(Circular) (Six-base) MAPSORT of: pFN21AE1801.seq Check: 9238 from: 1 to: 5644 pFN21AE1801 5644 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2655 3707 5091 5359 1632 Size: 1023 1052 1384 268 1917 Fragments arranged by size: 1917 1384 1052 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2387 2743 4543 5520 1203 Size: 1184 356 1800 977 1327 Fragments arranged by size: 1800 1327 1184 977 356 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 2008 2307 3701 4857 2008 Size: 299 1394 1156 2795 Fragments arranged by size: 2795 1394 1156 299 ApoI r'AATT_y Cuts at: 1493 2234 2347 2628 2953 3179 3331 5440 1493 Size: 741 113 281 325 226 152 2109 1697 Fragments arranged by size: 2109 1697 741 325 281 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2314 2969 1870 Size: 73 371 655 4545 Fragments arranged by size: 4545 655 371 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 2361 2965 4420 618 Size: 325 217 627 408 166 604 1455 1842 Fragments arranged by size: 1842 1455 627 604 408 325 217 166 BanII G_rGCy'C Cuts at: 1391 2024 2321 2963 1391 Size: 633 297 642 4072 Fragments arranged by size: 4072 642 633 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2571 928 Size: 971 672 4001 Fragments arranged by size: 4001 971 672 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BfrBI ATG'CAT Cuts at: 2059 2059 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2119 2823 5639 2119 Size: 704 2816 2124 Fragments arranged by size: 2816 2124 704 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2012 2311 3705 4861 1790 Size: 222 299 1394 1156 2573 Fragments arranged by size: 2573 1394 1156 299 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2081 1056 Size: 1025 4619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4304 1239 Size: 3065 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2914 4307 882 Size: 617 1415 1393 2219 Fragments arranged by size: 2219 1415 1393 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 2058 5638 2058 Size: 3580 2064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2778 1186 Size: 1592 4052 BseYI C'CCAG_C Cuts at: 2769 4847 5343 2769 Size: 2078 496 3070 Fragments arranged by size: 3070 2078 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2717 1163 Size: 573 981 4090 Fragments arranged by size: 4090 981 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2311 3705 3790 4861 2012 Size: 299 1394 85 1071 2795 Fragments arranged by size: 2795 1394 1071 299 85 BsmI GAATG_Cn' Cuts at: 2820 3155 3248 2820 Size: 335 93 5216 Fragments arranged by size: 5216 335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2024 2311 2321 2963 3705 3790 Size: 399 222 12 287 10 642 742 85 Cuts at: 3790 4861 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 642 399 287 222 85 12 10 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2677 3531 1394 Size: 429 854 854 3507 Fragments arranged by size: 3507 854 854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2119 2289 2638 2712 2823 2974 3341 3726 Size: 147 170 349 74 111 151 367 385 Cuts at: 3726 3743 5195 5281 5293 5639 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 349 346 170 151 147 111 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5092 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2010 2041 2625 3160 3954 3974 2010 Size: 31 584 535 794 20 3680 Fragments arranged by size: 3680 794 584 535 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2461 3091 3980 Size: 54 1425 50 275 18 631 630 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 631 630 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 3574 1353 Size: 1370 851 3423 Fragments arranged by size: 3423 1370 851 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2655 3707 4304 5091 5359 1239 Size: 393 1023 1052 597 787 268 1524 Fragments arranged by size: 1524 1052 1023 787 597 393 268 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2555 2734 2555 Size: 179 5465 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2697 2729 4285 4317 4359 Size: 32 135 32 1125 32 1556 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1556 1125 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2136 2210 2988 3234 669 Size: 988 281 198 74 778 246 3079 Fragments arranged by size: 3079 988 778 281 246 198 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 2136 3234 2136 Size: 1098 4546 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2894 4758 4942 1305 Size: 861 728 1864 184 2007 Fragments arranged by size: 2007 1864 861 728 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2726 3602 3961 4120 518 Size: 648 1560 876 359 159 2042 Fragments arranged by size: 2042 1560 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5092 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 2077 1052 Size: 1025 4619 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NsiI A_TGCA'T Cuts at: 2061 2061 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2747 3002 4547 1132 Size: 97 1518 255 1545 2229 Fragments arranged by size: 2229 1545 1518 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2743 4543 2743 Size: 1800 3844 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 2922 3214 2922 Size: 292 5352 PstI C_TGCA'G Cuts at: 830 2823 2996 830 Size: 1993 173 3478 Fragments arranged by size: 3478 1993 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2819 2992 4127 4808 4999 826 Size: 219 1774 173 1135 681 191 1471 Fragments arranged by size: 1774 1471 1135 681 219 191 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2686 3083 513 Size: 552 1621 397 3074 Fragments arranged by size: 3074 1621 552 397 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3671 3688 3841 4026 4471 325 Size: 1255 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2594 3890 5602 Size: 274 80 33 51 1443 617 1296 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1443 1296 617 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 5604 5636 2372 Size: 32 3200 32 2380 Fragments arranged by size: 3200 2380 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2482 2482 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI