################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboII MluI MluNI MroI MscI MseI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 140 CGCG CG^CG AfaI 266 GTAC GT^AC AflIII 311 ACACGT A^C[GA][CT]GT AluI 194 AGCT AG^CT AspHI 341 GAGCAC G[AT]GC[AT]^C AvaII 45 GGTCC G^G[AT]CC BanII 219 GGGCTC G[GA]GC[CT]^C BcnI 114 CCCGG CC^[GC]GG BglII 241 AGATCT A^GATCT BsaI 383 GAGACC GGTCTC(1/5) BsaJI 92 CCCTGG C^C[ACGT][ACGT]GG BsiHKAI 341 GAGCAC G[AT]GC[AT]^C BsmAI 383 GAGAC GTCTC(1/5) BsmI 414 GAATGC GAATGC(1/-1) BssHII 139 GCGCGC G^CGCGC BstUI 140 CGCG CG^CG BstYI 241 AGATCT [GA]^GATC[CT] Cfr13I 45 GGTCC G^G[ACGT]CC DpnII 242 GATC ^GATC EaeI 116 CGGCCA [CT]^GGCC[GA] EcoNI 48 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 115 CCGG C^CGG HpaII 115 CCGG C^CGG MaeII 313 ACGT A^CGT MboI 242 GATC ^GATC MfeI 329 CAATTG C^AATTG MflI 241 AGATCT [GA]^GATC[CT] MspI 115 CCGG C^CGG MunI 329 CAATTG C^AATTG MvnI 140 CGCG CG^CG NciI 114 CCCGG CC^[GC]GG NdeII 242 GATC ^GATC NlaIV 220 GGCTCC GG[ACGT]^[ACGT]CC RsaI 266 GTAC GT^AC Sau3AI 242 GATC ^GATC Sau96I 45 GGTCC G^G[ACGT]CC ScaI 265 AGTACT AGT^ACT SfaNI 38 GCATC GCATC(5/9) Tsp509I 330 AATT ^AATT XcmI 321 CCAAACTGCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 241 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AciI 249 GCGG CCGC(-3/-1) 254 GCGG BsrI 159 ACTGG ACTGG(1/-1) 188 CCAGT BstXI 188 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 368 CCAGCGACTTGG CfoI 139 GCGC GCG^C 141 GCGC HaeIII 117 GGCC GG^CC 186 GGCC HhaI 139 GCGC GCG^C 141 GCGC HinP1I 139 GCGC G^CGC 141 GCGC NspI 272 GCATGC [GA]CATG^[CT] 404 GCATGC SphI 272 GCATGC GCATG^C 404 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### BbvI BmyI BpmI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I DdeI EcoRII Fnu4HI ItaI MnlI MvaI MwoI NlaIII ScrFI TseI TspRI