Restriction map for
pFN21AE5556
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccIII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BfaI BfrI BglI BglII BlnI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NgoMIV
NheI NotI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 961 GTCTAC GT^[AC][GT]AC
AccII 280 CGCG CG^CG
AcyI 940 GACGCC G[GA]^CG[CT]C
AvaI 599 CTCGAG C^[CT]CG[GA]G
BciVI 202 GTATCC GTATCC(6/5)
BclI 975 TGATCA T^GATCA
BcnI 9 CCCGG CC^[GC]GG
BlpI 46 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
BsaHI 940 GACGCC G[GA]^CG[CT]C
BsiYI 330 CCACGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 330 CCACGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 296 CGTCTC CGTCTC(1/5)
BsmFI 7 GTCCC GGGAC(10/14)
BsoBI 599 CTCGAG C^[CT]CG[GA]G
BspHI 711 TCATGA T^CATGA
BstUI 280 CGCG CG^CG
BstYI 639 AGATCC [GA]^GATC[CT]
Cac8I 701 GCGAGC GC[ACGT]^[ACGT]GC
CelII 46 GCTGAGC GC^T[ACGT]AGC
Cfr13I 404 GGGCC G^G[ACGT]CC
DraI 675 TTTAAA TTT^AAA
DsaI 330 CCACGG C^C[GA][CT]GG
EcoT22I 647 ATGCAT ATGCA^T
FbaI 975 TGATCA T^GATCA
HapII 10 CCGG C^CGG
Hin1I 940 GACGCC G[GA]^CG[CT]C
HindIII 239 AAGCTT A^AGCTT
HpaII 10 CCGG C^CGG
MflI 639 AGATCC [GA]^GATC[CT]
MslI 726 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 10 CCGG C^CGG
MvnI 280 CGCG CG^CG
NciI 9 CCCGG CC^[GC]GG
NdeI 752 CATATG CA^TATG
NruI 279 TCGCGA TCG^CGA
NsiI 647 ATGCAT ATGCA^T
PaeR7I 599 CTCGAG C^TCGAG
PflMI 330 CCACGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1107 GAGTC GAGTC(4/5)
PstI 307 CTGCAG CTGCA^G
RcaI 711 TCATGA T^CATGA
Sau96I 404 GGGCC G^G[ACGT]CC
SfcI 307 CTGCAG C^T[GA][CT]AG
SmlI 599 CTCGAG C^T[CT][GA]AG
Van91I 330 CCACGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 599 CTCGAG C^TCGAG
XhoII 639 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 274 AAATTT [GA]^AATT[CT]
872 AAATTT
AfaI 414 GTAC GT^AC
625 GTAC
ApoI 274 AAATTT [GA]^AATT[CT]
872 AAATTT
BsaJI 330 CCACGG C^C[ACGT][ACGT]GG
593 CCCAGG
EarI 91 GAAGAG CTCTTC(1/4)
528 GAAGAG
HgaI 940 GACGC GACGC(5/10)
5 GCGTC
HphI 286 GGTGA GGTGA(8/7)
467 TCACC
Ksp632I 91 GAAGAG CTCTTC(1/4)
528 GAAGAG
MaeIII 572 GTTAC ^GT[ACGT]AC
804 GTTAC
MwoI 589 GCTTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
739 GCTAAATTGGC
NlaIV 222 GGAACC GG[ACGT]^[ACGT]CC
403 GGGGCC
RsaI 414 GTAC GT^AC
625 GTAC
TaqI 20 TCGA T^CGA
600 TCGA
TfiI 462 GAATC G^A[AT]TC
790 GATTC
TthHB8I 20 TCGA T^CGA
600 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AlwI BbvI BsmAI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
SfaNI Tru9I TseI Tsp509I TspRI