(Circular) (Six-base) MAPSORT of: pFN21AE2795.seq Check: 3440 from: 1 to: 5896 pFN21AE2795 5896 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2931 3239 1937 Size: 994 308 4594 Fragments arranged by size: 4594 994 308 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2827 2918 3959 5343 5611 1632 Size: 1195 91 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1195 1041 268 91 AfeI AGC'GCT Cuts at: 2381 2381 Size: 5896 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 2471 1166 Size: 1305 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2706 1184 Size: 1522 4374 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 5864 AlwNI CAG_nnn'CTG Cuts at: 2101 3639 5211 2101 Size: 1538 1572 2786 Fragments arranged by size: 2786 1572 1538 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 3205 3431 3583 5692 1493 Size: 1712 226 152 2109 1697 Fragments arranged by size: 2109 1712 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 2397 3226 3593 2397 Size: 829 367 4700 Fragments arranged by size: 4700 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2460 3217 4672 618 Size: 325 217 627 474 199 757 1455 1842 Fragments arranged by size: 1842 1455 757 627 474 325 217 199 BanII G_rGCy'C Cuts at: 1391 2204 3215 1391 Size: 813 1011 4072 Fragments arranged by size: 4072 1011 813 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2612 928 Size: 971 713 4212 Fragments arranged by size: 4212 971 713 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2713 2747 2899 2933 4087 4121 1903 Size: 34 776 34 152 34 1154 34 3678 Fragments arranged by size: 3678 1154 776 152 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 2103 1548 Size: 555 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2699 4507 136 Size: 107 122 71 631 605 1027 1808 1525 Fragments arranged by size: 1808 1525 1027 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 2264 2465 3957 5113 1790 Size: 474 201 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 474 201 BmgBI CAC'GTC Cuts at: 1318 2665 2923 1318 Size: 1347 258 4291 Fragments arranged by size: 4291 1347 258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3119 3627 4585 Size: 416 53 83 399 24 1702 508 958 Cuts at: 4585 442 Size: 1753 Fragments arranged by size: 1753 1702 958 508 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4556 1239 Size: 3317 2579 Bpu10I CC'TnA_GC Cuts at: 2376 2376 Size: 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3029 4021 4886 5184 3029 Size: 992 865 298 3741 Fragments arranged by size: 3741 992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 4559 882 Size: 617 531 2529 2219 Fragments arranged by size: 2529 2219 617 531 BsaAI yAC'GTr Cuts at: 493 1204 2245 3687 493 Size: 711 1041 1442 2702 Fragments arranged by size: 2702 1442 1041 711 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2249 3703 4085 4720 Size: 53 83 186 936 716 1454 382 635 Cuts at: 4720 275 Size: 1451 Fragments arranged by size: 1454 1451 936 716 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2204 2436 4329 4727 5001 5148 5559 2204 Size: 232 1893 398 274 147 411 2541 Fragments arranged by size: 2541 1893 411 398 274 232 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3146 3176 1178 Size: 30 165 30 1743 30 3898 Fragments arranged by size: 3898 1743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 3095 1186 Size: 1165 744 3987 Fragments arranged by size: 3987 1165 744 BseYI C'CCAG_C Cuts at: 2736 3134 5099 5595 2736 Size: 398 1965 496 3037 Fragments arranged by size: 3037 1965 496 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 3957 4042 5113 2436 Size: 1521 85 1071 3219 Fragments arranged by size: 3219 1521 1071 85 BsmI GAATG_Cn' Cuts at: 2944 3407 3500 2944 Size: 463 93 5340 Fragments arranged by size: 5340 463 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2264 2436 2465 3215 3957 4042 Size: 399 414 60 172 29 750 742 85 Cuts at: 4042 5113 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 750 742 414 399 172 85 60 29 BspEI T'CCGG_A Cuts at: 2204 2436 5559 2204 Size: 232 3123 2541 Fragments arranged by size: 3123 2541 232 BspHI T'CATG_A Cuts at: 2496 3785 5515 2496 Size: 1289 1730 2877 Fragments arranged by size: 2877 1730 1289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2618 3251 844 Size: 1774 633 3489 Fragments arranged by size: 3489 1774 633 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4391 4565 65 Size: 1183 3143 174 1396 Fragments arranged by size: 3143 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 2702 1925 Size: 777 5119 BssSI C'ACGA_G Cuts at: 2138 3956 4968 2138 Size: 1818 1012 3066 Fragments arranged by size: 3066 1818 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 2185 1184 Size: 1001 4895 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2473 2500 2050 Size: 423 27 5446 Fragments arranged by size: 5446 423 27 BstYI r'GATC_y Cuts at: 1972 2397 2448 3226 3593 3978 3995 5447 5533 Size: 425 51 778 367 385 17 1452 86 Cuts at: 5533 5545 5891 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 778 425 385 367 346 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2932 2932 Size: 5896 Bsu36I CC'TnA_GG Cuts at: 2256 2256 Size: 5896 BtgI C'CryG_G Cuts at: 513 1065 2466 513 Size: 552 1401 3943 Fragments arranged by size: 3943 1401 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3412 4206 4226 2884 Size: 528 794 20 4554 Fragments arranged by size: 4554 794 528 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2044 4903 809 Size: 507 728 2859 1802 Fragments arranged by size: 2859 1802 728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2407 3343 4232 Size: 54 1425 50 275 18 577 936 889 Cuts at: 4232 5861 8 Size: 1629 43 Fragments arranged by size: 1629 1425 936 889 577 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2558 3826 1353 Size: 701 504 1268 3423 Fragments arranged by size: 3423 1268 701 504 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2827 2918 3959 4556 5343 5611 1239 Size: 393 1195 91 1041 597 787 268 1524 Fragments arranged by size: 1524 1195 1041 787 597 393 268 91 EcoNI CCTnn'n_nnAGG Cuts at: 2897 2897 Size: 5896 EcoO109I rG'GnC_Cy Cuts at: 2632 3177 2632 Size: 545 5351 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 2383 5043 1678 Size: 705 2660 2531 Fragments arranged by size: 2660 2531 705 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 3146 3178 4537 Size: 32 135 32 667 32 875 32 1359 Cuts at: 4537 4569 4611 4643 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 875 667 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2043 5010 5194 1305 Size: 738 2967 184 2007 Fragments arranged by size: 2967 2007 738 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4074 Fragments arranged by size: 4074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2288 2471 2498 3854 4213 4372 Size: 648 1065 57 183 27 1356 359 159 Cuts at: 4372 518 Size: 2042 Fragments arranged by size: 2042 1356 1065 648 359 183 159 57 27 MspA1I CmG'CkG Cuts at: 1606 2349 2412 3152 3312 3989 5137 5382 1606 Size: 743 63 740 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 743 740 677 245 160 63 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5344 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 3254 4799 1132 Size: 97 2025 1545 2229 Fragments arranged by size: 2229 2025 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5896 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2443 3965 3997 5478 5510 2411 Size: 32 1522 32 1481 32 2797 Fragments arranged by size: 2797 1522 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 5896 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 3248 830 Size: 2418 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5896 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 2858 4144 5856 2858 Size: 1286 1712 2898 Fragments arranged by size: 2898 1712 1286 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 5896 SfcI C'TryA_G Cuts at: 826 1045 2422 3244 4379 5060 5251 826 Size: 219 1377 822 1135 681 191 1471 Fragments arranged by size: 1471 1377 1135 822 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3044 4000 4901 5163 820 Size: 197 926 1101 956 901 262 1553 Fragments arranged by size: 1553 1101 956 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 3254 1132 Size: 2122 3774 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 2759 1517 Size: 1242 4654 StyI C'CwwG_G Cuts at: 513 1065 2190 3335 513 Size: 552 1125 1145 3074 Fragments arranged by size: 3074 1145 1125 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 2728 3923 3940 4093 4278 4723 Size: 1255 677 471 1195 17 153 185 445 Cuts at: 4723 325 Size: 1498 Fragments arranged by size: 1498 1255 1195 677 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 3060 4142 Size: 274 80 33 51 1443 879 204 1082 Cuts at: 4142 5854 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1082 879 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5856 5888 5856 Size: 32 5864 Tth111I GACn'n_nGTC Cuts at: 1316 2666 1316 Size: 1350 4546 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2224 2047 Size: 177 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI