################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeIII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 672 GCGG CCGC(-3/-1) AvaI 309 CTCGGG C^[CT]CG[GA]G BbsI 573 GTCTTC GAAGAC(2/6) BpuAI 573 GTCTTC GAAGAC(2/6) BsaBI 49 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 903 GGTCTC GGTCTC(1/5) BseRI 758 GAGGAG GAGGAG(10/8) BsgI 727 CTGCAC GTGCAG(16/14) BsmI 809 GAATGC GAATGC(1/-1) BsoBI 309 CTCGGG C^[CT]CG[GA]G BspLU11I 738 ACATGT A^CATGT BsrBI 670 GAGCGG CCGCTC(-3/-3) EaeI 456 TGGCCG [CT]^GGCC[GA] EarI 712 CTCTTC CTCTTC(1/4) EcoT14I 681 CCAAGG C^C[AT][AT]GG EcoT22I 52 ATGCAT ATGCA^T HapII 459 CCGG C^CGG HpaI 129 GTTAAC GTT^AAC HpaII 459 CCGG C^CGG HphI 553 TCACC GGTGA(8/7) Ksp632I 712 CTCTTC CTCTTC(1/4) MaeII 382 ACGT A^CGT MamI 49 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 717 CATTAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 459 CCGG C^CGG NheI 72 GCTAGC G^CTAGC NsiI 52 ATGCAT ATGCA^T NspI 738 ACATGT [GA]CATG^[CT] PstI 814 CTGCAG CTGCA^G SfcI 814 CTGCAG C^T[GA][CT]AG StyI 681 CCAAGG C^C[AT][AT]GG XcmI 470 CCAGGTATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 25 GTAC GT^AC 590 GTAC AflIII 382 ACGTGT A^C[GA][CT]GT 738 ACATGT Alw44I 3 GTGCAC G^TGCAC 302 GTGCAC ApaLI 3 GTGCAC G^TGCAC 302 GTGCAC AspHI 3 GTGCAC G[AT]GC[AT]^C 302 GTGCAC AvaII 408 GGTCC G^G[AT]CC 747 GGTCC BanI 190 GGTGCC G^G[CT][GA]CC 356 GGTGCC BanII 15 GAGCCC G[GA]GC[CT]^C 312 GGGCTC BfaI 73 CTAG C^TAG 448 CTAG BglII 114 AGATCT A^GATCT 818 AGATCT BsaJI 308 CCTCGG C^C[ACGT][ACGT]GG 681 CCAAGG BsiHKAI 3 GTGCAC G[AT]GC[AT]^C 302 GTGCAC Cac8I 72 GCTAGC GC[ACGT]^[ACGT]GC 76 GCCAGC DdeI 13 CTGAG C^T[ACGT]AG 923 CTAAG HincII 129 GTTAAC GT[CT]^[GA]AC 203 GTCAAC HindII 129 GTTAAC GT[CT]^[GA]AC 203 GTCAAC MaeI 73 CTAG C^TAG 448 CTAG MseI 130 TTAA T^TAA 935 TTAA RsaI 25 GTAC GT^AC 590 GTAC Tru9I 130 TTAA T^TAA 935 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BmyI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MflI MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI XhoII