(Circular) (Six-base) MAPSORT of: pFN21AE4091.seq Check: 1680 from: 1 to: 6079 pFN21AE4091 6079 bp With 182 enzymes: SgfI * March 28, 2012 13:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3129 4142 5526 5794 1632 Size: 1497 1013 1384 268 1917 Fragments arranged by size: 1917 1497 1384 1013 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 2128 2504 2852 3145 3822 5394 2128 Size: 376 348 293 677 1572 2813 Fragments arranged by size: 2813 1572 677 376 348 293 ApaI G_GGCC'C Cuts at: 3127 3127 Size: 6079 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 2635 3388 3614 3766 5875 1493 Size: 1142 753 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 753 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 BamHI G'GATC_C Cuts at: 2381 3409 3776 2381 Size: 1028 367 4684 Fragments arranged by size: 4684 1028 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2454 3127 3398 1391 Size: 628 435 673 271 4072 Fragments arranged by size: 4072 673 628 435 271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2550 2584 2959 2993 4270 4304 1903 Size: 34 613 34 375 34 1277 34 3678 Fragments arranged by size: 3678 1277 613 375 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 2042 4690 Size: 107 122 71 631 605 358 12 2648 Cuts at: 4690 136 Size: 1525 Fragments arranged by size: 2648 1525 631 605 358 122 107 71 12 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2573 2766 3127 4140 5296 1790 Size: 783 193 361 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 783 361 193 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 3810 4768 Size: 416 53 83 399 24 838 1555 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1753 1555 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2228 2228 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2836 3024 4739 1239 Size: 1597 188 1715 2579 Fragments arranged by size: 2579 1715 1597 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2202 3022 4742 882 Size: 617 703 820 1720 2219 Fragments arranged by size: 2219 1720 820 703 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2187 2220 1186 Size: 1001 33 5045 Fragments arranged by size: 5045 1001 33 BseYI C'CCAG_C Cuts at: 2018 2121 2331 5282 5778 2018 Size: 103 210 2951 496 2319 Fragments arranged by size: 2951 2319 496 210 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3158 1163 Size: 573 1422 4084 Fragments arranged by size: 4084 1422 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3529 4290 5318 1540 Size: 275 186 48 1480 761 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2454 4140 4225 5296 2454 Size: 1686 85 1071 3237 Fragments arranged by size: 3237 1686 1071 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2454 2573 2766 3127 3398 4140 Size: 399 229 435 119 193 361 271 742 Cuts at: 4140 4225 5296 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 361 271 229 193 119 85 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3434 844 Size: 2590 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2652 2992 4574 4748 65 Size: 1183 1404 340 1582 174 1396 Fragments arranged by size: 1582 1404 1396 1183 340 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3133 4723 4910 1700 Size: 110 1323 1590 187 2869 Fragments arranged by size: 2869 1590 1323 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 2504 3145 794 Size: 1710 641 3728 Fragments arranged by size: 3728 1710 641 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 3244 1184 Size: 2060 4019 BstYI r'GATC_y Cuts at: 1972 2381 3409 3776 4161 4178 5630 5716 5728 Size: 409 1028 367 385 17 1452 86 12 Cuts at: 5728 6074 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1028 409 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 3294 513 Size: 552 939 36 1254 3298 Fragments arranged by size: 3298 1254 939 552 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2805 3595 4389 4409 2805 Size: 790 794 20 4475 Fragments arranged by size: 4475 794 790 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3242 5086 809 Size: 507 1926 1844 1802 Fragments arranged by size: 1926 1844 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2095 2395 Size: 54 1425 50 275 18 216 49 300 Cuts at: 2395 3184 3526 4415 6044 8 Size: 789 342 889 1629 43 Fragments arranged by size: 1629 1425 889 789 342 300 275 216 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3526 1537 Size: 275 234 1480 4090 Fragments arranged by size: 4090 1480 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2589 4009 1353 Size: 707 529 1420 3423 Fragments arranged by size: 3423 1420 707 529 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2452 2452 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2836 3024 3129 4142 4739 5526 5794 Size: 393 1204 188 105 1013 597 787 268 Cuts at: 5794 1239 Size: 1524 Fragments arranged by size: 1524 1204 1013 787 597 393 268 188 105 EcoO109I rG'GnC_Cy Cuts at: 3124 3235 3124 Size: 111 5968 EcoRI G'AATT_C Cuts at: 2635 3388 3766 2635 Size: 753 378 4948 Fragments arranged by size: 4948 753 378 EcoRV GAT'ATC Cuts at: 2269 2269 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2025 5226 1678 Size: 347 3201 2531 Fragments arranged by size: 3201 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3266 3298 4720 4752 4794 Size: 32 135 32 1694 32 1422 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 1694 1422 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3669 669 Size: 988 281 1485 246 3079 Fragments arranged by size: 3079 1485 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2338 2409 2886 5193 5377 1305 Size: 1033 71 477 2307 184 2007 Fragments arranged by size: 2307 2007 1033 477 184 71 MscI TGG'CCA Cuts at: 10 64 1489 1832 2397 3186 10 Size: 54 1425 343 565 789 2903 Fragments arranged by size: 2903 1425 789 565 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4037 4396 4555 518 Size: 648 2871 359 159 2042 Fragments arranged by size: 2871 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 3208 3495 4172 5320 5565 1606 Size: 436 1166 287 677 1148 245 2120 Fragments arranged by size: 2120 1166 1148 677 436 287 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3294 513 Size: 552 939 1290 3298 Fragments arranged by size: 3298 1290 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 2046 3526 2046 Size: 1480 4599 NspI r_CATG'y Cuts at: 1132 1229 2498 2980 3094 3437 4982 1132 Size: 97 1269 482 114 343 1545 2229 Fragments arranged by size: 2229 1545 1269 482 343 114 97 PasI CC'CwG_GG Cuts at: 1348 2573 1348 Size: 1225 4854 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 3120 3193 1108 Size: 1212 800 73 3994 Fragments arranged by size: 3994 1212 800 73 PfoI T'CCnGG_A Cuts at: 3231 3231 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3235 3235 Size: 6079 PshAI GACnn'nnGTC Cuts at: 1613 2886 1613 Size: 1273 4806 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 3123 3123 Size: 6079 PstI C_TGCA'G Cuts at: 830 2191 3431 830 Size: 1361 1240 3478 Fragments arranged by size: 3478 1361 1240 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 3208 3208 Size: 6079 SacI G_AGCT'C Cuts at: 2454 2454 Size: 6079 SacII CC_GC'GG Cuts at: 2043 2043 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 6079 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SexAI A'CCwGG_T Cuts at: 2832 2832 Size: 6079 SfcI C'TryA_G Cuts at: 826 1045 2187 2508 3427 4562 5243 5434 826 Size: 219 1142 321 919 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 919 681 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4183 5084 5346 820 Size: 197 926 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 2498 2980 3437 1132 Size: 1366 482 457 3774 Fragments arranged by size: 3774 1366 482 457 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 3192 3240 3294 3518 513 Size: 552 939 1188 48 54 224 3074 Fragments arranged by size: 3074 1188 939 552 224 54 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4325 6037 96 Size: 274 80 33 51 1443 2348 1712 138 Fragments arranged by size: 2348 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2772 2804 3153 3185 6039 6071 2772 Size: 32 349 32 2854 32 2780 Fragments arranged by size: 2854 2780 349 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 2629 3415 2629 Size: 786 5293 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 2537 2317 Size: 220 5859 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 2135 4208 2135 Size: 2073 4006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI