588 Fragments arranged by size: 791 588 154 144 SanDI GG'GwC_CC Cuts at: 0 1507 1677 Size: 1507 170 SexAI A'CCwGG_T Cuts at: 0 601 1677 Size: 601 1076 SfcI C'TryA_G Cuts at: 0 1403 1444 1677 Size: 1403 41 233 Fragments arranged by size: 1403 233 41 SmaI CCC'GGG Cuts at: 0 376 1677 Size: 376 1301 StuI AGG'CCT Cuts at: 0 1442 1677 Size: 1442 235 StyI C'CwwG_G Cuts at: 0 767 929 1677 Size: 767 162 748 Fragments arranged by size: 767 748 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 190 769 1521 1677 Size: 190 579 752 156 Fragments arranged by size: 752 579 190 156 TatI w'GTAC_w Cuts at: 0 84 1677 Size: 84 1593 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 1677 Size: 269 32 1376 Fragments arranged by size: 1376 269 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 276 1384 1677 Size: 276 1108 293 Fragments arranged by size: 1108 293 276 XmaI C'CCGG_G Cuts at: 0 374 1677 Size: 374 1303 XmnI GAAnn'nnTTC Cuts at: 0 70 662 860 1677 Size: 70 592 198 817 Fragments arranged by size: 817 592 198 70 Enzymes that do cut: AarI AclI AcuI AflIII AleI AlfI AloI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I HaeII MmeI MscI MslI MspA1I NcoI NspI PciI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI SexAI SfcI SmaI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII BbeI BbvCI BciVI BfrBI BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I ClaI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI