################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FseI FspI HaeII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 1602 GACGCC G[GA]^CG[CT]C Alw44I 1632 GTGCAC G^TGCAC ApaLI 1632 GTGCAC G^TGCAC AvaI 1828 CTCGAG C^[CT]CG[GA]G BanI 571 GGCACC G^G[CT][GA]CC BbrPI 1154 CACGTG CAC^GTG BbsI 1116 GTCTTC GAAGAC(2/6) BciVI 1909 GGATAC GTATCC(6/5) BclI 623 TGATCA T^GATCA BcnI 2145 CCCGG CC^[GC]GG BglII 1393 AGATCT A^GATCT BpuAI 1116 GTCTTC GAAGAC(2/6) BsaAI 1154 CACGTG [CT]AC^GT[GA] BsaHI 1602 GACGCC G[GA]^CG[CT]C BsaI 2103 GGTCTC GGTCTC(1/5) BseRI 592 GAGGAG GAGGAG(10/8) BsmBI 1110 GAGACG CGTCTC(1/5) BsmI 1437 GAATGC GAATGC(1/-1) BsoBI 1828 CTCGAG C^[CT]CG[GA]G BspLU11I 725 ACATGT A^CATGT BsrFI 1036 ACCGGC [GA]^CCGG[CT] Bst1107I 287 GTATAC GTA^TAC BstXI 2379 CCAGCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 287 GTATAC GTA^TAC CfoI 1240 GCGC GCG^C Cfr10I 1036 ACCGGC [GA]^CCGG[CT] DraIII 1926 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2456 TGGCCG [CT]^GGCC[GA] EcoRI 1284 GAATTC G^AATTC FbaI 623 TGATCA T^GATCA HhaI 1240 GCGC GCG^C Hin1I 1602 GACGCC G[GA]^CG[CT]C HinP1I 1240 GCGC G^CGC HindIII 1740 AAGCTT A^AGCTT MfeI 2018 CAATTG C^AATTG MslI 1591 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 2018 CAATTG C^AATTG NciI 2145 CCCGG CC^[GC]GG NdeI 502 CATATG CA^TATG PaeR7I 1828 CTCGAG C^TCGAG PflMI 1473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1154 CACGTG CAC^GTG PmlI 1154 CACGTG CAC^GTG PpuMI 1862 AGGACCT [GA]G^G[AT]CC[CT] SalI 461 GTCGAC G^TCGAC SphI 1823 GCATGC GCATG^C Van91I 1473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1828 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 287 GTATAC GT^[AC][GT]AC 461 GTCGAC AflIII 725 ACATGT A^C[GA][CT]GT 1153 ACACGT BanII 210 GAGCCC G[GA]GC[CT]^C 1620 GAGCCC BfaI 490 CTAG C^TAG 859 CTAG BlpI 1312 GCTCAGC GC^T[ACGT]AGC 2189 GCTCAGC Bpu1102I 1312 GCTCAGC GC^T[ACGT]AGC 2189 GCTCAGC BsgI 1289 CTGCAC GTGCAG(16/14) 2242 GTGCAG BspMI 1571 ACCTGC ACCTGC(4/8) 2023 GCAGGT CelII 1312 GCTCAGC GC^T[ACGT]AGC 2189 GCTCAGC DraI 922 TTTAAA TTT^AAA 1855 TTTAAA DraII 1541 AGGCCCC [GA]G^G[ACGT]CC[CT] 1862 AGGACCT DrdI 1455 GACAGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2356 GACTCACCAGTC DsaI 166 CCGTGG C^C[GA][CT]GG 2117 CCGTGG EcoNI 1533 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1724 CCTCTACCAGG EcoO109I 1541 AGGCCCC [GA]G^G[ACGT]CC[CT] 1862 AGGACCT EcoRV 383 GATATC GAT^ATC 475 GATATC HapII 1037 CCGG C^CGG 2146 CCGG HgaI 464 GACGC GACGC(5/10) 1602 GACGC HincII 461 GTCGAC GT[CT]^[GA]AC 837 GTCAAC HindII 461 GTCGAC GT[CT]^[GA]AC 837 GTCAAC HpaII 1037 CCGG C^CGG 2146 CCGG MaeI 490 CTAG C^TAG 859 CTAG MspA1I 1384 CAGCTG C[AC]G^C[GT]G 1793 CAGCTG MspI 1037 CCGG C^CGG 2146 CCGG MwoI 1312 GCTCAGCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 2504 GCTCATCCTGC NspI 725 ACATGT [GA]CATG^[CT] 1823 GCATGC PstI 1573 CTGCAG CTGCA^G 2212 CTGCAG PvuII 1384 CAGCTG CAG^CTG 1793 CAGCTG SapI 579 GAAGAGC GCTCTTC(1/4) 1345 GAAGAGC SfcI 1573 CTGCAG C^T[GA][CT]AG 2212 CTGCAG StuI 1614 AGGCCT AGG^CCT 1732 AGGCCT XcmI 105 CCAGCCTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1021 CCAGCTTTCAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII