(Linear) (Six-base) MAPSORT of: KIEE6617.seq Check: 2800 from: 1 to: 1008 KIEE6617.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 1008 Size: 78 930 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 529 561 1008 Size: 529 32 447 Fragments arranged by size: 529 447 32 AlwNI CAG_nnn'CTG Cuts at: 0 243 563 915 1008 Size: 243 320 352 93 Fragments arranged by size: 352 320 243 93 BamHI G'GATC_C Cuts at: 0 188 893 1008 Size: 188 705 115 Fragments arranged by size: 705 188 115 BanI G'GyrC_C Cuts at: 0 824 1008 Size: 824 184 BanII G_rGCy'C Cuts at: 0 573 1008 Size: 573 435 BbeI G_GCGC'C Cuts at: 0 828 1008 Size: 828 180 BglI GCCn_nnn'nGGC Cuts at: 0 64 978 1008 Size: 64 914 30 Fragments arranged by size: 914 64 30 BlpI GC'TnA_GC Cuts at: 0 256 697 1008 Size: 256 441 311 Fragments arranged by size: 441 311 256 BmgBI CAC'GTC Cuts at: 0 759 927 1008 Size: 759 168 81 Fragments arranged by size: 759 168 81 BmrI ACTGGGnnnn_n' Cuts at: 0 573 1008 Size: 573 435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 286 388 517 540 892 1008 Size: 94 192 102 129 23 352 116 Fragments arranged by size: 352 192 129 116 102 94 23 Bpu10I CC'TnA_GC Cuts at: 0 769 1008 Size: 769 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 433 643 1008 Size: 433 210 365 Fragments arranged by size: 433 365 210 BsaAI yAC'GTr Cuts at: 0 42 498 1008 Size: 42 456 510 Fragments arranged by size: 510 456 42 BsaHI Gr'CG_yC Cuts at: 0 825 1008 Size: 825 183 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 865 895 1008 Size: 865 30 113 Fragments arranged by size: 865 113 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 625 1008 Size: 625 383 BseYI C'CCAG_C Cuts at: 0 68 233 575 1008 Size: 68 165 342 433 Fragments arranged by size: 433 342 165 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 673 1008 Size: 535 138 335 Fragments arranged by size: 535 335 138 BsiEI CG_ry'CG Cuts at: 0 59 682 876 1008 Size: 59 623 194 132 Fragments arranged by size: 623 194 132 59 BsiHKAI G_wGCw'C Cuts at: 0 573 1008 Size: 573 435 Bsp1286I G_dGCh'C Cuts at: 0 573 1008 Size: 573 435 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 100 110 173 860 1008 Size: 100 10 63 687 148 Fragments arranged by size: 687 148 100 63 10 BsrBI CCG'CTC Cuts at: 0 810 957 1008 Size: 810 147 51 Fragments arranged by size: 810 147 51 BsrDI GCAATG_nn' Cuts at: 0 671 1008 Size: 671 337 BsrGI T'GTAC_A Cuts at: 0 668 1008 Size: 668 340 BssSI C'ACGA_G Cuts at: 0 130 607 1008 Size: 130 477 401 Fragments arranged by size: 477 401 130 BstAPI GCAn_nnn'nTGC Cuts at: 0 243 1008 Size: 243 765 BstEII G'GTnAC_C Cuts at: 0 463 1008 Size: 463 545 BstXI CCAn_nnnn'nTGG Cuts at: 0 475 636 931 1008 Size: 475 161 295 77 Fragments arranged by size: 475 295 161 77 BstYI r'GATC_y Cuts at: 0 188 893 1008 Size: 188 705 115 Fragments arranged by size: 705 188 115 BtgI C'CryG_G Cuts at: 0 62 1008 Size: 62 946 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 321 639 1008 Size: 321 318 369 Fragments arranged by size: 369 321 318 BtsI GCAGTG_nn' Cuts at: 0 89 1008 Size: 89 919 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 500 535 1008 Size: 500 35 473 Fragments arranged by size: 500 473 35 EaeI y'GGCC_r Cuts at: 0 56 353 679 917 1008 Size: 56 297 326 238 91 Fragments arranged by size: 326 297 238 91 56 EagI C'GGCC_G Cuts at: 0 56 679 1008 Size: 56 623 329 Fragments arranged by size: 623 329 56 EarI CTCTTCn'nnn_ Cuts at: 0 904 1008 Size: 904 104 EcoICRI GAG'CTC Cuts at: 0 571 1008 Size: 571 437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 94 286 388 517 540 892 1008 Size: 78 16 192 102 129 23 352 116 Fragments arranged by size: 352 192 129 116 102 78 23 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 196 1008 Size: 196 812 EcoO109I rG'GnC_Cy Cuts at: 0 792 1008 Size: 792 216 HaeII r_GCGC'y Cuts at: 0 828 1008 Size: 828 180 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 865 897 1008 Size: 865 32 111 Fragments arranged by size: 865 111 32 KasI G'GCGC_C Cuts at: 0 824 1008 Size: 824 184 MscI TGG'CCA Cuts at: 0 355 1008 Size: 355 653 MslI CAynn'nnrTG Cuts at: 0 289 1008 Size: 289 719 MspA1I CmG'CkG Cuts at: 0 72 326 351 582 684 1008 Size: 72 254 25 231 102 324 Fragments arranged by size: 324 254 231 102 72 25 NarI GG'CG_CC Cuts at: 0 825 1008 Size: 825 183 PasI CC'CwG_GG Cuts at: 0 636 1008 Size: 636 372 PflMI CCAn_nnn'nTGG Cuts at: 0 308 563 915 1008 Size: 308 255 352 93 Fragments arranged by size: 352 308 255 93 PstI C_TGCA'G Cuts at: 0 647 1008 Size: 647 361 PvuII CAG'CTG Cuts at: 0 72 351 1008 Size: 72 279 657 Fragments arranged by size: 657 279 72 SacI G_AGCT'C Cuts at: 0 573 1008 Size: 573 435 SfcI C'TryA_G Cuts at: 0 643 996 1008 Size: 643 353 12 Fragments arranged by size: 643 353 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 64 1008 Size: 64 944 SfoI GGC'GCC Cuts at: 0 826 1008 Size: 826 182 StyI C'CwwG_G Cuts at: 0 302 356 1008 Size: 302 54 652 Fragments arranged by size: 652 302 54 TatI w'GTAC_w Cuts at: 0 149 363 668 1008 Size: 149 214 305 340 Fragments arranged by size: 340 305 214 149 Tth111I GACn'n_nGTC Cuts at: 0 925 1008 Size: 925 83 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 69 1008 Size: 69 939 Enzymes that do cut: AcuI AloI AlwNI BamHI BanI BanII BbeI BglI BlpI BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NarI PasI PflMI PstI PvuII SacI SfcI SfiI SfoI StyI TatI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XhoI XmaI XmnI ZraI