################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsiWI BsmBI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MunI NarI NcoI NdeI NheI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SapI SbfI SfiI SfoI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1660 GACGTC GACGT^C AccI 1411 GTCTAC GT^[AC][GT]AC AcsI 1975 GAATTC [GA]^AATT[CT] AcyI 1660 GACGTC G[GA]^CG[CT]C AocI 1366 CCTGAGG CC^T[ACGT]AGG ApaI 318 GGGCCC GGGCC^C ApoI 1975 GAATTC [GA]^AATT[CT] AvaI 1301 CCCGGG C^[CT]CG[GA]G BalI 491 TGGCCA TGG^CCA BanI 1443 GGTGCC G^G[CT][GA]CC BbsI 643 GAAGAC GAAGAC(2/6) BciVI 585 GGATAC GTATCC(6/5) BglI 331 GCCAGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 643 GAAGAC GAAGAC(2/6) BsaHI 1660 GACGTC G[GA]^CG[CT]C BsmI 2245 GCATTC GAATGC(1/-1) BsoBI 1301 CCCGGG C^[CT]CG[GA]G BspHI 888 TCATGA T^CATGA BsrBI 2136 GAGCGG CCGCTC(-3/-3) BstBI 2213 TTCGAA TT^CGAA BstEII 423 GGTCACC G^GT[ACGT]ACC BstPI 423 GGTCACC G^GT[ACGT]ACC Bsu36I 1366 CCTGAGG CC^T[ACGT]AGG DraIII 1810 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1366 CCTGAGG CC^T[ACGT]AGG EcoO65I 423 GGTCACC G^GT[ACGT]ACC EcoRI 1975 GAATTC G^AATTC Hin1I 1660 GACGTC G[GA]^CG[CT]C MluNI 491 TGGCCA TGG^CCA MscI 491 TGGCCA TGG^CCA NotI 985 GCGGCCGC GC^GGCCGC NspV 2213 TTCGAA TT^CGAA PpuMI 2193 AGGACCT [GA]G^G[AT]CC[CT] RcaI 888 TCATGA T^CATGA SacI 2272 GAGCTC GAGCT^C SexAI 1811 ACCTGGT A^CC[AT]GGT SfuI 2213 TTCGAA TT^CGAA SgrAI 850 CACCGGCG C[GA]^CCGG[CT]G SmaI 1301 CCCGGG CCC^GGG SspI 1952 AATATT AAT^ATT XmaCI 1301 CCCGGG C^CCGGG XmaI 1301 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 597 TCCGGA T^CCGGA 2237 TCCGGA AflIII 1631 ACATGT A^C[GA][CT]GT 1709 ACATGT AlwNI 277 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 562 CAGTACCTG Asp700I 252 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1509 GAACATCTTC BlpI 2088 GCTGAGC GC^T[ACGT]AGC 2274 GCTCAGC Bpu1102I 2088 GCTGAGC GC^T[ACGT]AGC 2274 GCTCAGC BseAI 597 TCCGGA T^CCGGA 2237 TCCGGA BsgI 590 CTGCAC GTGCAG(16/14) 1111 CTGCAC BspEI 597 TCCGGA T^CCGGA 2237 TCCGGA BspLU11I 1631 ACATGT A^CATGT 1709 ACATGT BspMI 2095 ACCTGC ACCTGC(4/8) 1095 GCAGGT BstAPI 394 GCACCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1008 GCATCTGCTGC BstXI 482 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1718 CCAACATCATGG BstYI 1904 AGATCC [GA]^GATC[CT] 2234 AGATCC CelII 2088 GCTGAGC GC^T[ACGT]AGC 2274 GCTCAGC EcoT14I 188 CCAAGG C^C[AT][AT]GG 494 CCAAGG MflI 1904 AGATCC [GA]^GATC[CT] 2234 AGATCC MroI 597 TCCGGA T^CCGGA 2237 TCCGGA MseI 898 TTAA T^TAA 1577 TTAA NaeI 1163 GCCGGC GCC^GGC 1795 GCCGGC NgoMIV 1163 GCCGGC G^CCGGC 1795 GCCGGC PstI 202 CTGCAG CTGCA^G 2131 CTGCAG ScaI 1271 AGTACT AGT^ACT 1328 AGTACT SmlI 1861 CTCAAG C^T[CT][GA]AG 2075 CTCAAG StyI 188 CCAAGG C^C[AT][AT]GG 494 CCAAGG TfiI 267 GATTC G^A[AT]TC 871 GAATC Tru9I 898 TTAA T^TAA 1577 TTAA Tsp509I 1976 AATT ^AATT 2179 AATT XhoII 1904 AGATCC [GA]^GATC[CT] 2234 AGATCC XmnI 252 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1509 GAACATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI