################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BstAPI BstBI CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 925 GTATAC GT^[AC][GT]AC AcyI 243 GACGCC G[GA]^CG[CT]C AlwNI 91 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 250 CCTCAGG CC^T[ACGT]AGG Aor51HI 374 AGCGCT AGC^GCT AspHI 427 GTGCTC G[AT]GC[AT]^C AspI 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 392 GGATCC G^GATCC BanII 195 GGGCTC G[GA]GC[CT]^C BbsI 600 GAAGAC GAAGAC(2/6) BclI 98 TGATCA T^GATCA BglI 688 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 600 GAAGAC GAAGAC(2/6) BsaAI 238 TACGTG [CT]AC^GT[GA] BsaHI 243 GACGCC G[GA]^CG[CT]C BsaI 31 GAGACC GGTCTC(1/5) BsiHKAI 427 GTGCTC G[AT]GC[AT]^C BsmI 933 GAATGC GAATGC(1/-1) BspHI 491 TCATGA T^CATGA BspMI 604 ACCTGC ACCTGC(4/8) BssHII 697 GCGCGC G^CGCGC BssSI 133 CACGAG CACGAG(-5/-1) Bst1107I 925 GTATAC GTA^TAC BstEII 180 GGTAACC G^GT[ACGT]ACC BstPI 180 GGTAACC G^GT[ACGT]ACC BstZ17I 925 GTATAC GTA^TAC Bsu36I 250 CCTCAGG CC^T[ACGT]AGG DrdI 33 GACCACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 461 CCACGG C^C[GA][CT]GG EaeI 402 TGGCCG [CT]^GGCC[GA] Eco47III 374 AGCGCT AGC^GCT Eco81I 250 CCTCAGG CC^T[ACGT]AGG EcoNI 888 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 180 GGTAACC G^GT[ACGT]ACC EcoT14I 185 CCAAGG C^C[AT][AT]GG FbaI 98 TGATCA T^GATCA HaeII 374 AGCGCT [GA]GCGC^[CT] HgaI 243 GACGC GACGC(5/10) Hin1I 243 GACGCC G[GA]^CG[CT]C MseI 722 TTAA T^TAA PflFI 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 626 AGGACCC [GA]G^G[AT]CC[CT] RcaI 491 TCATGA T^CATGA SapI 54 GAAGAGC GCTCTTC(1/4) ScaI 851 AGTACT AGT^ACT SexAI 146 ACCAGGT A^CC[AT]GGT SfcI 417 CTACAG C^T[GA][CT]AG SmlI 1039 CTCAAG C^T[CT][GA]AG StuI 752 AGGCCT AGG^CCT StyI 185 CCAAGG C^C[AT][AT]GG Tru9I 722 TTAA T^TAA Tth111I 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 698 CGCG CG^CG 1104 CGCG AccIII 199 TCCGGA T^CCGGA 431 TCCGGA BanI 256 GGCACC G^G[CT][GA]CC 455 GGTGCC BbvI 815 GCAGC GCAGC(8/12) 407 GCTGC BcgI 719 GCATTAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 905 GCATCCGCTTCG BcnI 618 CCCGG CC^[GC]GG 788 CCCGG BsaWI 199 TCCGGA [AT]^CCGG[AT] 431 TCCGGA BseAI 199 TCCGGA T^CCGGA 431 TCCGGA BseRI 331 GAGGAG GAGGAG(10/8) 1075 GAGGAG BsmAI 31 GAGAC GTCTC(1/5) 1087 GAGAC BspEI 199 TCCGGA T^CCGGA 431 TCCGGA BstUI 698 CGCG CG^CG 1104 CGCG BstYI 392 GGATCC [GA]^GATC[CT] 443 AGATCC DraII 626 AGGACCC [GA]G^G[ACGT]CC[CT] 1171 GGGGCCT EarI 547 CTCTTC CTCTTC(1/4) 54 GAAGAG EcoO109I 626 AGGACCC [GA]G^G[ACGT]CC[CT] 1171 GGGGCCT Ksp632I 547 CTCTTC CTCTTC(1/4) 54 GAAGAG MflI 392 GGATCC [GA]^GATC[CT] 443 AGATCC MroI 199 TCCGGA T^CCGGA 431 TCCGGA MvnI 698 CGCG CG^CG 1104 CGCG MwoI 688 GCCAAACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 731 GCTGGGTGTGC NciI 618 CCCGG CC^[GC]GG 788 CCCGG TaqI 794 TCGA T^CGA 914 TCGA TfiI 314 GATTC G^A[AT]TC 1165 GATTC TseI 407 GCTGC G^C[AT]GC 815 GCAGC Tsp45I 241 GTGAC ^GT[GC]AC 1016 GTGAC TthHB8I 794 TCGA T^CGA 914 TCGA XcmI 35 CCACCAAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 212 CCAACAGAGTCATGG XhoII 392 GGATCC [GA]^GATC[CT] 443 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TspRI