################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## Acc65I AccII AccIII AclI AflIII AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BlnI BlpI Bpu1102I BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AatII 1278 GACGTC GACGT^C AccI 1072 GTCTAC GT^[AC][GT]AC AcyI 1278 GACGTC G[GA]^CG[CT]C AflII 292 CTTAAG C^TTAAG AgeI 420 ACCGGT A^CCGGT Alw44I 1341 GTGCAC G^TGCAC ApaI 1413 GGGCCC GGGCC^C ApaLI 1341 GTGCAC G^TGCAC Asp700I 940 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 527 TGGCCA TGG^CCA BanII 1413 GGGCCC G[GA]GC[CT]^C BbrPI 536 CACGTG CAC^GTG BbsI 1227 GAAGAC GAAGAC(2/6) BclI 852 TGATCA T^GATCA BcnI 33 CCGGG CC^[GC]GG BfaI 617 CTAG C^TAG BfrI 292 CTTAAG C^TTAAG BglI 520 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1227 GAAGAC GAAGAC(2/6) BsaAI 536 CACGTG [CT]AC^GT[GA] BsaBI 112 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1278 GACGTC G[GA]^CG[CT]C BsaI 1585 GGTCTC GGTCTC(1/5) BsaWI 420 ACCGGT [AT]^CCGG[AT] BseRI 52 GAGGAG GAGGAG(10/8) BsiEI 30 CGGCCG CG[GA][CT]^CG BsmAI 1586 GTCTC GTCTC(1/5) BsmFI 83 GGGAC GGGAC(10/14) BstAPI 505 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 334 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 126 CCACGG C^C[GA][CT]GG EagI 30 CGGCCG C^GGCCG EarI 352 GAAGAG CTCTTC(1/4) EclXI 30 CGGCCG C^GGCCG Eco52I 30 CGGCCG C^GGCCG EcoNI 633 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 340 CCTTGG C^C[AT][AT]GG FauI 80 GCGGG CCCGC(4/6) FbaI 852 TGATCA T^GATCA HgaI 13 GCGTC GACGC(5/10) Hin1I 1278 GACGTC G[GA]^CG[CT]C Ksp632I 352 GAAGAG CTCTTC(1/4) MaeI 617 CTAG C^TAG MamI 112 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1349 CAATTG C^AATTG MluNI 527 TGGCCA TGG^CCA MscI 527 TGGCCA TGG^CCA MspA1I 78 CAGCGG C[AC]G^C[GT]G MunI 1349 CAATTG C^AATTG NciI 33 CCGGG CC^[GC]GG NdeI 490 CATATG CA^TATG NlaIV 1413 GGGCCC GG[ACGT]^[ACGT]CC NspI 1125 ACATGC [GA]CATG^[CT] PflMI 873 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 420 ACCGGT A^CCGGT PmaCI 536 CACGTG CAC^GTG PmlI 536 CACGTG CAC^GTG PpuMI 56 AGGACCT [GA]G^G[AT]CC[CT] SmlI 292 CTTAAG C^T[CT][GA]AG SpeI 616 ACTAGT A^CTAGT SspI 220 AATATT AAT^ATT StyI 340 CCTTGG C^C[AT][AT]GG Van91I 873 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 940 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 312 GTAC GT^AC 430 GTAC AlwNI 597 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1199 CAGAAGCTG AvaII 57 GGACC G^G[AT]CC 630 GGACC BbvI 135 GCAGC GCAGC(8/12) 817 GCTGC BciVI 262 GGATAC GTATCC(6/5) 683 GGATAC BglII 117 AGATCT A^GATCT 408 AGATCT BpmI 634 CTCCAG CTGGAG(16/14) 1588 CTCCAG BsrDI 624 CATTGC GCAATG(2/0) 877 GCAATG BsrFI 25 ACCGGC [GA]^CCGG[CT] 420 ACCGGT Cac8I 131 GCAAGC GC[ACGT]^[ACGT]GC 1354 GCTGGC CfoI 20 GCGC GCG^C 38 GCGC Cfr10I 25 ACCGGC [GA]^CCGG[CT] 420 ACCGGT DraII 56 AGGACCT [GA]G^G[ACGT]CC[CT] 1413 GGGCCCT EaeI 30 CGGCCG [CT]^GGCC[GA] 527 TGGCCA EcoO109I 56 AGGACCT [GA]G^G[ACGT]CC[CT] 1413 GGGCCCT EcoRI 318 GAATTC G^AATTC 800 GAATTC HaeII 19 GGCGCT [GA]GCGC^[CT] 37 GGCGCT HhaI 20 GCGC GCG^C 38 GCGC HinP1I 20 GCGC G^CGC 38 GCGC HphI 1486 GGTGA GGTGA(8/7) 1544 TCACC RsaI 312 GTAC GT^AC 430 GTAC SfcI 302 CTACAG C^T[GA][CT]AG 956 CTGTAG TfiI 297 GAATC G^A[AT]TC 1024 GATTC TseI 135 GCAGC G^C[AT]GC 817 GCTGC Tsp45I 1507 GTCAC ^GT[GC]AC 1549 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp509I TspRI XhoII