(Linear) (Six-base) MAPSORT of: KIEE4037.seq Check: 1549 from: 1 to: 1563 KIEE4037.orf With 182 enzymes: SgfI * March 22, 2012 17:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 963 1563 Size: 963 600 AccI GT'mk_AC Cuts at: 0 538 1563 Size: 538 1025 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 497 705 1370 1563 Size: 497 208 665 193 Fragments arranged by size: 665 497 208 193 AflIII A'CryG_T Cuts at: 0 1301 1563 Size: 1301 262 AlwNI CAG_nnn'CTG Cuts at: 0 505 882 1129 1563 Size: 505 377 247 434 Fragments arranged by size: 505 434 377 247 ApaI G_GGCC'C Cuts at: 0 699 1563 Size: 699 864 ApaLI G'TGCA_C Cuts at: 0 154 1563 Size: 154 1409 ApoI r'AATT_y Cuts at: 0 1270 1533 1563 Size: 1270 263 30 Fragments arranged by size: 1270 263 30 AvaI C'yCGr_G Cuts at: 0 1246 1563 Size: 1246 317 AvrII C'CTAG_G Cuts at: 0 690 1563 Size: 690 873 BanI G'GyrC_C Cuts at: 0 88 595 676 1563 Size: 88 507 81 887 Fragments arranged by size: 887 507 88 81 BanII G_rGCy'C Cuts at: 0 699 842 1240 1416 1563 Size: 699 143 398 176 147 Fragments arranged by size: 699 398 176 147 143 BbeI G_GCGC'C Cuts at: 0 92 1563 Size: 92 1471 BbvCI CC'TCA_GC Cuts at: 0 229 1563 Size: 229 1334 BciVI GTATCCnnnnn_n' Cuts at: 0 942 1113 1563 Size: 942 171 450 Fragments arranged by size: 942 450 171 Bme1580I G_kGCm'C Cuts at: 0 158 699 1563 Size: 158 541 864 Fragments arranged by size: 864 541 158 BmrI ACTGGGnnnn_n' Cuts at: 0 988 1464 1563 Size: 988 476 99 Fragments arranged by size: 988 476 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 826 1563 Size: 826 737 Bpu10I CC'TnA_GC Cuts at: 0 229 743 1239 1563 Size: 229 514 496 324 Fragments arranged by size: 514 496 324 229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1164 1563 Size: 1164 399 BsaI GGTCTCn'nnnn_ Cuts at: 0 1349 1563 Size: 1349 214 BsaAI yAC'GTr Cuts at: 0 119 1563 Size: 119 1444 BsaHI Gr'CG_yC Cuts at: 0 89 170 1563 Size: 89 81 1393 Fragments arranged by size: 1393 89 81 BsaWI w'CCGG_w Cuts at: 0 1214 1563 Size: 1214 349 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 814 1168 1563 Size: 814 354 395 Fragments arranged by size: 814 395 354 BseYI C'CCAG_C Cuts at: 0 360 521 672 1115 1498 1563 Size: 360 161 151 443 383 65 Fragments arranged by size: 443 383 360 161 151 65 BsiHKAI G_wGCw'C Cuts at: 0 158 842 1240 1416 1563 Size: 158 684 398 176 147 Fragments arranged by size: 684 398 176 158 147 BsiWI C'GTAC_G Cuts at: 0 119 1563 Size: 119 1444 BsmI GAATG_Cn' Cuts at: 0 258 1563 Size: 258 1305 Bsp1286I G_dGCh'C Cuts at: 0 158 699 842 1240 1416 1563 Size: 158 541 143 398 176 147 Fragments arranged by size: 541 398 176 158 147 143 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 231 596 963 1563 Size: 231 365 367 600 Fragments arranged by size: 600 367 365 231 BsrBI CCG'CTC Cuts at: 0 161 735 1563 Size: 161 574 828 Fragments arranged by size: 828 574 161 BsrDI GCAATG_nn' Cuts at: 0 1463 1563 Size: 1463 100 BstAPI GCAn_nnn'nTGC Cuts at: 0 781 1563 Size: 781 782 BstXI CCAn_nnnn'nTGG Cuts at: 0 201 825 1563 Size: 201 624 738 Fragments arranged by size: 738 624 201 BstYI r'GATC_y Cuts at: 0 350 1563 Size: 350 1213 Bsu36I CC'TnA_GG Cuts at: 0 1092 1563 Size: 1092 471 BtgI C'CryG_G Cuts at: 0 806 1563 Size: 806 757 BtsI GCAGTG_nn' Cuts at: 0 555 1377 1563 Size: 555 822 186 Fragments arranged by size: 822 555 186 DraIII CAC_nnn'GTG Cuts at: 0 957 1563 Size: 957 606 EaeI y'GGCC_r Cuts at: 0 1517 1563 Size: 1517 46 EarI CTCTTCn'nnn_ Cuts at: 0 1389 1563 Size: 1389 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 811 1563 Size: 22 789 752 Fragments arranged by size: 789 752 22 EcoICRI GAG'CTC Cuts at: 0 840 1238 1414 1563 Size: 840 398 176 149 Fragments arranged by size: 840 398 176 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 497 705 826 1370 1563 Size: 497 208 121 544 193 Fragments arranged by size: 544 497 208 193 121 EcoO109I rG'GnC_Cy Cuts at: 0 518 695 1563 Size: 518 177 868 Fragments arranged by size: 868 518 177 EcoRI G'AATT_C Cuts at: 0 1533 1563 Size: 1533 30 HaeII r_GCGC'y Cuts at: 0 92 1563 Size: 92 1471 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1327 1359 1563 Size: 1327 32 204 Fragments arranged by size: 1327 204 32 HindIII A'AGCT_T Cuts at: 0 1101 1563 Size: 1101 462 KasI G'GCGC_C Cuts at: 0 88 1563 Size: 88 1475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 85 182 233 1563 Size: 85 97 51 1330 Fragments arranged by size: 1330 97 85 51 MscI TGG'CCA Cuts at: 0 1519 1563 Size: 1519 44 MspA1I CmG'CkG Cuts at: 0 228 360 1023 1115 1459 1563 Size: 228 132 663 92 344 104 Fragments arranged by size: 663 344 228 132 104 92 NarI GG'CG_CC Cuts at: 0 89 1563 Size: 89 1474 NcoI C'CATG_G Cuts at: 0 806 1563 Size: 806 757 NspI r_CATG'y Cuts at: 0 1305 1563 Size: 1305 258 PasI CC'CwG_GG Cuts at: 0 165 1563 Size: 165 1398 PciI A'CATG_T Cuts at: 0 1301 1563 Size: 1301 262 PpuMI rG'GwC_Cy Cuts at: 0 518 1563 Size: 518 1045 PspOMI G'GGCC_C Cuts at: 0 695 1563 Size: 695 868 PspXI vC'TCGA_Gb Cuts at: 0 1246 1563 Size: 1246 317 PstI C_TGCA'G Cuts at: 0 1355 1563 Size: 1355 208 PvuII CAG'CTG Cuts at: 0 228 360 1023 1563 Size: 228 132 663 540 Fragments arranged by size: 663 540 228 132 SacI G_AGCT'C Cuts at: 0 842 1240 1416 1563 Size: 842 398 176 147 Fragments arranged by size: 842 398 176 147 SanDI GG'GwC_CC Cuts at: 0 518 1563 Size: 518 1045 SapI GCTCTTCn'nnn_ Cuts at: 0 1389 1563 Size: 1389 174 SfcI C'TryA_G Cuts at: 0 539 874 1351 1454 1563 Size: 539 335 477 103 109 Fragments arranged by size: 539 477 335 109 103 SfoI GGC'GCC Cuts at: 0 90 1563 Size: 90 1473 SmlI C'TyrA_G Cuts at: 0 1179 1246 1563 Size: 1179 67 317 Fragments arranged by size: 1179 317 67 StuI AGG'CCT Cuts at: 0 73 689 1244 1563 Size: 73 616 555 319 Fragments arranged by size: 616 555 319 73 StyI C'CwwG_G Cuts at: 0 690 806 1563 Size: 690 116 757 Fragments arranged by size: 757 690 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 37 618 664 1563 Size: 37 581 46 899 Fragments arranged by size: 899 581 46 37 TatI w'GTAC_w Cuts at: 0 1070 1563 Size: 1070 493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 612 1563 Size: 612 951 XhoI C'TCGA_G Cuts at: 0 1246 1563 Size: 1246 317 XmnI GAAnn'nnTTC Cuts at: 0 220 1563 Size: 220 1343 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII KasI MmeI MscI MspA1I NarI NcoI NspI PasI PciI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI SfcI SfoI SmlI StuI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI BaeI BamHI BbsI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaBI BsaXI BsgI BsiEI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI ZraI