(Circular) (Six-base) MAPSORT of: pFN21AE6617.seq Check: 7494 from: 1 to: 5713 pFN21AE6617 5713 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 1937 Size: 1119 4594 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2083 3776 5160 5428 1632 Size: 451 1693 1384 268 1917 Fragments arranged by size: 1917 1693 1384 451 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 4612 5589 1203 Size: 3409 977 1327 Fragments arranged by size: 3409 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5713 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 5681 AlwNI CAG_nnn'CTG Cuts at: 2248 2568 2920 3456 5028 2248 Size: 320 352 536 1572 2933 Fragments arranged by size: 2933 1572 536 352 320 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 3022 3248 3400 5509 1493 Size: 1529 226 152 2109 1697 Fragments arranged by size: 2109 1697 1529 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BamHI G'GATC_C Cuts at: 2193 2898 3043 3410 2193 Size: 705 145 367 4496 Fragments arranged by size: 4496 705 367 145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2829 3034 4489 618 Size: 325 217 627 1042 205 1455 1842 Fragments arranged by size: 1842 1455 1042 627 325 217 205 BanII G_rGCy'C Cuts at: 1391 2578 3032 1391 Size: 1187 454 4072 Fragments arranged by size: 4072 1187 454 BbeI G_GCGC'C Cuts at: 2833 2833 Size: 5713 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 2983 4324 Size: 107 122 71 631 605 397 914 1341 Cuts at: 4324 136 Size: 1525 Fragments arranged by size: 1525 1341 914 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 2261 2702 3130 2261 Size: 441 428 4844 Fragments arranged by size: 4844 441 428 Bme1580I G_kGCm'C Cuts at: 1790 3774 4930 1790 Size: 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 BmgBI CAC'GTC Cuts at: 1318 2764 2932 1318 Size: 1446 168 4099 Fragments arranged by size: 4099 1446 168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2578 3444 4402 Size: 416 53 83 399 24 1161 866 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1161 958 866 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2291 2393 2522 2545 2897 4373 1239 Size: 860 192 102 129 23 352 1476 2579 Fragments arranged by size: 2579 1476 860 352 192 129 102 23 Bpu10I CC'TnA_GC Cuts at: 2774 2774 Size: 5713 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 2648 4376 882 Size: 617 939 210 1728 2219 Fragments arranged by size: 2219 1728 939 617 210 BsaAI yAC'GTr Cuts at: 493 1204 2047 2503 3504 493 Size: 711 843 456 1001 2702 Fragments arranged by size: 2702 1001 843 711 456 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2830 3520 3902 4537 Size: 53 83 186 936 1297 690 382 635 Cuts at: 4537 275 Size: 1451 Fragments arranged by size: 1451 1297 936 690 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 1178 Size: 30 165 30 1467 30 3991 Fragments arranged by size: 3991 1467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 1186 Size: 1444 4269 BseYI C'CCAG_C Cuts at: 2073 2238 2580 4916 5412 2073 Size: 165 342 2336 496 2374 Fragments arranged by size: 2374 2336 496 342 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2540 2678 1163 Size: 573 804 138 4198 Fragments arranged by size: 4198 804 573 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 2687 2881 3163 3924 4952 Size: 275 186 63 623 194 282 761 1028 Cuts at: 4952 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 623 282 275 194 186 63 BsiHKAI G_wGCw'C Cuts at: 2578 3774 3859 4930 2578 Size: 1196 85 1071 3361 Fragments arranged by size: 3361 1196 1071 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2578 3032 3774 3859 4930 1391 Size: 399 788 454 742 85 1071 2174 Fragments arranged by size: 2174 1071 788 742 454 399 85 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2105 2115 2178 2865 3068 844 Size: 1261 10 63 687 203 3489 Fragments arranged by size: 3489 1261 687 203 63 10 BsrBI CCG'CTC Cuts at: 1394 1823 2815 2962 3600 1394 Size: 429 992 147 638 3507 Fragments arranged by size: 3507 992 638 429 147 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 4208 4382 65 Size: 1183 1428 1532 174 1396 Fragments arranged by size: 1532 1428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 2673 96 Size: 2577 3136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 2135 2612 3773 4785 2135 Size: 477 1161 1012 3063 Fragments arranged by size: 3063 1161 1012 477 BstAPI GCAn_nnn'nTGC Cuts at: 794 2248 794 Size: 1454 4259 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 2468 1184 Size: 1284 4429 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2641 2936 2480 Size: 161 295 5257 Fragments arranged by size: 5257 295 161 BstYI r'GATC_y Cuts at: 1972 2193 2898 3043 3410 3795 3812 5264 5350 Size: 221 705 145 367 385 17 1452 86 Cuts at: 5350 5362 5708 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 705 385 367 346 221 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 513 Size: 552 939 63 4159 Fragments arranged by size: 4159 939 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2326 2644 487 Size: 683 56 1100 318 3556 Fragments arranged by size: 3556 1100 683 318 56 BtsI GCAGTG_nn' Cuts at: 2094 3229 4023 4043 2094 Size: 1135 794 20 3764 Fragments arranged by size: 3764 1135 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2505 2540 529 Size: 35 1941 35 3702 Fragments arranged by size: 3702 1941 35 35 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2358 2684 Size: 54 1425 50 275 18 231 297 326 Cuts at: 2684 2922 3160 4049 5678 8 Size: 238 238 889 1629 43 Fragments arranged by size: 1629 1425 889 326 297 275 238 238 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 2684 3160 1537 Size: 275 249 623 476 4090 Fragments arranged by size: 4090 623 476 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 3643 1353 Size: 1556 734 3423 Fragments arranged by size: 3423 1556 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2576 2576 Size: 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2099 2291 2393 2522 2545 2897 Size: 393 451 16 192 102 129 23 352 Cuts at: 2897 3776 4373 5160 5428 1239 Size: 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 451 393 352 268 192 129 102 23 16 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2201 Size: 5713 EcoO109I rG'GnC_Cy Cuts at: 2797 2797 Size: 5713 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 2833 4860 1678 Size: 1155 2027 2531 Fragments arranged by size: 2531 2027 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 4354 4386 4428 Size: 32 135 32 1298 32 1452 32 42 Cuts at: 4428 4460 1373 Size: 32 2626 Fragments arranged by size: 2626 1452 1298 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KasI G'GCGC_C Cuts at: 2829 2829 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4827 5011 1305 Size: 3522 184 2007 Fragments arranged by size: 3522 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 3363 Fragments arranged by size: 3363 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 3671 4030 4189 518 Size: 648 1128 1377 359 159 2042 Fragments arranged by size: 2042 1377 1128 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2331 2356 2587 2689 3129 3806 4954 Size: 471 254 25 231 102 440 677 1148 Cuts at: 4954 5199 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 471 440 254 245 231 102 25 NarI GG'CG_CC Cuts at: 2830 2830 Size: 5713 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4222 Fragments arranged by size: 4222 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 3071 4616 1132 Size: 97 1842 1545 2229 Fragments arranged by size: 2229 1842 1545 97 PasI CC'CwG_GG Cuts at: 1348 2641 1348 Size: 1293 4420 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 2313 2568 2920 1108 Size: 1205 255 352 3901 Fragments arranged by size: 3901 1205 352 255 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 2652 3065 830 Size: 1822 413 3478 Fragments arranged by size: 3478 1822 413 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2077 2356 2077 Size: 279 5434 SacI G_AGCT'C Cuts at: 2578 2578 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2648 3001 3061 4196 4877 5068 826 Size: 219 1603 353 60 1135 681 191 1471 Fragments arranged by size: 1603 1471 1135 681 353 219 191 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2069 2069 Size: 5713 SfoI GGC'GCC Cuts at: 2831 2831 Size: 5713 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 2004 2307 2361 3152 513 Size: 552 939 303 54 791 3074 Fragments arranged by size: 3074 939 791 552 303 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3740 3757 3910 4095 4540 325 Size: 1255 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 2368 2673 Size: 274 80 33 51 1443 177 214 305 Cuts at: 2673 3959 5671 96 Size: 1286 1712 138 Fragments arranged by size: 1712 1443 1286 305 274 214 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 2930 1316 Size: 1614 4099 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2074 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 3842 3842 Size: 5713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI