################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BclI BfaI BfrI BglI BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MluI MluNI MroI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 640 CGCG CG^CG AcyI 703 GGCGTC G[GA]^CG[CT]C AlwNI 325 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 675 ATTAAT AT^TAAT AsnI 675 ATTAAT AT^TAAT AspHI 247 GAGCAC G[AT]GC[AT]^C AvaII 149 GGACC G^G[AT]CC BanII 503 GGGCTC G[GA]GC[CT]^C BbrPI 87 CACGTG CAC^GTG BciVI 215 GTATCC GTATCC(6/5) BglII 509 AGATCT A^GATCT BsaAI 87 CACGTG [CT]AC^GT[GA] BsaHI 703 GGCGTC G[GA]^CG[CT]C BseRI 307 CTCCTC GAGGAG(10/8) BsgI 348 GTGCAG GTGCAG(16/14) BsiHKAI 247 GAGCAC G[AT]GC[AT]^C BsmI 870 GAATGC GAATGC(1/-1) BspHI 416 TCATGA T^CATGA BsrDI 92 GCAATG GCAATG(2/0) BstUI 640 CGCG CG^CG BstXI 194 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 509 AGATCT [GA]^GATC[CT] Cfr13I 149 GGACC G^G[ACGT]CC EaeI 342 CGGCCA [CT]^GGCC[GA] EcoRI 631 GAATTC G^AATTC FauI 298 GCGGG CCCGC(4/6) HgaI 704 GCGTC GACGC(5/10) Hin1I 703 GGCGTC G[GA]^CG[CT]C MaeII 88 ACGT A^CGT MfeI 210 CAATTG C^AATTG MflI 509 AGATCT [GA]^GATC[CT] MseI 676 TTAA T^TAA MspA1I 199 CAGCTG C[AC]G^C[GT]G MunI 210 CAATTG C^AATTG MvnI 640 CGCG CG^CG MwoI 289 GCAGGGACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 173 GGCTCC GG[ACGT]^[ACGT]CC PmaCI 87 CACGTG CAC^GTG PmlI 87 CACGTG CAC^GTG PshBI 675 ATTAAT AT^TAAT PstI 255 CTGCAG CTGCA^G PvuII 199 CAGCTG CAG^CTG RcaI 416 TCATGA T^CATGA Sau96I 149 GGACC G^G[ACGT]CC ScaI 143 AGTACT AGT^ACT SexAI 158 ACCAGGT A^CC[AT]GGT SmaI 281 CCCGGG CCC^GGG TaqI 233 TCGA T^CGA Tru9I 676 TTAA T^TAA TthHB8I 233 TCGA T^CGA XhoII 509 AGATCT [GA]^GATC[CT] XmaCI 281 CCCGGG C^CCGGG XmaI 281 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 144 GTAC GT^AC 156 GTAC BlpI 736 GCTCAGC GC^T[ACGT]AGC 786 GCTCAGC BmyI 247 GAGCAC G[AGT]GC[ACT]^C 503 GGGCTC BpmI 175 CTCCAG CTGGAG(16/14) 826 CTGGAG Bpu1102I 736 GCTCAGC GC^T[ACGT]AGC 786 GCTCAGC BsmFI 292 GGGAC GGGAC(10/14) 616 GGGAC Bsp1286I 247 GAGCAC G[AGT]GC[ACT]^C 503 GGGCTC Cac8I 63 GCCAGC GC[ACGT]^[ACGT]GC 245 GCGAGC CelII 736 GCTCAGC GC^T[ACGT]AGC 786 GCTCAGC DpnII 510 GATC ^GATC 695 GATC HaeIII 118 GGCC GG^CC 343 GGCC MboI 510 GATC ^GATC 695 GATC NdeII 510 GATC ^GATC 695 GATC PleI 618 GACTC GAGTC(4/5) 547 GAGTC RsaI 144 GTAC GT^AC 156 GTAC Sau3AI 510 GATC ^GATC 695 GATC SfaNI 205 GATGC GCATC(5/9) 327 GCATC SfcI 255 CTGCAG C^T[GA][CT]AG 312 CTACAG TfiI 3 GATTC G^A[AT]TC 27 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcsI AluI ApoI AvaI BbsI BbvI BcnI BpuAI BsaJI BsiYI BslI BsoBI BsrI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MspI MvaI NciI NlaIII ScrFI TseI Tsp45I Tsp509I TspRI