(Circular) (Six-base) MAPSORT of: pFN21AE4037.seq Check: 4220 from: 1 to: 6268 pFN21AE4037 6268 bp With 182 enzymes: SgfI * March 28, 2012 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2968 2968 Size: 6268 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2543 3611 1937 Size: 606 1068 4594 Fragments arranged by size: 4594 1068 606 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 2710 3375 4331 5715 5983 1632 Size: 870 208 665 956 1384 268 1917 Fragments arranged by size: 1917 1384 956 870 665 268 208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 3306 5167 6144 1203 Size: 2103 1861 977 1327 Fragments arranged by size: 2103 1861 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 2510 2887 3134 4011 5583 2510 Size: 377 247 877 1572 3195 Fragments arranged by size: 3195 1572 877 377 247 ApaI G_GGCC'C Cuts at: 2704 2704 Size: 6268 ApaLI G'TGCA_C Cuts at: 2159 4325 5481 2159 Size: 2166 1156 2946 Fragments arranged by size: 2946 2166 1156 ApoI r'AATT_y Cuts at: 1493 3275 3538 3577 3803 3955 6064 1493 Size: 1782 263 39 226 152 2109 1697 Fragments arranged by size: 2109 1782 1697 263 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3251 3593 1870 Size: 73 1308 342 4545 Fragments arranged by size: 4545 1308 342 73 AvrII C'CTAG_G Cuts at: 2695 2695 Size: 6268 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2600 2681 3589 5044 Size: 325 217 627 306 507 81 908 1455 Cuts at: 5044 618 Size: 1842 Fragments arranged by size: 1842 1455 908 627 507 325 306 217 81 BanII G_rGCy'C Cuts at: 1391 2704 2847 3245 3421 3587 1391 Size: 1313 143 398 176 166 4072 Fragments arranged by size: 4072 1313 398 176 166 143 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 6268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5297 BbvCI CC'TCA_GC Cuts at: 2234 2234 Size: 6268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2947 3118 4160 5370 2947 Size: 171 1042 1210 3845 Fragments arranged by size: 3845 1210 1042 171 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4879 136 Size: 107 122 71 631 605 337 2870 1525 Fragments arranged by size: 2870 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3685 3685 Size: 6268 Bme1580I G_kGCm'C Cuts at: 1790 2163 2704 4329 5485 1790 Size: 373 541 1625 1156 2573 Fragments arranged by size: 2573 1625 1156 541 373 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2993 3469 3999 Size: 416 53 83 399 24 1576 476 530 Cuts at: 3999 4957 442 Size: 958 1753 Fragments arranged by size: 1753 1576 958 530 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2831 4928 1239 Size: 1592 2097 2579 Fragments arranged by size: 2579 2097 1592 Bpu10I CC'TnA_GC Cuts at: 2234 2748 3244 2234 Size: 514 496 5258 Fragments arranged by size: 5258 514 496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3169 4393 5258 5556 3169 Size: 1224 865 298 3881 Fragments arranged by size: 3881 1224 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3354 4931 882 Size: 617 1855 1577 2219 Fragments arranged by size: 2219 1855 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 2124 4059 493 Size: 711 920 1935 2702 Fragments arranged by size: 2702 1935 920 711 BsaBI GATnn'nnATC Cuts at: 6262 6262 Size: 6268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2175 4075 4457 Size: 53 83 186 936 561 81 1900 382 Cuts at: 4457 5092 275 Size: 635 1451 Fragments arranged by size: 1900 1451 936 635 561 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 3219 4701 5099 5373 5520 5931 3219 Size: 1482 398 274 147 411 3556 Fragments arranged by size: 3556 1482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6043 Fragments arranged by size: 6043 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 3173 1186 Size: 1633 354 4281 Fragments arranged by size: 4281 1633 354 BseYI C'CCAG_C Cuts at: 2365 2526 2677 3120 3503 5471 5967 2365 Size: 161 151 443 383 1968 496 2666 Fragments arranged by size: 2666 1968 496 443 383 161 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5695 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 2847 3245 3421 4329 4414 5485 2163 Size: 684 398 176 908 85 1071 2946 Fragments arranged by size: 2946 1071 908 684 398 176 85 BsiWI C'GTAC_G Cuts at: 2124 2124 Size: 6268 BsmI GAATG_Cn' Cuts at: 2263 3779 3872 2263 Size: 1516 93 4659 Fragments arranged by size: 4659 1516 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2704 2847 3245 3421 3587 4329 Size: 399 373 541 143 398 176 166 742 Cuts at: 4329 4414 5485 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 398 373 176 166 143 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2601 2968 3623 844 Size: 1392 365 367 655 3489 Fragments arranged by size: 3489 1392 655 367 365 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2740 4155 1394 Size: 429 343 574 1415 3507 Fragments arranged by size: 3507 1415 574 429 343 BsrDI GCAATG_nn' Cuts at: 65 1248 3468 4763 4937 65 Size: 1183 2220 1295 174 1396 Fragments arranged by size: 2220 1396 1295 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 2786 794 Size: 1992 4276 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2830 2206 Size: 624 5644 BstYI r'GATC_y Cuts at: 1972 2355 3598 3965 4350 4367 5819 5905 5917 Size: 383 1243 367 385 17 1452 86 12 Cuts at: 5917 6263 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1243 385 383 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3097 3097 Size: 6268 BtgI C'CryG_G Cuts at: 513 1065 2004 2811 513 Size: 552 939 807 3970 Fragments arranged by size: 3970 939 807 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 2560 3382 3784 4578 4598 2560 Size: 822 402 794 20 4230 Fragments arranged by size: 4230 822 794 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DraIII CAC_nnn'GTG Cuts at: 2962 2962 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3522 3715 4604 Size: 54 1425 50 275 18 1692 193 889 Cuts at: 4604 6233 8 Size: 1629 43 Fragments arranged by size: 1692 1629 1425 889 275 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3394 4198 1353 Size: 2041 804 3423 Fragments arranged by size: 3423 2041 804 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2816 4855 5229 5375 387 Size: 1064 138 438 789 2039 374 146 1280 Fragments arranged by size: 2039 1280 1064 789 438 374 146 138 EcoICRI GAG'CTC Cuts at: 2845 3243 3419 2845 Size: 398 176 5694 Fragments arranged by size: 5694 398 176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 2710 2831 3375 4331 4928 5715 Size: 393 870 208 121 544 956 597 787 Cuts at: 5715 5983 1239 Size: 268 1524 Fragments arranged by size: 1524 956 870 787 597 544 393 268 208 121 EcoO109I rG'GnC_Cy Cuts at: 2523 2700 2523 Size: 177 6091 EcoRI G'AATT_C Cuts at: 3538 3577 3955 3538 Size: 39 378 5851 Fragments arranged by size: 5851 378 39 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2097 5415 1678 Size: 419 3318 2531 Fragments arranged by size: 3318 2531 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3332 3364 4909 4941 4983 Size: 32 135 32 1760 32 1545 32 42 Cuts at: 4983 5015 1373 Size: 32 2626 Fragments arranged by size: 2626 1760 1545 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3612 3858 669 Size: 988 281 1674 246 3079 Fragments arranged by size: 3079 1674 988 281 246 HindIII A'AGCT_T Cuts at: 748 3106 748 Size: 2358 3910 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KasI G'GCGC_C Cuts at: 2093 2093 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2090 2187 2238 5382 5566 1305 Size: 785 97 51 3144 184 2007 Fragments arranged by size: 3144 2007 785 184 97 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 3524 10 Size: 54 1425 343 1692 2754 Fragments arranged by size: 2754 1692 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4226 4585 4744 518 Size: 648 3060 359 159 2042 Fragments arranged by size: 3060 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 2365 3028 3120 3464 3684 4361 5509 Size: 627 132 663 92 344 220 677 1148 Cuts at: 5509 5754 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 663 627 344 245 220 132 92 NarI GG'CG_CC Cuts at: 2094 2094 Size: 6268 NcoI C'CATG_G Cuts at: 513 1065 2004 2811 513 Size: 552 939 807 3970 Fragments arranged by size: 3970 939 807 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 3310 3626 5171 1132 Size: 97 2081 316 1545 2229 Fragments arranged by size: 2229 2081 1545 316 97 PasI CC'CwG_GG Cuts at: 1348 2170 1348 Size: 822 5446 PciI A'CATG_T Cuts at: 3306 5167 3306 Size: 1861 4407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2523 2523 Size: 6268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PspOMI G'GGCC_C Cuts at: 2700 2700 Size: 6268 PspXI vC'TCGA_Gb Cuts at: 3251 3251 Size: 6268 PstI C_TGCA'G Cuts at: 830 3360 3620 830 Size: 2530 260 3478 Fragments arranged by size: 3478 2530 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 PvuII CAG'CTG Cuts at: 2233 2365 3028 2233 Size: 132 663 5473 Fragments arranged by size: 5473 663 132 SacI G_AGCT'C Cuts at: 2847 3245 3421 2847 Size: 398 176 5694 Fragments arranged by size: 5694 398 176 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SanDI GG'GwC_CC Cuts at: 2523 2523 Size: 6268 SapI GCTCTTCn'nnn_ Cuts at: 3394 3394 Size: 6268 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2544 2879 3356 3459 3616 4751 5432 Size: 219 1499 335 477 103 157 1135 681 Cuts at: 5432 5623 826 Size: 191 1471 Fragments arranged by size: 1499 1471 1135 681 477 335 219 191 157 103 SfoI GGC'GCC Cuts at: 2095 2095 Size: 6268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3184 3251 4372 5273 5535 820 Size: 197 926 1241 67 1121 901 262 1553 Fragments arranged by size: 1553 1241 1121 926 901 262 197 67 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 2078 2694 3249 1517 Size: 561 616 555 4536 Fragments arranged by size: 4536 616 561 555 StyI C'CwwG_G Cuts at: 513 1065 2004 2695 2811 3707 513 Size: 552 939 691 116 896 3074 Fragments arranged by size: 3074 939 896 691 552 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2623 2669 4295 4312 4465 4650 Size: 1255 462 581 46 1626 17 153 185 Cuts at: 4650 5095 325 Size: 445 1498 Fragments arranged by size: 1626 1498 1255 581 462 445 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3075 4514 6226 Size: 274 80 33 51 1443 1098 1439 1712 Cuts at: 6226 96 Size: 138 Fragments arranged by size: 1712 1443 1439 1098 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6268 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 2617 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 3251 1943 Size: 1308 4960 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 2225 4397 2225 Size: 2172 4096 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BplI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI