(Circular) (Six-base) MAPSORT of: pFN21AE1968.seq Check: 8342 from: 1 to: 6298 pFN21AE1968 6298 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3479 3479 Size: 6298 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3287 4108 278 Size: 53 83 186 936 1751 821 2468 Fragments arranged by size: 2468 1751 936 821 186 83 53 AccI GT'mk_AC Cuts at: 1937 3078 3641 1937 Size: 1141 563 4594 Fragments arranged by size: 4594 1141 563 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2205 2541 4361 5745 6013 1632 Size: 573 336 1820 1384 268 1917 Fragments arranged by size: 1917 1820 1384 573 336 268 AflII C'TTAA_G Cuts at: 820 1017 2297 820 Size: 197 1280 4821 Fragments arranged by size: 4821 1280 197 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 2425 5129 2425 Size: 2704 3594 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6298 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AlwNI CAG_nnn'CTG Cuts at: 2607 3209 4041 5613 2607 Size: 602 832 1572 3292 Fragments arranged by size: 3292 1572 832 602 ApaI G_GGCC'C Cuts at: 3422 3422 Size: 6298 ApaLI G'TGCA_C Cuts at: 3346 4355 5511 3346 Size: 1009 1156 4133 Fragments arranged by size: 4133 1156 1009 ApoI r'AATT_y Cuts at: 1493 2258 2284 2323 2805 3302 3607 3833 3985 Size: 765 26 39 482 497 305 226 152 Cuts at: 3985 6094 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 765 497 482 305 226 152 39 26 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 3623 1870 Size: 73 1680 4545 Fragments arranged by size: 4545 1680 73 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3619 5074 618 Size: 325 217 627 1832 1455 1842 Fragments arranged by size: 1842 1832 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3422 3617 1391 Size: 2031 195 4072 Fragments arranged by size: 4072 2031 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3239 928 Size: 971 1340 3987 Fragments arranged by size: 3987 1340 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2261 2682 4190 5400 2261 Size: 421 1508 1210 3159 Fragments arranged by size: 3159 1508 1210 421 BclI T'GATC_A Cuts at: 1548 2857 1548 Size: 1309 4989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2531 4909 136 Size: 107 122 71 631 605 859 2378 1525 Fragments arranged by size: 2378 1525 859 631 605 122 107 71 BglII A'GATC_T Cuts at: 2122 2413 6293 2122 Size: 291 3880 2127 Fragments arranged by size: 3880 2127 291 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 2512 3350 3422 4359 5515 1790 Size: 722 838 72 937 1156 2573 Fragments arranged by size: 2573 1156 937 838 722 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2333 4029 4987 Size: 416 53 83 399 24 916 1696 958 Cuts at: 4987 442 Size: 1753 Fragments arranged by size: 1753 1696 958 916 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 3578 4958 1239 Size: 1385 954 1380 2579 Fragments arranged by size: 2579 1385 1380 954 Bpu10I CC'TnA_GC Cuts at: 2574 2574 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4423 5288 5586 4423 Size: 865 298 5135 Fragments arranged by size: 5135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3596 4961 882 Size: 617 2097 1365 2219 Fragments arranged by size: 2219 2097 1365 617 BsaAI yAC'GTr Cuts at: 493 1204 2543 4089 493 Size: 711 1339 1546 2702 Fragments arranged by size: 2702 1546 1339 711 BsaBI GATnn'nnATC Cuts at: 2121 6292 2121 Size: 4171 2127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3284 4105 4487 5122 Size: 53 83 186 936 1751 821 382 635 Cuts at: 5122 275 Size: 1451 Fragments arranged by size: 1751 1451 936 821 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2425 4731 5129 5403 5550 5961 2425 Size: 2306 398 274 147 411 2762 Fragments arranged by size: 2762 2306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3060 3090 1178 Size: 30 165 30 1657 30 4386 Fragments arranged by size: 4386 1657 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5412 BseYI C'CCAG_C Cuts at: 2831 5501 5997 2831 Size: 2670 496 3132 Fragments arranged by size: 3132 2670 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5725 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 3748 4509 5537 1540 Size: 275 186 37 1710 761 1028 2301 Fragments arranged by size: 2301 1710 1028 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 2012 2542 3350 3431 4359 4444 5515 2012 Size: 530 808 81 928 85 1071 2795 Fragments arranged by size: 2795 1071 928 808 530 85 81 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2512 2542 3350 3422 3431 3617 Size: 399 222 500 30 808 72 9 186 Cuts at: 3617 4359 4444 5515 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 500 399 222 186 85 72 30 9 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2615 3479 3653 844 Size: 1229 542 864 174 3489 Fragments arranged by size: 3489 1229 864 542 174 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2628 2889 4793 4967 65 Size: 1183 1380 261 1904 174 1396 Fragments arranged by size: 1904 1396 1380 1183 261 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2425 4942 5129 1700 Size: 110 220 395 2517 187 2869 Fragments arranged by size: 2869 2517 395 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 4358 5370 4358 Size: 1012 5286 BstAPI GCAn_nnn'nTGC Cuts at: 794 2516 794 Size: 1722 4576 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstXI CCAn_nnnn'nTGG Cuts at: 2346 2346 Size: 6298 BstYI r'GATC_y Cuts at: 1972 2122 2413 2548 3226 3535 3628 3995 4380 Size: 150 291 135 678 309 93 367 385 Cuts at: 4380 4397 5849 5935 5947 6293 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 678 385 367 346 309 291 150 135 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2131 513 Size: 552 1066 4680 Fragments arranged by size: 4680 1066 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 3117 3814 4608 4628 3117 Size: 697 794 20 4787 Fragments arranged by size: 4787 794 697 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2532 3745 Size: 54 1425 50 275 18 205 497 1213 Cuts at: 3745 4634 6263 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1213 889 497 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 3745 1537 Size: 275 223 1710 4090 Fragments arranged by size: 4090 1710 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 4228 1353 Size: 999 1876 3423 Fragments arranged by size: 3423 1876 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4885 5259 5405 387 Size: 1064 138 3296 374 146 1280 Fragments arranged by size: 3296 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2541 2624 3578 4361 4958 5745 Size: 393 573 336 83 954 783 597 787 Cuts at: 5745 6013 1239 Size: 268 1524 Fragments arranged by size: 1524 954 787 783 597 573 393 336 268 83 EcoNI CCTnn'n_nnAGG Cuts at: 2642 2642 Size: 6298 EcoO109I rG'GnC_Cy Cuts at: 2062 3419 2062 Size: 1357 4941 EcoRI G'AATT_C Cuts at: 2323 2805 3607 3985 2323 Size: 482 802 378 4636 Fragments arranged by size: 4636 802 482 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2405 2972 2405 Size: 567 5731 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 2028 2046 5445 1678 Size: 350 18 3399 2531 Fragments arranged by size: 3399 2531 350 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3060 3092 4939 4971 5013 Size: 32 135 32 1488 32 1847 32 42 Cuts at: 5013 5045 1373 Size: 32 2626 Fragments arranged by size: 2626 1847 1488 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3354 3897 3354 Size: 543 5755 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 5412 5596 1305 Size: 753 3354 184 2007 Fragments arranged by size: 3354 2007 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 10 Size: 54 1425 343 702 3774 Fragments arranged by size: 3774 1425 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2595 3343 4256 4615 4774 518 Size: 648 1081 348 748 913 359 159 2042 Fragments arranged by size: 2042 1081 913 748 648 359 348 159 MspA1I CmG'CkG Cuts at: 1606 2085 3714 4391 5539 5784 1606 Size: 479 1629 677 1148 245 2120 Fragments arranged by size: 2120 1629 1148 677 479 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5746 NdeI CA'TA_TG Cuts at: 387 2496 387 Size: 2109 4189 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 3134 3656 5201 1132 Size: 97 1905 522 1545 2229 Fragments arranged by size: 2229 1905 1545 522 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 2884 1108 Size: 1776 4522 PfoI T'CCnGG_A Cuts at: 2640 2640 Size: 6298 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PmlI CAC'GTG Cuts at: 2543 2543 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2062 Size: 6298 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 2894 3868 2894 Size: 974 5324 PspOMI G'GGCC_C Cuts at: 3418 3418 Size: 6298 PstI C_TGCA'G Cuts at: 830 3650 830 Size: 2820 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SfcI C'TryA_G Cuts at: 826 1045 2307 2961 3646 4781 5462 5653 826 Size: 219 1262 654 685 1135 681 191 1471 Fragments arranged by size: 1471 1262 1135 685 681 654 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 4402 5303 5565 820 Size: 197 926 354 2105 901 262 1553 Fragments arranged by size: 2105 1553 926 901 354 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 2621 152 Size: 2469 3829 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 2227 4222 5 Size: 47 2175 1995 2081 Fragments arranged by size: 2175 2081 1995 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2345 3737 513 Size: 552 1280 1392 3074 Fragments arranged by size: 3074 1392 1280 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2781 3064 4325 4342 4495 4680 5125 Size: 1255 1201 283 1261 17 153 185 445 Cuts at: 5125 325 Size: 1498 Fragments arranged by size: 1498 1261 1255 1201 445 283 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 4544 6256 Size: 274 80 33 51 1443 339 2228 1712 Cuts at: 6256 96 Size: 138 Fragments arranged by size: 2228 1712 1443 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2809 6258 6290 2777 Size: 32 3449 32 2785 Fragments arranged by size: 3449 2785 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6298 XbaI T'CTAG_A Cuts at: 3634 3634 Size: 6298 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 2949 4427 2949 Size: 1478 4820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3285 4106 276 Size: 53 83 186 936 1751 821 2468 Fragments arranged by size: 2468 1751 936 821 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI