(Linear) (Six-base) MAPSORT of: KIEE4091.seq Check: 9905 from: 1 to: 1374 KIEE4091.orf With 182 enzymes: SgfI * March 22, 2012 17:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1124 1374 Size: 1124 250 AlwNI CAG_nnn'CTG Cuts at: 0 123 499 847 1140 1374 Size: 123 376 348 293 234 Fragments arranged by size: 376 348 293 234 123 ApaI G_GGCC'C Cuts at: 0 1122 1374 Size: 1122 252 ApoI r'AATT_y Cuts at: 0 630 1374 Size: 630 744 BamHI G'GATC_C Cuts at: 0 376 1374 Size: 376 998 BanII G_rGCy'C Cuts at: 0 14 449 1122 1374 Size: 14 435 673 252 Fragments arranged by size: 673 435 252 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 545 579 954 988 1374 Size: 545 34 375 34 386 Fragments arranged by size: 545 386 375 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 25 37 1374 Size: 25 12 1337 Fragments arranged by size: 1337 25 12 Bme1580I G_kGCm'C Cuts at: 0 568 761 1122 1374 Size: 568 193 361 252 Fragments arranged by size: 568 361 252 193 BmrI ACTGGGnnnn_n' Cuts at: 0 250 1374 Size: 250 1124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 223 1374 Size: 223 1151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 831 1019 1374 Size: 831 188 355 Fragments arranged by size: 831 355 188 BsaI GGTCTCn'nnnn_ Cuts at: 0 197 1017 1374 Size: 197 820 357 Fragments arranged by size: 820 357 197 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 182 215 1374 Size: 182 33 1159 Fragments arranged by size: 1159 182 33 BseYI C'CCAG_C Cuts at: 0 13 116 326 1374 Size: 13 103 210 1048 Fragments arranged by size: 1048 210 103 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1153 1374 Size: 1153 221 BsiEI CG_ry'CG Cuts at: 0 44 1374 Size: 44 1330 BsiHKAI G_wGCw'C Cuts at: 0 449 1374 Size: 449 925 Bsp1286I G_dGCh'C Cuts at: 0 14 449 568 761 1122 1374 Size: 14 435 119 193 361 252 Fragments arranged by size: 435 361 252 193 119 14 BsrDI GCAATG_nn' Cuts at: 0 647 987 1374 Size: 647 340 387 Fragments arranged by size: 647 387 340 BsrFI r'CCGG_y Cuts at: 0 1128 1374 Size: 1128 246 BstAPI GCAn_nnn'nTGC Cuts at: 0 499 1140 1374 Size: 499 641 234 Fragments arranged by size: 641 499 234 BstEII G'GTnAC_C Cuts at: 0 1239 1374 Size: 1239 135 BstYI r'GATC_y Cuts at: 0 376 1374 Size: 376 998 BtgI C'CryG_G Cuts at: 0 35 1289 1374 Size: 35 1254 85 Fragments arranged by size: 1254 85 35 BtsI GCAGTG_nn' Cuts at: 0 800 1374 Size: 800 574 DrdI GACnn_nn'nnGTC Cuts at: 0 1237 1374 Size: 1237 137 EaeI y'GGCC_r Cuts at: 0 41 90 390 1179 1374 Size: 41 49 300 789 195 Fragments arranged by size: 789 300 195 49 41 EagI C'GGCC_G Cuts at: 0 41 1374 Size: 41 1333 EarI CTCTTCn'nnn_ Cuts at: 0 55 584 1374 Size: 55 529 790 Fragments arranged by size: 790 529 55 EcoICRI GAG'CTC Cuts at: 0 447 1374 Size: 447 927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 831 1019 1124 1374 Size: 831 188 105 250 Fragments arranged by size: 831 250 188 105 EcoO109I rG'GnC_Cy Cuts at: 0 1119 1230 1374 Size: 1119 111 144 Fragments arranged by size: 1119 144 111 EcoRI G'AATT_C Cuts at: 0 630 1374 Size: 630 744 EcoRV GAT'ATC Cuts at: 0 264 1374 Size: 264 1110 HaeII r_GCGC'y Cuts at: 0 20 1374 Size: 20 1354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1261 1293 1374 Size: 1261 32 81 Fragments arranged by size: 1261 81 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 333 404 881 1374 Size: 333 71 477 493 Fragments arranged by size: 493 477 333 71 MscI TGG'CCA Cuts at: 0 392 1181 1374 Size: 392 789 193 Fragments arranged by size: 789 392 193 MspA1I CmG'CkG Cuts at: 0 37 1203 1374 Size: 37 1166 171 Fragments arranged by size: 1166 171 37 NcoI C'CATG_G Cuts at: 0 1289 1374 Size: 1289 85 NotI GC'GGCC_GC Cuts at: 0 41 1374 Size: 41 1333 NspI r_CATG'y Cuts at: 0 493 975 1089 1374 Size: 493 482 114 285 Fragments arranged by size: 493 482 285 114 PasI CC'CwG_GG Cuts at: 0 568 1374 Size: 568 806 PflMI CCAn_nnn'nTGG Cuts at: 0 315 1115 1188 1374 Size: 315 800 73 186 Fragments arranged by size: 800 315 186 73 PfoI T'CCnGG_A Cuts at: 0 1226 1374 Size: 1226 148 PpuMI rG'GwC_Cy Cuts at: 0 1230 1374 Size: 1230 144 PshAI GACnn'nnGTC Cuts at: 0 881 1374 Size: 881 493 PspOMI G'GGCC_C Cuts at: 0 1118 1374 Size: 1118 256 PstI C_TGCA'G Cuts at: 0 186 1374 Size: 186 1188 PvuII CAG'CTG Cuts at: 0 1203 1374 Size: 1203 171 SacI G_AGCT'C Cuts at: 0 449 1374 Size: 449 925 SacII CC_GC'GG Cuts at: 0 38 1374 Size: 38 1336 SapI GCTCTTCn'nnn_ Cuts at: 0 55 1374 Size: 55 1319 SexAI A'CCwGG_T Cuts at: 0 827 1374 Size: 827 547 SfcI C'TryA_G Cuts at: 0 182 503 1374 Size: 182 321 871 Fragments arranged by size: 871 321 182 SphI G_CATG'C Cuts at: 0 493 975 1374 Size: 493 482 399 Fragments arranged by size: 493 482 399 StyI C'CwwG_G Cuts at: 0 1187 1235 1289 1374 Size: 1187 48 54 85 Fragments arranged by size: 1187 85 54 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 767 799 1148 1180 1374 Size: 767 32 349 32 194 Fragments arranged by size: 767 349 194 32 32 XbaI T'CTAG_A Cuts at: 0 624 1374 Size: 624 750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 312 532 1374 Size: 312 220 842 Fragments arranged by size: 842 312 220 XmnI GAAnn'nnTTC Cuts at: 0 130 1374 Size: 130 1244 Enzymes that do cut: AcuI AlwNI ApaI ApoI BamHI BanII BcgI BglI Bme1580I BmrI BplI BpmI BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BstEII BstYI BtgI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I MmeI MscI MspA1I NcoI NotI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SexAI SfcI SphI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EciI EcoNI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI