Restriction map for
pFN21AE1909
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HaeIII HapII Hin1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 671 GTAC GT^AC
AlwNI 1225 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 307 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 538 GAGCTC G[AT]GC[AT]^C
AspI 412 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 345 GGACC G^G[AT]CC
BanII 538 GAGCTC G[GA]GC[CT]^C
BbsI 7 GAAGAC GAAGAC(2/6)
BmyI 538 GAGCTC G[AGT]GC[ACT]^C
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaI 473 GAGACC GGTCTC(1/5)
BsgI 753 CTGCAC GTGCAG(16/14)
BsiHKAI 538 GAGCTC G[AT]GC[AT]^C
Bsp1286I 538 GAGCTC G[AGT]GC[ACT]^C
BstXI 870 CCAAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 896 GCGC GCG^C
Cfr13I 345 GGACC G^G[ACGT]CC
DraI 660 TTTAAA TTT^AAA
EcoRI 493 GAATTC G^AATTC
EcoRV 614 GATATC GAT^ATC
EcoT14I 316 CCTTGG C^C[AT][AT]GG
FauI 834 CCCGC CCCGC(4/6)
HaeII 895 GGCGCT [GA]GCGC^[CT]
HgaI 1182 GCGTC GACGC(5/10)
HhaI 896 GCGC GCG^C
HinP1I 896 GCGC G^CGC
HincII 190 GTTGAC GT[CT]^[GA]AC
HindII 190 GTTGAC GT[CT]^[GA]AC
HphI 709 TCACC GGTGA(8/7)
MslI 247 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 445 GCGGACTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 523 GGAACC GG[ACGT]^[ACGT]CC
PflFI 412 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1270 CTGCAG CTGCA^G
RsaI 671 GTAC GT^AC
SacI 538 GAGCTC GAGCT^C
SapI 47 GAAGAGC GCTCTTC(1/4)
Sau96I 345 GGACC G^G[ACGT]CC
SfcI 1270 CTGCAG C^T[GA][CT]AG
SspI 605 AATATT AAT^ATT
StyI 316 CCTTGG C^C[AT][AT]GG
Tsp45I 197 GTGAC ^GT[GC]AC
Tth111I 412 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 683 CCATCCGTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 307 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 418 GTCTAC GT^[AC][GT]AC
1114 GTCTAC
AciI 835 CCGC CCGC(-3/-1)
445 GCGG
BbvI 1272 GCAGC GCAGC(8/12)
1269 GCTGC
BfaI 385 CTAG C^TAG
851 CTAG
BsaJI 316 CCTTGG C^C[ACGT][ACGT]GG
518 CCTGGG
BseRI 505 GAGGAG GAGGAG(10/8)
904 GAGGAG
BsmFI 147 GTCCC GGGAC(10/14)
832 GTCCC
BspMI 808 ACCTGC ACCTGC(4/8)
1075 ACCTGC
BsrI 921 ACTGG ACTGG(1/-1)
1253 CCAGT
BstF5I 397 CATCC GGATG(2/0)
684 CATCC
Cac8I 565 GCTTGC GC[ACGT]^[ACGT]GC
994 GCTGGC
EarI 47 GAAGAG CTCTTC(1/4)
502 GAAGAG
EcoNI 35 CCTTAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1302 CCTTGCTAAGG
Fnu4HI 1269 GCTGC GC^[ACGT]GC
1272 GCAGC
FokI 397 CATCC GGATG(9/13)
684 CATCC
ItaI 1269 GCTGC GC^[ACGT]GC
1272 GCAGC
Ksp632I 47 GAAGAG CTCTTC(1/4)
502 GAAGAG
MaeI 385 CTAG C^TAG
851 CTAG
MaeII 429 ACGT A^CGT
928 ACGT
MfeI 214 CAATTG C^AATTG
637 CAATTG
MunI 214 CAATTG C^AATTG
637 CAATTG
PleI 145 GAGTC GAGTC(4/5)
983 GAGTC
TfiI 129 GAATC G^A[AT]TC
267 GAATC
TseI 1269 GCTGC G^C[AT]GC
1272 GCAGC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
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AcsI AluI AlwI ApoI BsiYI
BslI BsmAI BssKI BstNI BstYI
DdeI DpnII EcoRII HinfI MaeIII
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NspI Sau3AI
ScrFI SfaNI TaqI Tru9I Tsp509I
TspRI TthHB8I XhoII