Restriction map for
pFN21AE1909
(Circular) (Six-base) MAPSORT of: pFN21AE1909.seq Check: 3263 from: 1 to: 6037
pFN21AE1909 6037 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3120 3380 1937
Size: 487 696 260 4594
Fragments arranged by size:
4594 696 487 260
Acc65I G'GTAC_C
Cuts at: 3358 3358
Size: 6037
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2526 2889 4100 5484 5752 1632
Size: 632 262 363 1211 1384 268 1917
Fragments arranged by size:
1917 1384 1211 632 363 268 262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AlwNI CAG_nnn'CTG
Cuts at: 3235 3780 5352 3235
Size: 545 1572 3920
Fragments arranged by size:
3920 1572 545
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 2180 2498 3167 3346 3572 3724 5833 1493
Size: 687 318 669 179 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 669 318 226 179 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 3362 1870
Size: 73 1419 4545
Fragments arranged by size:
4545 1419 73
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3358 4813 618
Size: 325 217 627 1571 1455 1842
Fragments arranged by size:
1842 1571 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2547 3356 1391
Size: 1156 809 4072
Fragments arranged by size:
4072 1156 809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 928
Size: 971 120 4946
Fragments arranged by size:
4946 971 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4648 136
Size: 107 122 71 631 605 2976 1525
Fragments arranged by size:
2976 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 4098 5254 1790
Size: 2308 1156 2573
Fragments arranged by size:
2573 2308 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3768 4726 442
Size: 416 53 83 399 24 2351 958 1753
Fragments arranged by size:
2351 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2600 2078
Size: 522 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4697 1239
Size: 3458 2579
Bpu10I CC'TnA_GC
Cuts at: 3312 3312
Size: 6037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2472 4700 882
Size: 617 973 2228 2219
Fragments arranged by size:
2228 2219 973 617
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3844 4226 4861 275
Size: 53 83 186 936 2311 382 635 1451
Fragments arranged by size:
2311 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2924 1186
Size: 1339 399 4299
Fragments arranged by size:
4299 1339 399
BseYI C'CCAG_C
Cuts at: 5240 5736 5240
Size: 496 5541
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2743 1163
Size: 573 1007 4457
Fragments arranged by size:
4457 1007 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4248 5276 1540
Size: 275 186 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2547 4098 4183 5254 2547
Size: 1551 85 1071 3330
Fragments arranged by size:
3330 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 3356 4098 4183 5254 1391
Size: 399 757 809 742 85 1071 2174
Fragments arranged by size:
2174 1071 809 757 742 399 85
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 3926 5656 3926
Size: 1730 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 3089 3392 844
Size: 1978 267 303 3489
Fragments arranged by size:
3489 1978 303 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4532 4706 65
Size: 1183 3284 174 1396
Fragments arranged by size:
3284 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4681 4868 1700
Size: 110 2871 187 2869
Fragments arranged by size:
2871 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6037
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6037
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 2882
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2381 2785 3231 3367 3734 4119 4136 5588
Size: 409 404 446 136 367 385 17 1452
Cuts at: 5588 5674 5686 6032 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 446 409 404 385 367 346
136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5298
Fragments arranged by size:
5298 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 3553 4347 4367 2755
Size: 798 794 20 4425
Fragments arranged by size:
4425 798 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 2667 3341 2667
Size: 674 5363
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3484 4373 6002
Size: 54 1425 50 275 18 1654 889 1629
Cuts at: 6002 8
Size: 43
Fragments arranged by size:
1654 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2502 3967 1353
Size: 694 455 1465 3423
Fragments arranged by size:
3423 1465 694 455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 2545
Size: 6037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2526 2889 4100 4697 5484 5752
Size: 393 632 262 363 1211 597 787 268
Cuts at: 5752 1239
Size: 1524
Fragments arranged by size:
1524 1211 787 632 597 393 363 268
262
EcoNI CCTnn'n_nnAGG
Cuts at: 2044 3311 2044
Size: 1267 4770
EcoRI G'AATT_C
Cuts at: 2498 3346 3724 2498
Size: 848 378 4811
Fragments arranged by size:
4811 848 378
EcoRV GAT'ATC
Cuts at: 2621 2621
Size: 6037
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 2904 5184 1678
Size: 1226 2280 2531
Fragments arranged by size:
2531 2280 1226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 4678 4710 4752
Size: 32 135 32 1142 32 1932 32 42
Cuts at: 4752 4784 1373
Size: 32 2626
Fragments arranged by size:
2626 1932 1142 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 3381 3627 669
Size: 988 281 259 1184 246 3079
Fragments arranged by size:
3079 1184 988 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 3362 3362
Size: 6037
MfeI C'AATT_G
Cuts at: 2219 2642 3636 2219
Size: 423 994 4620
Fragments arranged by size:
4620 994 423
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 2682 5151 5335 1305
Size: 763 614 2469 184 2007
Fragments arranged by size:
2469 2007 763 614 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4215
Fragments arranged by size:
4215 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 3995 4354 4513 518
Size: 648 1090 1739 359 159 2042
Fragments arranged by size:
2042 1739 1090 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3453 4130 5278 5523 1606
Size: 1847 677 1148 245 2120
Fragments arranged by size:
2120 1847 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5485
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2132 3035 3050 3395 4940 1132
Size: 97 903 903 15 345 1545 2229
Fragments arranged by size:
2229 1545 903 903 345 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6037
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2796 4106 4138 5619 5651 2764
Size: 32 1310 32 1481 32 3150
Fragments arranged by size:
3150 1481 1310 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 2099 3607 2099
Size: 1508 4529
PstI C_TGCA'G
Cuts at: 830 3279 3389 830
Size: 2449 110 3478
Fragments arranged by size:
3478 2449 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
SacI G_AGCT'C
Cuts at: 2547 2547
Size: 6037
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SapI GCTCTTCn'nnn_
Cuts at: 2047 2047
Size: 6037
SbfI CC_TGCA'GG
Cuts at: 3389 3389
Size: 6037
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 3275 3385 4520 5201 5392 826
Size: 219 2230 110 1135 681 191 1471
Fragments arranged by size:
2230 1471 1135 681 219 191 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 3364 1872
Size: 1492 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 2612 3961 5
Size: 47 2560 1349 2081
Fragments arranged by size:
2560 2081 1349 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6037
StyI C'CwwG_G
Cuts at: 513 1065 2321 3476 513
Size: 552 1256 1155 3074
Fragments arranged by size:
3074 1256 1155 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 4081 4234 4419 4864 325
Size: 1255 2484 17 153 185 445 1498
Fragments arranged by size:
2484 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4283 5995 96
Size: 274 80 33 51 1443 2306 1712 138
Fragments arranged by size:
2306 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 2420 1316
Size: 1104 4933
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2695 2695
Size: 6037
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 3362 1870
Size: 1492 4545
XmnI GAAnn'nnTTC
Cuts at: 2316 4166 2316
Size: 1850 4187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI