(Circular) (Six-base) MAPSORT of: pFN21AB0932.seq Check: 4687 from: 1 to: 5329 pFN21AB0932 5329 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 2054 Size: 5329 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3139 278 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2672 1937 Size: 735 4594 Acc65I G'GTAC_C Cuts at: 2650 2650 Size: 5329 AclI AA'CG_TT Cuts at: 1563 3456 3829 1563 Size: 1893 373 3063 Fragments arranged by size: 3063 1893 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3392 4776 5044 1632 Size: 1760 1384 268 1917 Fragments arranged by size: 1917 1760 1384 268 AfeI AGC'GCT Cuts at: 2580 2580 Size: 5329 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5132 AflIII A'CryG_T Cuts at: 1203 4228 5205 1203 Size: 3025 977 1327 Fragments arranged by size: 3025 1327 977 AgeI A'CCGG_T Cuts at: 2367 4160 2367 Size: 1793 3536 AhdI GACnn_n'nnGTC Cuts at: 4058 4058 Size: 5329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5329 AlwNI CAG_nnn'CTG Cuts at: 2115 2250 2382 2505 2569 3072 4644 2115 Size: 135 132 123 64 503 1572 2800 Fragments arranged by size: 2800 1572 503 135 132 123 64 ApaI G_GGCC'C Cuts at: 2595 2595 Size: 5329 ApaLI G'TGCA_C Cuts at: 3386 4542 3386 Size: 1156 4173 ApoI r'AATT_y Cuts at: 1493 2008 2638 2864 3016 5125 1493 Size: 515 630 226 152 2109 1697 Fragments arranged by size: 2109 1697 630 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 3883 160 Size: 872 2851 1606 Fragments arranged by size: 2851 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 AvaI C'yCGr_G Cuts at: 1870 1943 2654 1870 Size: 73 711 4545 Fragments arranged by size: 4545 711 73 BamHI G'GATC_C Cuts at: 2659 3026 2659 Size: 367 4962 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2650 4105 618 Size: 325 217 627 863 1455 1842 Fragments arranged by size: 1842 1455 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2267 2595 2648 1391 Size: 876 328 53 4072 Fragments arranged by size: 4072 876 328 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 928 Size: 971 121 4237 Fragments arranged by size: 4237 971 121 BbvCI CC'TCA_GC Cuts at: 2403 2403 Size: 5329 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3520 3554 1903 Size: 34 1583 34 3678 Fragments arranged by size: 3678 1583 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3221 4431 3221 Size: 1210 4119 BclI T'GATC_A Cuts at: 1548 1548 Size: 5329 BfrBI ATG'CAT Cuts at: 2169 2169 Size: 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3940 136 Size: 107 122 71 631 605 2268 1525 Fragments arranged by size: 2268 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5324 5324 Size: 5329 BlpI GC'TnA_GC Cuts at: 2746 2746 Size: 5329 Bme1580I G_kGCm'C Cuts at: 1790 2100 2595 3390 4546 1790 Size: 310 495 795 1156 2573 Fragments arranged by size: 2573 1156 795 495 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2422 3060 4018 Size: 416 53 83 399 24 1005 638 958 Cuts at: 4018 442 Size: 1753 Fragments arranged by size: 1753 1005 958 638 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 3989 1239 Size: 1096 1654 2579 Fragments arranged by size: 2579 1654 1096 Bpu10I CC'TnA_GC Cuts at: 2261 2403 2582 2261 Size: 142 179 5008 Fragments arranged by size: 5008 179 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3454 4319 4617 3454 Size: 865 298 4166 Fragments arranged by size: 4166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3992 882 Size: 617 2493 2219 Fragments arranged by size: 2493 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3120 493 Size: 711 1916 2702 Fragments arranged by size: 2702 1916 711 BsaBI GATnn'nnATC Cuts at: 5323 5323 Size: 5329 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3136 3518 4153 275 Size: 53 83 186 936 1603 382 635 1451 Fragments arranged by size: 1603 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2367 3762 4160 4434 4581 4992 2367 Size: 1395 398 274 147 411 2704 Fragments arranged by size: 2704 1395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 2354 2384 2408 Size: 30 165 30 861 30 60 30 24 Cuts at: 2408 2438 1178 Size: 30 4069 Fragments arranged by size: 4069 861 165 60 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 1186 Size: 1099 4230 BseYI C'CCAG_C Cuts at: 2562 4532 5028 2562 Size: 1970 496 2863 Fragments arranged by size: 2863 1970 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 1163 Size: 573 801 3955 Fragments arranged by size: 3955 801 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2779 3540 4568 1540 Size: 275 186 778 761 1028 2301 Fragments arranged by size: 2301 1028 778 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2351 2367 3390 3475 4546 2267 Size: 84 16 1023 85 1071 3050 Fragments arranged by size: 3050 1071 1023 85 84 16 BsmI GAATG_Cn' Cuts at: 2171 2286 2840 2933 2171 Size: 115 554 93 4567 Fragments arranged by size: 4567 554 115 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2267 2351 2367 2595 2648 3390 Size: 399 310 167 84 16 228 53 742 Cuts at: 3390 3475 4546 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 228 167 85 84 53 16 BspEI T'CCGG_A Cuts at: 4992 4992 Size: 5329 BspHI T'CATG_A Cuts at: 3218 4948 3218 Size: 1730 3599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2684 844 Size: 1210 630 3489 Fragments arranged by size: 3489 1210 630 BsrBI CCG'CTC Cuts at: 1394 1823 3216 1394 Size: 429 1393 3507 Fragments arranged by size: 3507 1393 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3824 3998 65 Size: 1183 2576 174 1396 Fragments arranged by size: 2576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 3973 4160 1700 Size: 110 557 1606 187 2869 Fragments arranged by size: 2869 1606 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5329 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5329 BssSI C'ACGA_G Cuts at: 3389 4401 3389 Size: 1012 4317 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5329 BstBI TT'CG_AA Cuts at: 4133 4133 Size: 5329 BstEII G'GTnAC_C Cuts at: 1184 2080 1184 Size: 896 4433 BstYI r'GATC_y Cuts at: 1972 2659 3026 3411 3428 4880 4966 4978 5324 Size: 687 367 385 17 1452 86 12 346 Cuts at: 5324 1972 Size: 1977 Fragments arranged by size: 1977 1452 687 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4777 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4590 Fragments arranged by size: 4590 683 56 BtsI GCAGTG_nn' Cuts at: 2845 3639 3659 2845 Size: 794 20 4515 Fragments arranged by size: 4515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5294 DraI TTT'AAA Cuts at: 2633 2633 Size: 5329 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4336 809 Size: 507 3020 1802 Fragments arranged by size: 3020 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2776 3665 5294 Size: 54 1425 50 275 18 946 889 1629 Cuts at: 5294 8 Size: 43 Fragments arranged by size: 1629 1425 946 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2776 1537 Size: 275 964 4090 Fragments arranged by size: 4090 964 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3259 1353 Size: 1906 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3916 4290 4436 387 Size: 1064 138 2327 374 146 1280 Fragments arranged by size: 2327 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2265 2265 Size: 5329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2335 3392 3989 4776 5044 1239 Size: 393 703 1057 597 787 268 1524 Fragments arranged by size: 1524 1057 787 703 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2112 2591 2112 Size: 479 4850 EcoRI G'AATT_C Cuts at: 2008 2638 3016 2008 Size: 630 378 4321 Fragments arranged by size: 4321 630 378 FspI TGC'GCA Cuts at: 3835 3835 Size: 5329 HaeII r_GCGC'y Cuts at: 1678 2582 4476 1678 Size: 904 1894 2531 Fragments arranged by size: 2531 1894 904 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3970 4002 4044 4076 1373 Size: 32 135 32 2398 32 42 32 2626 Fragments arranged by size: 2626 2398 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2673 2919 669 Size: 988 281 735 246 3079 Fragments arranged by size: 3079 988 735 281 246 HindIII A'AGCT_T Cuts at: 748 2454 748 Size: 1706 3623 HpaI GTT'AAC Cuts at: 2919 2919 Size: 5329 KpnI G_GTAC'C Cuts at: 2654 2654 Size: 5329 MfeI C'AATT_G Cuts at: 2928 2928 Size: 5329 MluI A'CGCG_T Cuts at: 5205 5205 Size: 5329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4443 4627 1305 Size: 3138 184 2007 Fragments arranged by size: 3138 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3507 Fragments arranged by size: 3507 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3287 3646 3805 518 Size: 648 891 1230 359 159 2042 Fragments arranged by size: 2042 1230 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2390 2745 3422 4570 4815 1606 Size: 784 355 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 677 355 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4777 NdeI CA'TA_TG Cuts at: 387 387 Size: 5329 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5329 NotI GC'GGCC_GC Cuts at: 2776 2776 Size: 5329 NsiI A_TGCA'T Cuts at: 2171 2171 Size: 5329 NspI r_CATG'y Cuts at: 1132 1229 2560 2687 4232 1132 Size: 97 1331 127 1545 2229 Fragments arranged by size: 2229 1545 1331 127 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5329 PciI A'CATG_T Cuts at: 4228 4228 Size: 5329 PflMI CCAn_nnn'nTGG Cuts at: 1108 2250 1108 Size: 1142 4187 PmeI GTTT'AAAC Cuts at: 2633 2633 Size: 5329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3430 4911 4943 3398 Size: 32 1481 32 3784 Fragments arranged by size: 3784 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5329 PsiI TTA'TAA Cuts at: 2899 2899 Size: 5329 PspOMI G'GGCC_C Cuts at: 2591 2591 Size: 5329 PstI C_TGCA'G Cuts at: 830 2681 830 Size: 1851 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5329 SacI G_AGCT'C Cuts at: 2267 2267 Size: 5329 SalI G'TCGA_C Cuts at: 1936 2671 1936 Size: 735 4594 SbfI CC_TGCA'GG Cuts at: 2681 2681 Size: 5329 ScaI AGT'ACT Cuts at: 3577 5289 3577 Size: 1712 3617 SfcI C'TryA_G Cuts at: 826 1045 2677 3812 4493 4684 826 Size: 219 1632 1135 681 191 1471 Fragments arranged by size: 1632 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5329 SmaI CCC'GGG Cuts at: 1872 2656 1872 Size: 784 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3433 4334 4596 820 Size: 197 926 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3120 493 Size: 2627 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5329 SphI G_CATG'C Cuts at: 1132 2687 1132 Size: 1555 3774 SspI AAT'ATT Cuts at: 5 52 3253 5 Size: 47 3201 2081 Fragments arranged by size: 3201 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5329 StyI C'CwwG_G Cuts at: 513 1065 2768 513 Size: 552 1703 3074 Fragments arranged by size: 3074 1703 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3356 3373 3526 3711 4156 325 Size: 1255 1776 17 153 185 445 1498 Fragments arranged by size: 1776 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3575 5287 96 Size: 274 80 33 51 1443 1598 1712 138 Fragments arranged by size: 1712 1598 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 5289 5321 2264 Size: 32 2993 32 2272 Fragments arranged by size: 2993 2272 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2078 2382 1316 Size: 762 304 4263 Fragments arranged by size: 4263 762 304 XbaI T'CTAG_A Cuts at: 2665 2665 Size: 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5329 XmaI C'CCGG_G Cuts at: 1870 2654 1870 Size: 784 4545 XmnI GAAnn'nnTTC Cuts at: 2066 2309 3458 2066 Size: 243 1149 3937 Fragments arranged by size: 3937 1149 243 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3137 276 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI