Restriction map for
pFN21AE5422
(Linear) (Six-base) MAPSORT of: KIEE5422.seq Check: 8679 from: 1 to: 996
KIEE5422.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 277 996
Size: 277 719
AccI GT'mk_AC
Cuts at: 0 739 996
Size: 739 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 178 193 652 751 996
Size: 142 36 15 459 99 245
Fragments arranged by size:
459 245 142 99 36 15
ApaLI G'TGCA_C
Cuts at: 0 808 996
Size: 808 188
ApoI r'AATT_y
Cuts at: 0 395 924 996
Size: 395 529 72
Fragments arranged by size:
529 395 72
BamHI G'GATC_C
Cuts at: 0 781 996
Size: 781 215
BanI G'GyrC_C
Cuts at: 0 85 996
Size: 85 911
BbsI GAAGACnn'nnnn_
Cuts at: 0 640 996
Size: 640 356
BciVI GTATCCnnnnn_n'
Cuts at: 0 880 996
Size: 880 116
Bme1580I G_kGCm'C
Cuts at: 0 596 812 996
Size: 596 216 184
Fragments arranged by size:
596 216 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 808 996
Size: 51 757 188
Fragments arranged by size:
757 188 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 996
Size: 249 747
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 949 996
Size: 946 3 47
Fragments arranged by size:
946 47 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 996
Size: 977 19
BsiHKAI G_wGCw'C
Cuts at: 0 812 996
Size: 812 184
Bsp1286I G_dGCh'C
Cuts at: 0 596 812 996
Size: 596 216 184
Fragments arranged by size:
596 216 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 277 996
Size: 277 719
BssSI C'ACGA_G
Cuts at: 0 467 996
Size: 467 529
BstYI r'GATC_y
Cuts at: 0 189 771 781 984 996
Size: 189 582 10 203 12
Fragments arranged by size:
582 203 189 12 10
BstZ17I GTA'TAC
Cuts at: 0 740 996
Size: 740 256
BtgI C'CryG_G
Cuts at: 0 555 824 996
Size: 555 269 172
Fragments arranged by size:
555 269 172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 398 433 996
Size: 398 35 563
Fragments arranged by size:
563 398 35
EarI CTCTTCn'nnn_
Cuts at: 0 946 996
Size: 946 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 903 996
Size: 903 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 178 193 249 652 751 996
Size: 142 36 15 56 403 99 245
Fragments arranged by size:
403 245 142 99 56 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 650 996
Size: 650 346
EcoO109I rG'GnC_Cy
Cuts at: 0 939 996
Size: 939 57
EcoRI G'AATT_C
Cuts at: 0 924 996
Size: 924 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 996
Size: 37 32 927
Fragments arranged by size:
927 37 32
HincII GTy'rAC
Cuts at: 0 5 996
Size: 5 991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 354 541 996
Size: 57 297 187 455
Fragments arranged by size:
455 297 187 57
NcoI C'CATG_G
Cuts at: 0 555 824 996
Size: 555 269 172
Fragments arranged by size:
555 269 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 848 880 996
Size: 848 32 116
Fragments arranged by size:
848 116 32
ScaI AGT'ACT
Cuts at: 0 219 996
Size: 219 777
SspI AAT'ATT
Cuts at: 0 975 992 996
Size: 975 17 4
Fragments arranged by size:
975 17 4
StyI C'CwwG_G
Cuts at: 0 555 824 996
Size: 555 269 172
Fragments arranged by size:
555 269 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 523 827 996
Size: 523 304 169
Fragments arranged by size:
523 304 169
TatI w'GTAC_w
Cuts at: 0 217 996
Size: 217 779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 435 467 749 781 802 834 996
Size: 435 32 282 32 21 32 162
Fragments arranged by size:
435 282 162 32 32 32 21
XbaI T'CTAG_A
Cuts at: 0 492 996
Size: 492 504
XmnI GAAnn'nnTTC
Cuts at: 0 660 860 996
Size: 660 200 136
Fragments arranged by size:
660 200 136
Enzymes that do cut:
AarI AccI AcuI ApaLI ApoI BamHI BanI BbsI
BciVI Bme1580I BmrI BpmI BseRI BsgI BsiHKAI Bsp1286I
BspMI BssSI BstYI BstZ17I BtgI CspCI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MmeI NcoI
PpiI ScaI SspI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI ZraI