(Circular) (Six-base) MAPSORT of: pFN21AB7609.seq Check: 6571 from: 1 to: 5605 pFN21AB7609 5605 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 2904 3732 4105 1563 Size: 1341 828 373 3063 Fragments arranged by size: 3063 1341 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2698 3668 5052 5320 1632 Size: 725 341 970 1384 268 1917 Fragments arranged by size: 1917 1384 970 725 341 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2288 1184 Size: 1104 4501 AlwNI CAG_nnn'CTG Cuts at: 2335 3348 4920 2335 Size: 1013 1572 3020 Fragments arranged by size: 3020 1572 1013 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2136 2636 2826 2864 2914 3140 3292 5401 Size: 643 500 190 38 50 226 152 2109 Cuts at: 5401 1493 Size: 1697 Fragments arranged by size: 2109 1697 643 500 226 190 152 50 38 AseI AT'TA_AT Cuts at: 160 1032 2681 4159 160 Size: 872 1649 1478 1606 Fragments arranged by size: 1649 1606 1478 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2286 2614 2880 2930 1870 Size: 73 343 328 266 50 4545 Fragments arranged by size: 4545 343 328 266 73 50 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2512 2924 1391 Size: 1121 412 4072 Fragments arranged by size: 4072 1121 412 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 2704 2857 928 Size: 971 801 4 153 3676 Fragments arranged by size: 3676 971 801 153 4 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 3497 4707 2231 Size: 1266 1210 3129 Fragments arranged by size: 3129 1266 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2514 5600 2514 Size: 3086 2519 BlpI GC'TnA_GC Cuts at: 2742 2792 3022 2742 Size: 50 230 5325 Fragments arranged by size: 5325 230 50 Bme1580I G_kGCm'C Cuts at: 1790 3666 4822 1790 Size: 1876 1156 2573 Fragments arranged by size: 2573 1876 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2640 2640 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2852 4265 1239 Size: 926 687 1413 2579 Fragments arranged by size: 2579 1413 926 687 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4268 882 Size: 617 2769 2219 Fragments arranged by size: 2769 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2094 3396 493 Size: 711 890 1302 2702 Fragments arranged by size: 2702 1302 890 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2709 3412 3794 4429 Size: 53 83 186 936 1176 703 382 635 Cuts at: 4429 275 Size: 1451 Fragments arranged by size: 1451 1176 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 2072 2102 1178 Size: 30 165 30 607 30 32 30 4681 Fragments arranged by size: 4681 607 165 32 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 1186 Size: 1117 4488 BseYI C'CCAG_C Cuts at: 4808 5304 4808 Size: 496 5109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 1163 Size: 573 638 4394 Fragments arranged by size: 4394 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 3666 3751 4822 2256 Size: 1410 85 1071 3039 Fragments arranged by size: 3039 1410 1071 85 BsmI GAATG_Cn' Cuts at: 2881 3116 3209 2881 Size: 235 93 5277 Fragments arranged by size: 5277 235 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2512 2924 3666 3751 4822 1391 Size: 399 466 256 412 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 412 399 256 85 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 2421 3494 5224 2421 Size: 1073 1730 2802 Fragments arranged by size: 2802 1730 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2104 4100 4274 65 Size: 1183 856 1996 174 1396 Fragments arranged by size: 1996 1396 1183 856 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2206 2011 Size: 195 5410 BstYI r'GATC_y Cuts at: 1972 2514 2935 3302 3687 3704 5156 5242 5254 Size: 542 421 367 385 17 1452 86 12 Cuts at: 5254 5600 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 542 421 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5053 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2742 3121 3915 3935 2742 Size: 379 794 20 4412 Fragments arranged by size: 4412 794 379 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 3052 3941 Size: 54 1425 50 275 18 517 705 889 Cuts at: 3941 5570 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 705 517 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3535 1353 Size: 2182 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2357 2698 2852 3668 4265 5052 Size: 393 533 192 341 154 816 597 787 Cuts at: 5052 5320 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 597 533 393 341 268 192 154 EcoRI G'AATT_C Cuts at: 2636 2914 3292 2636 Size: 278 378 4949 Fragments arranged by size: 4949 378 278 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 4246 4278 4320 Size: 32 135 32 500 32 2142 32 42 Cuts at: 4320 4352 1373 Size: 32 2626 Fragments arranged by size: 2626 2142 500 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 2215 3204 2215 Size: 989 4616 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 4719 4903 1305 Size: 1161 2253 184 2007 Fragments arranged by size: 2253 2007 1161 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3783 Fragments arranged by size: 3783 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3922 4081 518 Size: 648 2397 359 159 2042 Fragments arranged by size: 2397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 3021 3698 4846 5091 1606 Size: 600 815 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 677 600 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5053 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PmlI CAC'GTG Cuts at: 2094 2094 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2264 2957 830 Size: 1434 693 3478 Fragments arranged by size: 3478 1434 693 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2206 2206 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 2150 3853 5565 2150 Size: 1703 1712 2190 Fragments arranged by size: 2190 1712 1703 SexAI A'CCwGG_T Cuts at: 2163 2163 Size: 5605 SfcI C'TryA_G Cuts at: 826 1045 2260 2317 2953 4088 4769 4960 826 Size: 219 1215 57 636 1135 681 191 1471 Fragments arranged by size: 1471 1215 1135 681 636 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2288 2932 1872 Size: 416 644 4545 Fragments arranged by size: 4545 644 416 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 3044 513 Size: 552 1979 3074 Fragments arranged by size: 3074 1979 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2712 3632 3649 3802 3987 4432 325 Size: 1255 1132 920 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1132 920 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 3851 5563 Size: 274 80 33 51 1443 171 1703 1712 Cuts at: 5563 96 Size: 138 Fragments arranged by size: 1712 1703 1443 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2286 2930 1870 Size: 416 644 4545 Fragments arranged by size: 4545 644 416 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI