Restriction map for
pFN21AE4037
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbrPI BbsI BcgI BclI
BfrI BglI BglII BlpI Bpu1102I
BpuAI BsaBI BseAI BsgI BsiEI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MslI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 537 GTCTAC GT^[AC][GT]AC
AflIII 1301 ACATGT A^C[GA][CT]GT
Alw44I 154 GTGCAC G^TGCAC
AocI 1091 CCTTAGG CC^T[ACGT]AGG
ApaI 695 GGGCCC GGGCC^C
ApaLI 154 GTGCAC G^TGCAC
Asp700I 216 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1246 CTCGAG C^[CT]CG[GA]G
AvrII 690 CCTAGG C^CTAGG
BalI 1517 TGGCCA TGG^CCA
BbeI 88 GGCGCC GGCGC^C
BbvCI 228 GCTGAGG CCTCAGC(-5/-2)
BlnI 690 CCTAGG C^CTAGG
BpmI 841 CTCCAG CTGGAG(16/14)
BsaAI 117 CACGTA [CT]AC^GT[GA]
BsaI 1343 GGTCTC GGTCTC(1/5)
BsaWI 1214 ACCGGA [AT]^CCGG[AT]
BsiWI 119 CGTACG C^GTACG
BsmI 252 GAATGC GAATGC(1/-1)
BsoBI 1246 CTCGAG C^[CT]CG[GA]G
BspLU11I 1301 ACATGT A^CATGT
BsrDI 1464 CATTGC GCAATG(2/0)
BstAPI 775 GCAGCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 350 AGATCC [GA]^GATC[CT]
Bsu36I 1091 CCTTAGG CC^T[ACGT]AGG
DraIII 952 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 806 CCATGG C^C[GA][CT]GG
EaeI 1517 TGGCCA [CT]^GGCC[GA]
EarI 1383 CTCTTC CTCTTC(1/4)
Eco81I 1091 CCTTAGG CC^T[ACGT]AGG
EcoRI 1533 GAATTC G^AATTC
HaeII 88 GGCGCC [GA]GCGC^[CT]
HindIII 1101 AAGCTT A^AGCTT
KasI 88 GGCGCC G^GCGCC
Ksp632I 1383 CTCTTC CTCTTC(1/4)
MaeIII 1171 GTGAC ^GT[ACGT]AC
MflI 350 AGATCC [GA]^GATC[CT]
MluNI 1517 TGGCCA TGG^CCA
MscI 1517 TGGCCA TGG^CCA
NarI 88 GGCGCC GG^CGCC
NcoI 806 CCATGG C^CATGG
NspI 1301 ACATGT [GA]CATG^[CT]
PaeR7I 1246 CTCGAG C^TCGAG
PpuMI 517 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1351 CTGCAG CTGCA^G
SapI 1382 GCTCTTC GCTCTTC(1/4)
SfoI 88 GGCGCC GGC^GCC
Tsp45I 1171 GTGAC ^GT[GC]AC
XcmI 605 CCAAAGCCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1246 CTCGAG C^TCGAG
XhoII 350 AGATCC [GA]^GATC[CT]
XmnI 216 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 52 CGCG CG^CG
1262 CGCG
AcsI 1270 AAATTC [GA]^AATT[CT]
1533 GAATTC
AcyI 88 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
AlwI 351 GATCC GGATC(4/5)
1545 GGATC
ApoI 1270 AAATTC [GA]^AATT[CT]
1533 GAATTC
BciVI 1119 GGATAC GTATCC(6/5)
931 GTATCC
BcnI 149 CCGGG CC^[GC]GG
342 CCGGG
BsaHI 88 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
BseRI 799 GAGGAG GAGGAG(10/8)
1153 GAGGAG
BsmAI 946 GTCTC GTCTC(1/5)
1344 GTCTC
BsmFI 517 GGGAC GGGAC(10/14)
1400 GGGAC
BsrBI 159 CCGCTC CCGCTC(-3/-3)
733 GAGCGG
BstUI 52 CGCG CG^CG
1262 CGCG
BstXI 194 CCACAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
818 CCATGCGTCTGG
DraII 517 GGGACCC [GA]G^G[ACGT]CC[CT]
694 GGGGCCC
EcoO109I 517 GGGACCC [GA]G^G[ACGT]CC[CT]
694 GGGGCCC
EcoT14I 690 CCTAGG C^C[AT][AT]GG
806 CCATGG
Hin1I 88 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
MaeII 118 ACGT A^CGT
1445 ACGT
MseI 41 TTAA T^TAA
1475 TTAA
MvnI 52 CGCG CG^CG
1262 CGCG
NciI 149 CCGGG CC^[GC]GG
342 CCGGG
SmlI 1179 CTCAAG C^T[CT][GA]AG
1246 CTCGAG
StyI 690 CCTAGG C^C[AT][AT]GG
806 CCATGG
TaqI 80 TCGA T^CGA
1247 TCGA
Tru9I 41 TTAA T^TAA
1475 TTAA
Tsp509I 1271 AATT ^AATT
1534 AATT
TthHB8I 80 TCGA T^CGA
1247 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TfiI TseI TspRI