################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 824 GGCGCC G[GA]^CG[CT]C AspHI 569 GAGCTC G[AT]GC[AT]^C AspI 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 830 GGACC G^G[AT]CC BalI 353 TGGCCA TGG^CCA BanI 824 GGCGCC G^G[CT][GA]CC BanII 569 GAGCTC G[GA]GC[CT]^C BbeI 824 GGCGCC GGCGC^C BmyI 569 GAGCTC G[AGT]GC[ACT]^C BsaHI 824 GGCGCC G[GA]^CG[CT]C BseRI 610 GAGGAG GAGGAG(10/8) BsiHKAI 569 GAGCTC G[AT]GC[AT]^C Bsp1286I 569 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 668 TGTACA T^GTACA BsrDI 672 CATTGC GCAATG(2/0) BsrGI 668 TGTACA T^GTACA BstAPI 237 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 463 GGTGACC G^GT[ACGT]ACC BstPI 463 GGTGACC G^GT[ACGT]ACC DraII 791 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 62 CCACGG C^C[GA][CT]GG EarI 898 CTCTTC CTCTTC(1/4) EcoNI 192 CCTACGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 791 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 463 GGTGACC G^GT[ACGT]ACC HaeII 824 GGCGCC [GA]GCGC^[CT] Hin1I 824 GGCGCC G[GA]^CG[CT]C KasI 824 GGCGCC G^GCGCC Ksp632I 898 CTCTTC CTCTTC(1/4) MboII 899 TCTTC GAAGA(8/7) MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MslI 285 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 824 GGCGCC GG^CGCC PflFI 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 45 GACTC GAGTC(4/5) PstI 643 CTGCAG CTGCA^G SacI 569 GAGCTC GAGCT^C SfiI 57 GGCCGCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 824 GGCGCC GGC^GCC SspBI 668 TGTACA T^GTACA TaqI 114 TCGA T^CGA Tth111I 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 114 TCGA T^CGA XcmI 62 CCACGGCCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### BamHI 188 GGATCC G^GATCC 893 GGATCC BglI 58 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 972 GCCCACCTGGC BlpI 255 GCTGAGC GC^T[ACGT]AGC 696 GCTCAGC Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC 696 GCTCAGC BsaAI 40 TACGTG [CT]AC^GT[GA] 496 TACGTG BsaI 439 GAGACC GGTCTC(1/5) 649 GAGACC BsgI 550 CTGCAC GTGCAG(16/14) 688 CTGCAC BsrBI 955 CCGCTC CCGCTC(-3/-3) 808 GAGCGG BssSI 130 CACGAG CACGAG(-5/-1) 607 CACGAG BstYI 188 GGATCC [GA]^GATC[CT] 893 GGATCC CelII 255 GCTGAGC GC^T[ACGT]AGC 696 GCTCAGC CfoI 825 GCGC GCG^C 858 GCGC DpnII 189 GATC ^GATC 894 GATC EagI 56 CGGCCG C^GGCCG 679 CGGCCG EclXI 56 CGGCCG C^GGCCG 679 CGGCCG Eco52I 56 CGGCCG C^GGCCG 679 CGGCCG EcoT14I 302 CCAAGG C^C[AT][AT]GG 356 CCAAGG HhaI 825 GCGC GCG^C 858 GCGC HinP1I 825 GCGC G^CGC 858 GCGC MboI 189 GATC ^GATC 894 GATC MflI 188 GGATCC [GA]^GATC[CT] 893 GGATCC NdeII 189 GATC ^GATC 894 GATC PvuII 70 CAGCTG CAG^CTG 349 CAGCTG Sau3AI 189 GATC ^GATC 894 GATC SfaNI 372 GATGC GCATC(5/9) 603 GCATC SfcI 643 CTGCAG C^T[GA][CT]AG 996 CTACAG StyI 302 CCAAGG C^C[AT][AT]GG 356 CCAAGG TfiI 116 GATTC G^A[AT]TC 124 GATTC XhoII 188 GGATCC [GA]^GATC[CT] 893 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BcnI BpmI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflMI RsaI Sau96I ScrFI TseI Tsp45I TspRI Van91I