################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MslI MunI NaeI NarI NciI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 630 GAATTC [GA]^AATT[CT] ApaI 1118 GGGCCC GGGCC^C ApoI 630 GAATTC [GA]^AATT[CT] Asp700I 126 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 445 GAGCTC G[AT]GC[AT]^C BamHI 376 GGATCC G^GATCC BsgI 1132 GTGCAG GTGCAG(16/14) BsiEI 41 CGGCCG CG[GA][CT]^CG BsiHKAI 445 GAGCTC G[AT]GC[AT]^C BsrFI 1128 GCCGGT [GA]^CCGG[CT] BstEII 1239 GGTCACC G^GT[ACGT]ACC BstPI 1239 GGTCACC G^GT[ACGT]ACC BstYI 376 GGATCC [GA]^GATC[CT] Cfr10I 1128 GCCGGT [GA]^CCGG[CT] DrdI 1231 GACCCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 41 CGGCCG C^GGCCG EclXI 41 CGGCCG C^GGCCG Eco52I 41 CGGCCG C^GGCCG EcoO65I 1239 GGTCACC G^GT[ACGT]ACC EcoRI 630 GAATTC G^AATTC EcoRV 262 GATATC GAT^ATC FauI 34 CCCGC CCCGC(4/6) HaeII 16 AGCGCC [GA]GCGC^[CT] HapII 1129 CCGG C^CGG HpaII 1129 CCGG C^CGG KspI 35 CCGCGG CCGC^GG MaeII 1112 ACGT A^CGT MflI 376 GGATCC [GA]^GATC[CT] MspI 1129 CCGG C^CGG NcoI 1289 CCATGG C^CATGG NotI 40 GCGGCCGC GC^GGCCGC PpuMI 1229 AGGACCC [GA]G^G[AT]CC[CT] PshAI 877 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 182 CTGCAG CTGCA^G PvuII 1201 CAGCTG CAG^CTG SacI 445 GAGCTC GAGCT^C SacII 35 CCGCGG CCGC^GG SapI 48 GCTCTTC GCTCTTC(1/4) SexAI 827 ACCTGGT A^CC[AT]GGT XbaI 624 TCTAGA T^CTAGA XhoII 376 GGATCC [GA]^GATC[CT] XmnI 126 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### BalI 390 TGGCCA TGG^CCA 1179 TGGCCA BcgI 556 CGACGGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 965 CGAACAGCATGC BglI 19 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 31 GCCCCCGCGGC BpmI 810 CTGGAG CTGGAG(16/14) 998 CTGGAG BsaI 203 GAGACC GGTCTC(1/5) 1011 GGTCTC BseRI 167 GAGGAG GAGGAG(10/8) 200 GAGGAG BsrDI 988 CATTGC GCAATG(2/0) 640 GCAATG BstAPI 493 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1134 GCAGGCTCTGC BstF5I 306 CATCC GGATG(2/0) 817 GGATG DpnII 159 GATC ^GATC 377 GATC DraII 1118 GGGCCCC [GA]G^G[ACGT]CC[CT] 1229 AGGACCC DsaI 35 CCGCGG C^C[GA][CT]GG 1289 CCATGG EarI 49 CTCTTC CTCTTC(1/4) 578 CTCTTC EcoO109I 1118 GGGCCCC [GA]G^G[ACGT]CC[CT] 1229 AGGACCC FokI 306 CATCC GGATG(9/13) 817 GGATG HphI 1212 TCACC GGTGA(8/7) 1241 TCACC Ksp632I 49 CTCTTC CTCTTC(1/4) 578 CTCTTC MboI 159 GATC ^GATC 377 GATC MluNI 390 TGGCCA TGG^CCA 1179 TGGCCA MscI 390 TGGCCA TGG^CCA 1179 TGGCCA MseI 679 TTAA T^TAA 994 TTAA MspA1I 35 CCGCGG C[AC]G^C[GT]G 1201 CAGCTG NdeII 159 GATC ^GATC 377 GATC PleI 576 GACTC GAGTC(4/5) 1157 GACTC Sau3AI 159 GATC ^GATC 377 GATC SfcI 182 CTGCAG C^T[GA][CT]AG 503 CTACAG SphI 489 GCATGC GCATG^C 971 GCATGC TaqI 417 TCGA T^CGA 1265 TCGA TfiI 937 GATTC G^A[AT]TC 1276 GAATC Tru9I 679 TTAA T^TAA 994 TTAA TthHB8I 417 TCGA T^CGA 1265 TCGA XcmI 305 CCATCCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 525 CCAGTGGGGGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AvaII BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MaeI MaeIII MboII MnlI MvaI MvnI MwoI NlaIII NlaIV NspI PflMI Sau96I ScrFI SfaNI StyI TseI Tsp45I Tsp509I TspRI Van91I