(Circular) (Six-base) MAPSORT of: pFN21AE6740.seq Check: 9084 from: 1 to: 7033 pFN21AE6740 7033 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3091 4109 3091 Size: 1018 6015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3669 4843 278 Size: 53 83 186 936 2133 1174 2468 Fragments arranged by size: 2468 2133 1174 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3417 4376 1937 Size: 1480 959 4594 Fragments arranged by size: 4594 1480 959 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 4332 5160 5533 1563 Size: 2769 828 373 3063 Fragments arranged by size: 3063 2769 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 4224 5096 6480 6748 1632 Size: 453 2139 872 1384 268 1917 Fragments arranged by size: 2139 1917 1384 872 453 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 3636 3714 5932 6909 1203 Size: 2433 78 2218 977 1327 Fragments arranged by size: 2433 2218 1327 977 78 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2867 2899 2867 Size: 32 7001 AlwNI CAG_nnn'CTG Cuts at: 2287 2572 4776 6348 2287 Size: 285 2204 1572 2972 Fragments arranged by size: 2972 2204 1572 285 ApaI G_GGCC'C Cuts at: 2327 2327 Size: 7033 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 3980 4342 4568 4720 6829 1493 Size: 2487 362 226 152 2109 1697 Fragments arranged by size: 2487 2109 1697 362 226 152 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 3306 4358 1870 Size: 73 1363 1052 4545 Fragments arranged by size: 4545 1363 1052 73 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4354 5809 618 Size: 325 217 627 1661 906 1455 1842 Fragments arranged by size: 1842 1661 1455 906 627 325 217 BanII G_rGCy'C Cuts at: 1391 2327 3656 4281 4352 1391 Size: 936 1329 625 71 4072 Fragments arranged by size: 4072 1329 936 625 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2655 928 Size: 971 756 5306 Fragments arranged by size: 5306 971 756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2584 4925 6135 2584 Size: 2341 1210 3482 Fragments arranged by size: 3482 2341 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7033 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2342 5644 136 Size: 107 122 71 631 605 670 3302 1525 Fragments arranged by size: 3302 1525 670 631 605 122 107 71 BglII A'GATC_T Cuts at: 7028 7028 Size: 7033 BlpI GC'TnA_GC Cuts at: 4094 4280 4450 4094 Size: 186 170 6677 Fragments arranged by size: 6677 186 170 Bme1580I G_kGCm'C Cuts at: 1790 2327 3453 5094 6250 1790 Size: 537 1126 1641 1156 2573 Fragments arranged by size: 2573 1641 1156 1126 537 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 2560 4764 Size: 416 53 83 399 24 858 285 2204 Cuts at: 4764 5722 442 Size: 958 1753 Fragments arranged by size: 2204 1753 958 858 416 399 285 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2394 2765 2804 3335 3530 5693 1239 Size: 1155 371 39 531 195 2163 2579 Fragments arranged by size: 2579 2163 1155 531 371 195 39 Bpu10I CC'TnA_GC Cuts at: 3764 3764 Size: 7033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3851 4065 5158 6023 6321 3851 Size: 214 1093 865 298 4563 Fragments arranged by size: 4563 1093 865 298 214 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 2780 4256 5696 882 Size: 617 735 546 1476 1440 2219 Fragments arranged by size: 2219 1476 1440 735 617 546 BsaAI yAC'GTr Cuts at: 493 1204 4824 493 Size: 711 3620 2702 Fragments arranged by size: 3620 2702 711 BsaBI GATnn'nnATC Cuts at: 7027 7027 Size: 7033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3666 4840 5222 5857 Size: 53 83 186 936 2133 1174 382 635 Cuts at: 5857 275 Size: 1451 Fragments arranged by size: 2133 1451 1174 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2602 3877 4242 5466 5864 6138 6285 6696 2602 Size: 1275 365 1224 398 274 147 411 2939 Fragments arranged by size: 2939 1275 1224 411 398 365 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2584 2614 3311 Size: 30 165 30 684 30 467 30 697 Cuts at: 3311 3341 1178 Size: 30 4870 Fragments arranged by size: 4870 697 684 467 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2622 3285 3332 3389 3854 3918 1186 Size: 1436 663 47 57 465 64 4301 Fragments arranged by size: 4301 1436 663 465 64 57 47 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2580 3101 1163 Size: 573 844 521 5095 Fragments arranged by size: 5095 844 573 521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2994 3364 3785 4483 5244 6272 Size: 275 186 993 370 421 698 761 1028 Cuts at: 6272 1540 Size: 2301 Fragments arranged by size: 2301 1028 993 761 698 421 370 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2751 3738 3770 4100 4281 5094 5179 6250 Size: 633 987 32 330 181 813 85 1071 Cuts at: 6250 2118 Size: 2901 Fragments arranged by size: 2901 1071 987 813 633 330 181 85 32 BsmI GAATG_Cn' Cuts at: 4250 4544 4637 4250 Size: 294 93 6646 Fragments arranged by size: 6646 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2327 2751 3453 3656 3738 3770 Size: 399 328 209 424 702 203 82 32 Cuts at: 3770 4100 4281 4352 5094 5179 6250 1391 Size: 330 181 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 424 399 330 328 209 203 181 85 82 71 32 BspEI T'CCGG_A Cuts at: 2602 4242 6696 2602 Size: 1640 2454 2939 Fragments arranged by size: 2939 2454 1640 BspHI T'CATG_A Cuts at: 2893 4922 6652 2893 Size: 2029 1730 3274 Fragments arranged by size: 3274 2029 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3091 4109 4388 844 Size: 2247 1018 279 3489 Fragments arranged by size: 3489 2247 1018 279 BsrBI CCG'CTC Cuts at: 1394 1823 4143 4920 1394 Size: 429 2320 777 3507 Fragments arranged by size: 3507 2320 777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3915 4071 4279 5528 5702 65 Size: 1183 2667 156 208 1249 174 1396 Fragments arranged by size: 2667 1396 1249 1183 208 174 156 BsrFI r'CCGG_y Cuts at: 1700 1810 2856 3168 3800 5677 5864 1700 Size: 110 1046 312 632 1877 187 2869 Fragments arranged by size: 2869 1877 1046 632 312 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 5093 6105 5093 Size: 1012 6021 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 3019 794 Size: 1611 614 4808 Fragments arranged by size: 4808 1611 614 BstBI TT'CG_AA Cuts at: 4219 5837 4219 Size: 1618 5415 BstEII G'GTnAC_C Cuts at: 1184 2428 1184 Size: 1244 5789 BstXI CCAn_nnnn'nTGG Cuts at: 2494 3730 2494 Size: 1236 5797 BstYI r'GATC_y Cuts at: 1972 3909 4239 4363 4730 5115 5132 6584 6670 Size: 1937 330 124 367 385 17 1452 86 Cuts at: 6670 6682 7028 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1937 1452 385 367 346 330 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3372 3372 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 2552 2582 3344 3853 513 Size: 552 939 32 516 30 762 509 3693 Fragments arranged by size: 3693 939 762 552 516 509 32 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 2636 3834 4549 5343 5363 2636 Size: 1198 715 794 20 4306 Fragments arranged by size: 4306 1198 794 715 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2346 2381 529 Size: 35 1782 35 5181 Fragments arranged by size: 5181 1782 35 35 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DraIII CAC_nnn'GTG Cuts at: 3820 3820 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 2496 2991 Size: 54 1425 50 275 18 513 153 495 Cuts at: 2991 3361 3782 4480 5369 6998 8 Size: 370 421 698 889 1629 43 Fragments arranged by size: 1629 1425 889 698 513 495 421 370 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2991 3361 3782 4480 1537 Size: 275 1179 370 421 698 4090 Fragments arranged by size: 4090 1179 698 421 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2601 2927 3581 3866 4305 4963 1353 Size: 765 483 326 654 285 439 658 3423 Fragments arranged by size: 3423 765 658 654 483 439 326 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 2603 5620 5994 6140 387 Size: 1064 138 519 495 3017 374 146 1280 Fragments arranged by size: 3017 1280 1064 519 495 374 146 138 EcoICRI GAG'CTC Cuts at: 4279 4279 Size: 7033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2394 2765 2804 3335 3530 4224 Size: 393 453 309 371 39 531 195 694 Cuts at: 4224 5096 5693 6480 6748 1239 Size: 872 597 787 268 1524 Fragments arranged by size: 1524 872 787 694 597 531 453 393 371 309 268 195 39 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3533 3989 3098 Size: 435 456 6142 Fragments arranged by size: 6142 456 435 EcoO109I rG'GnC_Cy Cuts at: 2323 2324 2503 2611 4199 2323 Size: 1 179 108 1588 5157 Fragments arranged by size: 5157 1588 179 108 1 EcoRI G'AATT_C Cuts at: 3980 4342 4720 3980 Size: 362 378 6293 Fragments arranged by size: 6293 378 362 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2630 2630 Size: 7033 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2216 2248 3602 Size: 32 135 32 557 32 55 32 1354 Cuts at: 3602 3634 5674 5706 5748 5780 1373 Size: 32 2040 32 42 32 2626 Fragments arranged by size: 2626 2040 1354 557 135 55 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4377 4623 669 Size: 988 281 2439 246 3079 Fragments arranged by size: 3079 2439 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3669 6147 6331 1305 Size: 2364 2478 184 2007 Fragments arranged by size: 2478 2364 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 4545 Fragments arranged by size: 4545 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2985 3446 3731 3920 4991 5350 5509 Size: 648 1819 461 285 189 1071 359 159 Cuts at: 5509 518 Size: 2042 Fragments arranged by size: 2042 1819 1071 648 461 359 285 189 159 MspA1I CmG'CkG Cuts at: 1606 2027 2038 2425 2439 2554 2584 4449 5126 Size: 421 11 387 14 115 30 1865 677 Cuts at: 5126 6274 6519 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1865 1148 677 421 387 245 115 30 14 11 NaeI GCC'GGC Cuts at: 3170 3802 3170 Size: 632 6401 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5542 Fragments arranged by size: 5542 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NgoMIV G'CCGG_C Cuts at: 3168 3800 3168 Size: 632 6401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 2991 4480 2991 Size: 1489 5544 NspI r_CATG'y Cuts at: 1132 1229 2704 3433 3640 3718 4004 4391 5936 Size: 97 1475 729 207 78 286 387 1545 Cuts at: 5936 1132 Size: 2229 Fragments arranged by size: 2229 1545 1475 729 387 286 207 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 3636 3714 5932 3636 Size: 78 2218 4737 Fragments arranged by size: 4737 2218 78 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 2815 3497 1108 Size: 1464 243 682 4644 Fragments arranged by size: 4644 1464 682 243 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4199 4199 Size: 7033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 4191 4603 4191 Size: 412 6621 PspOMI G'GGCC_C Cuts at: 2323 2323 Size: 7033 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3677 3709 3677 Size: 32 7001 PstI C_TGCA'G Cuts at: 830 2211 4140 4385 830 Size: 1381 1929 245 3478 Fragments arranged by size: 3478 1929 1381 245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 SacI G_AGCT'C Cuts at: 4281 4281 Size: 7033 SacII CC_GC'GG Cuts at: 2039 2555 2585 2039 Size: 516 30 6487 Fragments arranged by size: 6487 516 30 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 3278 3335 5281 6993 3278 Size: 57 1946 1712 3318 Fragments arranged by size: 3318 1946 1712 57 SexAI A'CCwGG_T Cuts at: 3816 3816 Size: 7033 SfcI C'TryA_G Cuts at: 826 1045 2207 3694 4136 4381 5516 6197 6388 Size: 219 1162 1487 442 245 1135 681 191 Cuts at: 6388 826 Size: 1471 Fragments arranged by size: 1487 1471 1162 1135 681 442 245 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 2856 1700 Size: 1156 5877 SmaI CCC'GGG Cuts at: 1872 3308 4360 1872 Size: 1436 1052 4545 Fragments arranged by size: 4545 1436 1052 SmlI C'TyrA_G Cuts at: 820 1017 1943 3866 4080 5137 6038 6300 820 Size: 197 926 1923 214 1057 901 262 1553 Fragments arranged by size: 1923 1553 1057 926 901 262 214 197 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 3959 4957 5 Size: 47 3907 998 2081 Fragments arranged by size: 3907 2081 998 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2004 2193 2499 4472 513 Size: 552 939 189 306 1973 3074 Fragments arranged by size: 3074 1973 939 552 306 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2265 3827 5060 5077 5230 5415 5860 Size: 1255 685 1562 1233 17 153 185 445 Cuts at: 5860 325 Size: 1498 Fragments arranged by size: 1562 1498 1255 1233 685 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3276 3333 3708 Size: 274 80 33 51 1443 1299 57 375 Cuts at: 3708 5279 6991 96 Size: 1571 1712 138 Fragments arranged by size: 1712 1571 1443 1299 375 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3141 3173 3626 3658 6993 7025 3141 Size: 32 453 32 3335 32 3149 Fragments arranged by size: 3335 3149 453 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2313 2362 3814 2313 Size: 49 1452 5532 Fragments arranged by size: 5532 1452 49 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 3306 4358 1870 Size: 1436 1052 4545 Fragments arranged by size: 4545 1436 1052 XmnI GAAnn'nnTTC Cuts at: 2261 3518 5162 2261 Size: 1257 1644 4132 Fragments arranged by size: 4132 1644 1257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3667 4841 276 Size: 53 83 186 936 2133 1174 2468 Fragments arranged by size: 2468 2133 1174 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI