################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AocI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRII EcoRV EcoT14I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 383 CCGC CCGC(-3/-1) AcsI 3 GAATTC [GA]^AATT[CT] AgeI 362 ACCGGT A^CCGGT Aor51HI 573 AGCGCT AGC^GCT ApaI 586 GGGCCC GGGCC^C ApoI 3 GAATTC [GA]^AATT[CT] BbsI 22 GTCTTC GAAGAC(2/6) BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BcnI 72 CCCGG CC^[GC]GG BfaI 571 CTAG C^TAG BpmI 345 CTCCAG CTGGAG(16/14) BpuAI 22 GTCTTC GAAGAC(2/6) BsaWI 362 ACCGGT [AT]^CCGG[AT] BseRI 265 GAGGAG GAGGAG(10/8) BsgI 547 CTGCAC GTGCAG(16/14) BspMI 40 ACCTGC ACCTGC(4/8) BsrFI 362 ACCGGT [GA]^CCGG[CT] BssKI 72 CCCGG ^CC[ACGT]GG BstEII 75 GGTCACC G^GT[ACGT]ACC BstF5I 135 CATCC GGATG(2/0) BstPI 75 GGTCACC G^GT[ACGT]ACC CfoI 574 GCGC GCG^C Cfr10I 362 ACCGGT [GA]^CCGG[CT] Eco47III 573 AGCGCT AGC^GCT EcoO65I 75 GGTCACC G^GT[ACGT]ACC EcoRI 3 GAATTC G^AATTC EcoT22I 162 ATGCAT ATGCA^T FauI 382 CCCGC CCCGC(4/6) FokI 135 CATCC GGATG(9/13) HaeII 573 AGCGCT [GA]GCGC^[CT] HhaI 574 GCGC GCG^C HinP1I 574 GCGC G^CGC HindIII 449 AAGCTT A^AGCTT MaeI 571 CTAG C^TAG MaeIII 76 GTCAC ^GT[ACGT]AC MseI 421 TTAA T^TAA MslI 48 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 383 CCGCTG C[AC]G^C[GT]G NciI 72 CCCGG CC^[GC]GG NsiI 162 ATGCAT ATGCA^T NspI 551 ACATGC [GA]CATG^[CT] PflMI 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 362 ACCGGT A^CCGGT PleI 535 GAGTC GAGTC(4/5) SacI 258 GAGCTC GAGCT^C ScrFI 72 CCCGG CC^[ACGT]GG TaqI 204 TCGA T^CGA Tru9I 421 TTAA T^TAA Tsp45I 76 GTCAC ^GT[GC]AC TthHB8I 204 TCGA T^CGA Van91I 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 300 GAAGATGTTC AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC BanII 258 GAGCTC G[GA]GC[CT]^C 586 GGGCCC BsmFI 605 GGGAC GGGAC(10/14) 380 GTCCC BsmI 160 GAATGC GAATGC(1/-1) 281 GCATTC Cac8I 104 GCAGGC GC[ACGT]^[ACGT]GC 326 GCCTGC DraII 106 AGGCCCT [GA]G^G[ACGT]CC[CT] 585 AGGGCCC EcoO109I 106 AGGCCCT [GA]G^G[ACGT]CC[CT] 585 AGGGCCC HaeIII 107 GGCC GG^CC 587 GGCC HapII 73 CCGG C^CGG 363 CCGG HpaII 73 CCGG C^CGG 363 CCGG HphI 38 TCACC GGTGA(8/7) 77 TCACC MspI 73 CCGG C^CGG 363 CCGG NlaIII 54 CATG CATG^ 552 CATG NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC 586 GGGCCC PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 300 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AlwNI AspHI BbvI BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI Cfr13I DdeI Fnu4HI HinfI ItaI MboII MnlI MwoI Sau96I TfiI TseI Tsp509I TspRI