Restriction map for
pFN21AE5422
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 738 GTATAC GT^[AC][GT]AC
AciI 888 GCGG CCGC(-3/-1)
AfaI 218 GTAC GT^AC
Alw44I 808 GTGCAC G^TGCAC
ApaLI 808 GTGCAC G^TGCAC
AspHI 808 GTGCAC G[AT]GC[AT]^C
BamHI 781 GGATCC G^GATCC
BanI 85 GGTGCC G^G[CT][GA]CC
BbsI 633 GAAGAC GAAGAC(2/6)
BciVI 869 GTATCC GTATCC(6/5)
BpmI 264 CTCCAG CTGGAG(16/14)
BpuAI 633 GAAGAC GAAGAC(2/6)
BsgI 956 GTGCAG GTGCAG(16/14)
BsiHKAI 808 GTGCAC G[AT]GC[AT]^C
BsmAI 898 GTCTC GTCTC(1/5)
BspMI 286 GCAGGT ACCTGC(4/8)
BssKI 324 CCTGG ^CC[ACGT]GG
BssSI 467 CTCGTG CACGAG(-5/-1)
Bst1107I 738 GTATAC GTA^TAC
BstF5I 42 GGATG GGATG(2/0)
BstNI 324 CCTGG CC^[AT]GG
BstZ17I 738 GTATAC GTA^TAC
DraII 938 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 951 GAAGAG CTCTTC(1/4)
EcoNI 646 CCTAAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 938 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 924 GAATTC G^AATTC
EcoRII 324 CCTGG ^CC[AT]GG
FokI 42 GGATG GGATG(9/13)
HaeIII 939 GGCC GG^CC
HincII 3 GTCAAC GT[CT]^[GA]AC
HindII 3 GTCAAC GT[CT]^[GA]AC
Ksp632I 951 GAAGAG CTCTTC(1/4)
MaeII 333 ACGT A^CGT
MvaI 324 CCTGG CC^[AT]GG
MwoI 619 GCTGGTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RsaI 218 GTAC GT^AC
ScaI 217 AGTACT AGT^ACT
ScrFI 324 CCTGG CC^[ACGT]GG
Tsp45I 280 GTCAC ^GT[GC]AC
XbaI 492 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 395 AAATTC [GA]^AATT[CT]
924 GAATTC
ApoI 395 AAATTC [GA]^AATT[CT]
924 GAATTC
Asp700I 656 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
856 GAACTCTTTC
AvaII 327 GGTCC G^G[AT]CC
642 GGACC
BfaI 493 CTAG C^TAG
587 CTAG
BmyI 592 GTGCCC G[AGT]GC[ACT]^C
808 GTGCAC
BseRI 931 GAGGAG GAGGAG(10/8)
934 GAGGAG
BsiYI 318 CCTTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
646 CCTAAATTAGG
BslI 318 CCTTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
646 CCTAAATTAGG
Bsp1286I 592 GTGCCC G[AGT]GC[ACT]^C
808 GTGCAC
DsaI 555 CCATGG C^C[GA][CT]GG
824 CCATGG
EcoT14I 555 CCATGG C^C[AT][AT]GG
824 CCATGG
HphI 580 GGTGA GGTGA(8/7)
609 GGTGA
MaeI 493 CTAG C^TAG
587 CTAG
MseI 830 TTAA T^TAA
917 TTAA
NcoI 555 CCATGG C^CATGG
824 CCATGG
PleI 496 GACTC GAGTC(4/5)
636 GACTC
SspI 973 AATATT AAT^ATT
990 AATATT
StyI 555 CCATGG C^C[AT][AT]GG
824 CCATGG
TfiI 583 GATTC G^A[AT]TC
661 GATTC
Tru9I 830 TTAA T^TAA
917 TTAA
XmnI 656 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
856 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsrI
BstYI Cfr13I DdeI DpnII Fnu4HI
HinfI ItaI MaeIII MboI MboII
MflI MnlI NdeII NlaIII NlaIV
Sau3AI Sau96I TseI Tsp509I TspRI
XhoII