Restriction map for
pFN21AE5556
(Circular) (Six-base) MAPSORT of: pFN21AE5556.seq Check: 2496 from: 1 to: 5827
pFN21AE5556 5827 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2967 3170 1937
Size: 1030 203 4594
Fragments arranged by size:
4594 1030 203
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2891 3890 5274 5542 1632
Size: 551 708 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 708 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 3570 5142 3570
Size: 1572 4255
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2279 2877 3136 3362 3514 5623 1493
Size: 786 598 259 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 598 259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2604 3152 1870
Size: 73 661 548 4545
Fragments arranged by size:
4545 661 548 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 4603 618
Size: 325 217 627 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3146 1391
Size: 1755 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2218 3719 4929 2218
Size: 1501 1210 3116
Fragments arranged by size:
3116 1501 1210
BclI T'GATC_A
Cuts at: 1548 2980 1548
Size: 1432 4395
BfrBI ATG'CAT
Cuts at: 2654 2654
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 2052 3244 2052
Size: 1192 4635
Bme1580I G_kGCm'C
Cuts at: 1790 3888 5044 1790
Size: 2098 1156 2573
Fragments arranged by size:
2573 2098 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4516 442
Size: 416 53 83 399 24 2141 958 1753
Fragments arranged by size:
2141 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4487 1239
Size: 3248 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2946 3634 4016 4651
Size: 53 83 186 936 1413 688 382 635
Cuts at: 4651 275
Size: 1451
Fragments arranged by size:
1451 1413 936 688 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3888 3973 5044 3888
Size: 85 1071 4671
Fragments arranged by size:
4671 1071 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
BsmBI CGTCTCn'nnnn_
Cuts at: 2307 2307
Size: 5827
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3146 3888 3973 5044 1391
Size: 399 1356 742 85 1071 2174
Fragments arranged by size:
2174 1356 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 2716 3716 5446 2716
Size: 1000 1730 3097
Fragments arranged by size:
3097 1730 1000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 844
Size: 2338 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3714 1394
Size: 429 1891 3507
Fragments arranged by size:
3507 1891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2644 3157 3524 3909 3926 5378 5464 5476
Size: 672 513 367 385 17 1452 86 12
Cuts at: 5476 5822 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 672 513 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2335 513
Size: 552 1270 4005
Fragments arranged by size:
4005 1270 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 3047 3343 4137 4157 3047
Size: 296 794 20 4717
Fragments arranged by size:
4717 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 2682 3131 2682
Size: 449 5378
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3274 4163 5792
Size: 54 1425 50 275 18 1444 889 1629
Cuts at: 5792 8
Size: 43
Fragments arranged by size:
1629 1444 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2528 3757 1353
Size: 738 437 1229 3423
Fragments arranged by size:
3423 1229 738 437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2891 3890 4487 5274 5542 1239
Size: 393 551 708 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 708 597 551 393 268
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2132 2726 2132
Size: 594 5233
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2244 748
Size: 1496 4331
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2735 3785 4144 4303 518
Size: 648 1569 1050 359 159 2042
Fragments arranged by size:
2042 1569 1050 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3243 3920 5068 5313 1606
Size: 1637 677 1148 245 2120
Fragments arranged by size:
2120 1637 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5275
NdeI CA'TA_TG
Cuts at: 387 2758 387
Size: 2371 3456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NruI TCG'CGA
Cuts at: 2286 2286
Size: 5827
NsiI A_TGCA'T
Cuts at: 2656 2656
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 1108
Size: 1233 4594
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspXI vC'TCGA_Gb
Cuts at: 2604 2604
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2316 3179 830
Size: 1486 863 3478
Fragments arranged by size:
3478 1486 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2312 3175 4310 4991 5182 826
Size: 219 1267 863 1135 681 191 1471
Fragments arranged by size:
1471 1267 1135 863 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2604 3931 4832 5094 820
Size: 197 926 661 1327 901 262 1553
Fragments arranged by size:
1553 1327 926 901 661 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 3266 513
Size: 552 2201 3074
Fragments arranged by size:
3074 2201 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 4073 5785
Size: 274 80 33 51 1443 441 1655 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1712 1655 1443 441 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 2604 1943
Size: 661 5166
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV PacI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI