Restriction map for
pFN21AE2337
(Linear) (Six-base) MAPSORT of: KIEE2337.seq Check: 4051 from: 1 to: 2601
KIEE2337.orf
With 182 enzymes: SgfI *
April 12, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2014 2601
Size: 2014 587
AccI GT'mk_AC
Cuts at: 0 288 462 2601
Size: 288 174 2139
Fragments arranged by size:
2139 288 174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1532 2601
Size: 1532 1069
AflIII A'CryG_T
Cuts at: 0 725 1153 2601
Size: 725 428 1448
Fragments arranged by size:
1448 725 428
AleI CACnn'nnGTG
Cuts at: 0 1595 2601
Size: 1595 1006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2267 2601
Size: 2267 334
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1479 2220 2401 2601
Size: 769 710 741 181 200
Fragments arranged by size:
769 741 710 200 181
ApaLI G'TGCA_C
Cuts at: 0 1632 2601
Size: 1632 969
ApoI r'AATT_y
Cuts at: 0 313 754 814 1233 1284 1852 2601
Size: 313 441 60 419 51 568 749
Fragments arranged by size:
749 568 441 419 313 60 51
AvaI C'yCGr_G
Cuts at: 0 1828 2601
Size: 1828 773
BanI G'GyrC_C
Cuts at: 0 571 2601
Size: 571 2030
BanII G_rGCy'C
Cuts at: 0 214 1624 2601
Size: 214 1410 977
Fragments arranged by size:
1410 977 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 1109 2601
Size: 1109 1492
BciVI GTATCCnnnnn_n'
Cuts at: 0 1903 2601
Size: 1903 698
BclI T'GATC_A
Cuts at: 0 623 2601
Size: 623 1978
BglII A'GATC_T
Cuts at: 0 1393 2601
Size: 1393 1208
BlpI GC'TnA_GC
Cuts at: 0 1313 2190 2601
Size: 1313 877 411
Fragments arranged by size:
1313 877 411
Bme1580I G_kGCm'C
Cuts at: 0 1526 1636 2415 2601
Size: 1526 110 779 186
Fragments arranged by size:
1526 779 186 110
BmrI ACTGGGnnnn_n'
Cuts at: 0 1069 1238 1426 2601
Size: 1069 169 188 1175
Fragments arranged by size:
1175 1069 188 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 767 1053 1817 2601
Size: 326 441 286 764 784
Fragments arranged by size:
784 764 441 326 286
Bpu10I CC'TnA_GC
Cuts at: 0 1618 2601
Size: 1618 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1962 2601
Size: 1568 394 639
Fragments arranged by size:
1568 639 394
BsaI GGTCTCn'nnnn_
Cuts at: 0 2109 2601
Size: 2109 492
BsaAI yAC'GTr
Cuts at: 0 1156 2601
Size: 1156 1445
BsaHI Gr'CG_yC
Cuts at: 0 1603 2601
Size: 1603 998
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 2601
Size: 586 30 1985
Fragments arranged by size:
1985 586 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 2601
Size: 607 1994
BseYI C'CCAG_C
Cuts at: 0 1501 2601
Size: 1501 1100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1274 2263 2601
Size: 1274 989 338
Fragments arranged by size:
1274 989 338
BsiHKAI G_wGCw'C
Cuts at: 0 1636 1936 1986 2601
Size: 1636 300 50 615
Fragments arranged by size:
1636 615 300 50
BsmI GAATG_Cn'
Cuts at: 0 1443 2601
Size: 1443 1158
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1104 2601
Size: 1104 1497
Bsp1286I G_dGCh'C
Cuts at: 0 214 1526 1624 1636 1936 1986 2415 2601
Size: 214 1312 98 12 300 50 429 186
Fragments arranged by size:
1312 429 300 214 186 98 50 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1580 2014 2601
Size: 1580 434 587
Fragments arranged by size:
1580 587 434
BsrFI r'CCGG_y
Cuts at: 0 1036 2601
Size: 1036 1565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2386 2601
Size: 2386 215
BstYI r'GATC_y
Cuts at: 0 101 218 975 1393 2601
Size: 101 117 757 418 1208
Fragments arranged by size:
1208 757 418 117 101
BstZ17I GTA'TAC
Cuts at: 0 289 2601
Size: 289 2312
Bsu36I CC'TnA_GG
Cuts at: 0 272 1538 2544 2601
Size: 272 1266 1006 57
Fragments arranged by size:
1266 1006 272 57
BtgI C'CryG_G
Cuts at: 0 166 2117 2601
Size: 166 1951 484
Fragments arranged by size:
1951 484 166
BtsI GCAGTG_nn'
Cuts at: 0 1526 1936 2054 2601
Size: 1526 410 118 547
Fragments arranged by size:
1526 547 410 118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 243 278 2099 2134 2601
Size: 243 35 1821 35 467
Fragments arranged by size:
1821 467 243 35 35
DraI TTT'AAA
Cuts at: 0 924 1857 2601
Size: 924 933 744
Fragments arranged by size:
933 924 744
DraIII CAC_nnn'GTG
Cuts at: 0 1931 2601
Size: 1931 670
DrdI GACnn_nn'nnGTC
Cuts at: 0 1461 2362 2601
Size: 1461 901 239
Fragments arranged by size:
1461 901 239
EaeI y'GGCC_r
Cuts at: 0 2456 2601
Size: 2456 145
EarI CTCTTCn'nnn_
Cuts at: 0 574 1340 1650 2601
Size: 574 766 310 951
Fragments arranged by size:
951 766 574 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 767 1053 1532 1817 2601
Size: 326 441 286 479 285 784
Fragments arranged by size:
784 479 441 326 286 285
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1537 1728 2601
Size: 1537 191 873
Fragments arranged by size:
1537 873 191
EcoO109I rG'GnC_Cy
Cuts at: 0 1542 1863 2601
Size: 1542 321 738
Fragments arranged by size:
1542 738 321
EcoRI G'AATT_C
Cuts at: 0 1284 2601
Size: 1284 1317
EcoRV GAT'ATC
Cuts at: 0 385 477 2601
Size: 385 92 2124
Fragments arranged by size:
2124 385 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 539 571 1521 1553 1901 1933 2601
Size: 539 32 950 32 348 32 668
Fragments arranged by size:
950 668 539 348 32 32 32
HincII GTy'rAC
Cuts at: 0 463 839 2601
Size: 463 376 1762
Fragments arranged by size:
1762 463 376
HindIII A'AGCT_T
Cuts at: 0 1740 2601
Size: 1740 861
MfeI C'AATT_G
Cuts at: 0 2018 2601
Size: 2018 583
MslI CAynn'nnrTG
Cuts at: 0 1595 2601
Size: 1595 1006
MspA1I CmG'CkG
Cuts at: 0 1386 1795 2601
Size: 1386 409 806
Fragments arranged by size:
1386 806 409
NdeI CA'TA_TG
Cuts at: 0 503 2601
Size: 503 2098
NspI r_CATG'y
Cuts at: 0 729 1827 2601
Size: 729 1098 774
Fragments arranged by size:
1098 774 729
PasI CC'CwG_GG
Cuts at: 0 1479 1941 2260 2601
Size: 1479 462 319 341
Fragments arranged by size:
1479 462 341 319
PciI A'CATG_T
Cuts at: 0 725 2601
Size: 725 1876
PflMI CCAn_nnn'nTGG
Cuts at: 0 1479 2601
Size: 1479 1122
PmlI CAC'GTG
Cuts at: 0 1156 2601
Size: 1156 1445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2173 2205 2601
Size: 2173 32 396
Fragments arranged by size:
2173 396 32
PpuMI rG'GwC_Cy
Cuts at: 0 1863 2601
Size: 1863 738
PspXI vC'TCGA_Gb
Cuts at: 0 1828 2601
Size: 1828 773
PstI C_TGCA'G
Cuts at: 0 1577 2216 2601
Size: 1577 639 385
Fragments arranged by size:
1577 639 385
PvuII CAG'CTG
Cuts at: 0 1386 1795 2601
Size: 1386 409 806
Fragments arranged by size:
1386 806 409
SalI G'TCGA_C
Cuts at: 0 461 2601
Size: 461 2140
SapI GCTCTTCn'nnn_
Cuts at: 0 574 1340 2601
Size: 574 766 1261
Fragments arranged by size:
1261 766 574
SfcI C'TryA_G
Cuts at: 0 1573 2212 2601
Size: 1573 639 389
Fragments arranged by size:
1573 639 389
SmlI C'TyrA_G
Cuts at: 0 1583 1828 1977 2601
Size: 1583 245 149 624
Fragments arranged by size:
1583 624 245 149
SphI G_CATG'C
Cuts at: 0 1827 2601
Size: 1827 774
StuI AGG'CCT
Cuts at: 0 1616 1734 2601
Size: 1616 118 867
Fragments arranged by size:
1616 867 118
StyI C'CwwG_G
Cuts at: 0 143 567 1611 2601
Size: 143 424 1044 990
Fragments arranged by size:
1044 990 424 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 734 1196 1654 2250 2451 2601
Size: 734 462 458 596 201 150
Fragments arranged by size:
734 596 462 458 201 150
TatI w'GTAC_w
Cuts at: 0 1380 2601
Size: 1380 1221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 1028 2601
Size: 112 916 1573
Fragments arranged by size:
1573 916 112
XhoI C'TCGA_G
Cuts at: 0 1828 2601
Size: 1828 773
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI CspCI DraI DraIII DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII MfeI
MslI MspA1I NdeI NspI PasI PciI PflMI PmlI
PpiI PpuMI PspXI PstI PvuII SalI SapI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI EagI
EciI EcoICRI FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XmaI XmnI ZraI