(Circular) (Six-base) MAPSORT of: pFN21AE2337.seq Check: 4892 from: 1 to: 7306 pFN21AE2337 7306 bp With 182 enzymes: SgfI * April 13, 2011 11:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4019 4019 Size: 7306 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5116 278 Size: 53 83 186 936 3580 2468 Fragments arranged by size: 3580 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2293 2467 4649 1937 Size: 356 174 2182 4594 Fragments arranged by size: 4594 2182 356 174 Acc65I G'GTAC_C Cuts at: 4627 4627 Size: 7306 AclI AA'CG_TT Cuts at: 1563 5433 5806 1563 Size: 3870 373 3063 Fragments arranged by size: 3870 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3537 5369 6753 7021 1632 Size: 1905 1832 1384 268 1917 Fragments arranged by size: 1917 1905 1832 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7109 AflIII A'CryG_T Cuts at: 1203 2730 3158 6205 7182 1203 Size: 1527 428 3047 977 1327 Fragments arranged by size: 3047 1527 1327 977 428 AgeI A'CCGG_T Cuts at: 6137 6137 Size: 7306 AhdI GACnn_n'nnGTC Cuts at: 6035 6035 Size: 7306 AleI CACnn'nnGTG Cuts at: 1166 3600 1166 Size: 2434 4872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4272 1184 Size: 3088 4218 AlwNI CAG_nnn'CTG Cuts at: 2774 3484 4225 4406 5049 6621 2774 Size: 710 741 181 643 1572 3459 Fragments arranged by size: 3459 1572 741 710 643 181 ApaLI G'TGCA_C Cuts at: 3637 5363 6519 3637 Size: 1726 1156 4424 Fragments arranged by size: 4424 1726 1156 ApoI r'AATT_y Cuts at: 1493 2318 2759 2819 3238 3289 3857 4615 4841 Size: 825 441 60 419 51 568 758 226 Cuts at: 4841 4993 7102 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 825 758 568 441 419 226 152 60 51 AseI AT'TA_AT Cuts at: 160 1032 5860 160 Size: 872 4828 1606 Fragments arranged by size: 4828 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7306 AvaI C'yCGr_G Cuts at: 1870 1943 3833 4631 1870 Size: 73 1890 798 4545 Fragments arranged by size: 4545 1890 798 73 BamHI G'GATC_C Cuts at: 4636 5003 4636 Size: 367 6939 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2576 4627 6082 618 Size: 325 217 627 789 2051 1455 1842 Fragments arranged by size: 2051 1842 1455 789 627 325 217 BanII G_rGCy'C Cuts at: 1391 2219 3629 4625 1391 Size: 828 1410 996 4072 Fragments arranged by size: 4072 1410 996 828 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3114 928 Size: 971 1215 5120 Fragments arranged by size: 5120 1215 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5497 5531 1903 Size: 34 3560 34 3678 Fragments arranged by size: 3678 3560 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5198 6408 3908 Size: 1290 1210 4806 Fragments arranged by size: 4806 1290 1210 BclI T'GATC_A Cuts at: 1548 2628 1548 Size: 1080 6226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5917 136 Size: 107 122 71 631 605 4245 1525 Fragments arranged by size: 4245 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3398 7301 3398 Size: 3903 3403 BlpI GC'TnA_GC Cuts at: 3318 4195 4723 3318 Size: 877 528 5901 Fragments arranged by size: 5901 877 528 Bme1580I G_kGCm'C Cuts at: 1790 3531 3641 4420 5367 6523 1790 Size: 1741 110 779 947 1156 2573 Fragments arranged by size: 2573 1741 1156 947 779 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7306 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3074 3243 3431 Size: 416 53 83 399 24 1657 169 188 Cuts at: 3431 5037 5995 442 Size: 1606 958 1753 Fragments arranged by size: 1753 1657 1606 958 416 399 188 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2772 3058 3822 5966 1239 Size: 1092 441 286 764 2144 2579 Fragments arranged by size: 2579 2144 1092 764 441 286 Bpu10I CC'TnA_GC Cuts at: 3623 3623 Size: 7306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3573 3967 5431 6296 6594 3573 Size: 394 1464 865 298 4285 Fragments arranged by size: 4285 1464 865 394 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4114 5969 882 Size: 617 2615 1855 2219 Fragments arranged by size: 2615 2219 1855 617 BsaAI yAC'GTr Cuts at: 493 1204 3161 5097 493 Size: 711 1957 1936 2702 Fragments arranged by size: 2702 1957 1936 711 BsaBI GATnn'nnATC Cuts at: 7300 7300 Size: 7306 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3608 5113 5495 6130 Size: 53 83 186 936 2075 1505 382 635 Cuts at: 6130 275 Size: 1451 Fragments arranged by size: 2075 1505 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5739 6137 6411 6558 6969 5739 Size: 398 274 147 411 6076 Fragments arranged by size: 6076 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2591 2621 1178 Size: 30 165 30 1188 30 5863 Fragments arranged by size: 5863 1188 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 1186 Size: 1426 5880 BseYI C'CCAG_C Cuts at: 3506 6509 7005 3506 Size: 3003 496 3807 Fragments arranged by size: 3807 3003 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3279 4268 1163 Size: 573 1543 989 4201 Fragments arranged by size: 4201 1543 989 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4756 5517 6545 1540 Size: 275 186 2755 761 1028 2301 Fragments arranged by size: 2755 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3641 3941 3991 5367 5452 6523 3641 Size: 300 50 1376 85 1071 4424 Fragments arranged by size: 4424 1376 1071 300 85 50 BsmI GAATG_Cn' Cuts at: 3448 4817 4910 3448 Size: 1369 93 5844 Fragments arranged by size: 5844 1369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3109 3109 Size: 7306 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 3531 3629 3641 3941 3991 4420 Size: 399 429 1312 98 12 300 50 429 Cuts at: 4420 4625 5367 5452 6523 1391 Size: 205 742 85 1071 2174 Fragments arranged by size: 2174 1312 1071 742 429 429 399 300 205 98 85 50 12 BspEI T'CCGG_A Cuts at: 6969 6969 Size: 7306 BspHI T'CATG_A Cuts at: 5195 6925 5195 Size: 1730 5576 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3585 4019 4661 844 Size: 2741 434 642 3489 Fragments arranged by size: 3489 2741 642 434 BsrBI CCG'CTC Cuts at: 1394 1823 5193 1394 Size: 429 3370 3507 Fragments arranged by size: 3507 3370 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5801 5975 65 Size: 1183 4553 174 1396 Fragments arranged by size: 4553 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3041 5950 6137 1700 Size: 110 1231 2909 187 2869 Fragments arranged by size: 2909 2869 1231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7306 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7306 BssSI C'ACGA_G Cuts at: 5366 6378 5366 Size: 1012 6294 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7306 BstBI TT'CG_AA Cuts at: 6110 6110 Size: 7306 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7306 BstXI CCAn_nnnn'nTGG Cuts at: 4391 4391 Size: 7306 BstYI r'GATC_y Cuts at: 1972 2106 2223 2980 3398 4636 5003 5388 5405 Size: 134 117 757 418 1238 367 385 17 Cuts at: 5405 6857 6943 6955 7301 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1238 757 418 385 367 346 134 117 86 17 12 BstZ17I GTA'TAC Cuts at: 2294 2294 Size: 7306 Bsu36I CC'TnA_GG Cuts at: 2277 3543 4549 2277 Size: 1266 1006 5034 Fragments arranged by size: 5034 1266 1006 BtgI C'CryG_G Cuts at: 513 1065 2004 2171 4122 513 Size: 552 939 167 1951 3697 Fragments arranged by size: 3697 1951 939 552 167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6567 Fragments arranged by size: 6567 683 56 BtsI GCAGTG_nn' Cuts at: 3531 3941 4059 4822 5616 5636 3531 Size: 410 118 763 794 20 5201 Fragments arranged by size: 5201 794 763 410 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7306 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2248 2283 4104 4139 529 Size: 35 1684 35 1821 35 3696 Fragments arranged by size: 3696 1821 1684 35 35 35 DraI TTT'AAA Cuts at: 2929 3862 4610 2929 Size: 933 748 5625 Fragments arranged by size: 5625 933 748 DraIII CAC_nnn'GTG Cuts at: 3936 3936 Size: 7306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3466 4367 6313 809 Size: 507 2150 901 1946 1802 Fragments arranged by size: 2150 1946 1802 901 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4461 4753 5642 Size: 54 1425 50 275 18 2631 292 889 Cuts at: 5642 7271 8 Size: 1629 43 Fragments arranged by size: 2631 1629 1425 889 292 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4753 1537 Size: 275 2941 4090 Fragments arranged by size: 4090 2941 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2579 3345 3655 5236 1353 Size: 1226 766 310 1581 3423 Fragments arranged by size: 3423 1581 1226 766 310 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5893 6267 6413 387 Size: 1064 138 4304 374 146 1280 Fragments arranged by size: 4304 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2772 3058 3537 3822 5369 5966 Size: 393 699 441 286 479 285 1547 597 Cuts at: 5966 6753 7021 1239 Size: 787 268 1524 Fragments arranged by size: 1547 1524 787 699 597 479 441 393 286 285 268 EcoNI CCTnn'n_nnAGG Cuts at: 3542 3733 3542 Size: 191 7115 EcoO109I rG'GnC_Cy Cuts at: 3547 3868 3547 Size: 321 6985 EcoRI G'AATT_C Cuts at: 3289 4615 4993 3289 Size: 1326 378 5602 Fragments arranged by size: 5602 1326 378 EcoRV GAT'ATC Cuts at: 2390 2482 2390 Size: 92 7214 FspI TGC'GCA Cuts at: 5812 5812 Size: 7306 HaeII r_GCGC'y Cuts at: 1678 6453 1678 Size: 4775 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2544 2576 3526 3558 3906 Size: 32 135 32 972 32 950 32 348 Cuts at: 3906 3938 5947 5979 6021 6053 1373 Size: 32 2009 32 42 32 2626 Fragments arranged by size: 2626 2009 972 950 348 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2468 2844 4650 4896 669 Size: 988 281 530 376 1806 246 3079 Fragments arranged by size: 3079 1806 988 530 376 281 246 HindIII A'AGCT_T Cuts at: 748 3745 748 Size: 2997 4309 HpaI GTT'AAC Cuts at: 4896 4896 Size: 7306 KpnI G_GTAC'C Cuts at: 4631 4631 Size: 7306 MfeI C'AATT_G Cuts at: 4023 4905 4023 Size: 882 6424 MluI A'CGCG_T Cuts at: 7182 7182 Size: 7306 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6420 6604 1305 Size: 5115 184 2007 Fragments arranged by size: 5115 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5484 Fragments arranged by size: 5484 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3600 5264 5623 5782 518 Size: 648 2434 1664 359 159 2042 Fragments arranged by size: 2434 2042 1664 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3391 3800 4722 5399 6547 6792 1606 Size: 1785 409 922 677 1148 245 2120 Fragments arranged by size: 2120 1785 1148 922 677 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5815 Fragments arranged by size: 5815 939 552 NdeI CA'TA_TG Cuts at: 387 2508 387 Size: 2121 5185 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7306 NotI GC'GGCC_GC Cuts at: 4753 4753 Size: 7306 NspI r_CATG'y Cuts at: 1132 1229 2734 3832 4664 6209 1132 Size: 97 1505 1098 832 1545 2229 Fragments arranged by size: 2229 1545 1505 1098 832 97 PasI CC'CwG_GG Cuts at: 1348 3484 3946 4265 1348 Size: 2136 462 319 4389 Fragments arranged by size: 4389 2136 462 319 PciI A'CATG_T Cuts at: 2730 6205 2730 Size: 3475 3831 PflMI CCAn_nnn'nTGG Cuts at: 1108 3484 1108 Size: 2376 4930 PmeI GTTT'AAAC Cuts at: 4610 4610 Size: 7306 PmlI CAC'GTG Cuts at: 3161 3161 Size: 7306 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5375 5407 6888 6920 4178 Size: 32 1165 32 1481 32 4564 Fragments arranged by size: 4564 1481 1165 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3868 3868 Size: 7306 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7306 PsiI TTA'TAA Cuts at: 4876 4876 Size: 7306 PspXI vC'TCGA_Gb Cuts at: 3833 3833 Size: 7306 PstI C_TGCA'G Cuts at: 830 3582 4221 4658 830 Size: 2752 639 437 3478 Fragments arranged by size: 3478 2752 639 437 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7306 PvuII CAG'CTG Cuts at: 3391 3800 3391 Size: 409 6897 SalI G'TCGA_C Cuts at: 1936 2466 4648 1936 Size: 530 2182 4594 Fragments arranged by size: 4594 2182 530 SapI GCTCTTCn'nnn_ Cuts at: 2579 3345 2579 Size: 766 6540 SbfI CC_TGCA'GG Cuts at: 4658 4658 Size: 7306 ScaI AGT'ACT Cuts at: 5554 7266 5554 Size: 1712 5594 SfcI C'TryA_G Cuts at: 826 1045 3578 4217 4654 5789 6470 6661 826 Size: 219 2533 639 437 1135 681 191 1471 Fragments arranged by size: 2533 1471 1135 681 639 437 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7306 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7306 SmaI CCC'GGG Cuts at: 1872 4633 1872 Size: 2761 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3588 3833 3982 5410 6311 6573 Size: 197 926 1645 245 149 1428 901 262 Cuts at: 6573 820 Size: 1553 Fragments arranged by size: 1645 1553 1428 926 901 262 245 197 149 SnaBI TAC'GTA Cuts at: 493 5097 493 Size: 4604 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7306 SphI G_CATG'C Cuts at: 1132 3832 4664 1132 Size: 2700 832 3774 Fragments arranged by size: 3774 2700 832 SspI AAT'ATT Cuts at: 5 52 5230 5 Size: 47 5178 2081 Fragments arranged by size: 5178 2081 47 StuI AGG'CCT Cuts at: 1517 3621 3739 1517 Size: 2104 118 5084 Fragments arranged by size: 5084 2104 118 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 2572 3616 4745 513 Size: 552 939 144 424 1044 1129 3074 Fragments arranged by size: 3074 1129 1044 939 552 424 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2739 3201 3659 4255 4456 5333 5350 Size: 1255 1159 462 458 596 201 877 17 Cuts at: 5350 5503 5688 6133 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1159 877 596 462 458 445 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3385 5552 7264 Size: 274 80 33 51 1443 1408 2167 1712 Cuts at: 7264 96 Size: 138 Fragments arranged by size: 2167 1712 1443 1408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7266 7298 7266 Size: 32 7274 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7306 XbaI T'CTAG_A Cuts at: 4642 4642 Size: 7306 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 3033 2117 Size: 916 6390 XhoI C'TCGA_G Cuts at: 1943 3833 1943 Size: 1890 5416 XmaI C'CCGG_G Cuts at: 1870 4631 1870 Size: 2761 4545 XmnI GAAnn'nnTTC Cuts at: 5435 5435 Size: 7306 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5114 276 Size: 53 83 186 936 3580 2468 Fragments arranged by size: 3580 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI