(Linear) (Six-base) MAPSORT of: KIEE6740.seq Check: 8146 from: 1 to: 2328 KIEE6740.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1086 2104 2328 Size: 1086 1018 224 Fragments arranged by size: 1086 1018 224 AatII G_ACGT'C Cuts at: 0 1664 2328 Size: 1664 664 AccI GT'mk_AC Cuts at: 0 1412 2328 Size: 1412 916 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 2219 2328 Size: 80 2139 109 Fragments arranged by size: 2139 109 80 AflIII A'CryG_T Cuts at: 0 1631 1709 2328 Size: 1631 78 619 Fragments arranged by size: 1631 619 78 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 862 894 2328 Size: 862 32 1434 Fragments arranged by size: 1434 862 32 AlwNI CAG_nnn'CTG Cuts at: 0 282 567 2328 Size: 282 285 1761 Fragments arranged by size: 1761 285 282 ApaI G_GGCC'C Cuts at: 0 322 2328 Size: 322 2006 ApoI r'AATT_y Cuts at: 0 1975 2328 Size: 1975 353 AvaI C'yCGr_G Cuts at: 0 1301 2328 Size: 1301 1027 BanI G'GyrC_C Cuts at: 0 1443 2328 Size: 1443 885 BanII G_rGCy'C Cuts at: 0 322 1651 2276 2328 Size: 322 1329 625 52 Fragments arranged by size: 1329 625 322 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 650 2328 Size: 650 1678 BciVI GTATCCnnnnn_n' Cuts at: 0 579 2328 Size: 579 1749 BglI GCCn_nnn'nGGC Cuts at: 0 337 2328 Size: 337 1991 BlpI GC'TnA_GC Cuts at: 0 2089 2275 2328 Size: 2089 186 53 Fragments arranged by size: 2089 186 53 Bme1580I G_kGCm'C Cuts at: 0 322 1448 2328 Size: 322 1126 880 Fragments arranged by size: 1126 880 322 BmrI ACTGGGnnnn_n' Cuts at: 0 270 555 2328 Size: 270 285 1773 Fragments arranged by size: 1773 285 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 389 760 799 1330 1525 2328 Size: 389 371 39 531 195 803 Fragments arranged by size: 803 531 389 371 195 39 Bpu10I CC'TnA_GC Cuts at: 0 1759 2328 Size: 1759 569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1846 2060 2328 Size: 1846 214 268 Fragments arranged by size: 1846 268 214 BsaI GGTCTCn'nnnn_ Cuts at: 0 229 775 2251 2328 Size: 229 546 1476 77 Fragments arranged by size: 1476 546 229 77 BsaHI Gr'CG_yC Cuts at: 0 1661 2328 Size: 1661 667 BsaWI w'CCGG_w Cuts at: 0 597 1872 2237 2328 Size: 597 1275 365 91 Fragments arranged by size: 1275 597 365 91 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 82 112 579 609 1306 1336 2328 Size: 82 30 467 30 697 30 992 Fragments arranged by size: 992 697 467 82 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 617 1280 1327 1384 1849 1913 2328 Size: 617 663 47 57 465 64 415 Fragments arranged by size: 663 617 465 415 64 57 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 575 1096 2328 Size: 575 521 1232 Fragments arranged by size: 1232 575 521 BsiEI CG_ry'CG Cuts at: 0 989 1359 1780 2328 Size: 989 370 421 548 Fragments arranged by size: 989 548 421 370 BsiHKAI G_wGCw'C Cuts at: 0 113 746 1733 1765 2095 2276 2328 Size: 113 633 987 32 330 181 52 Fragments arranged by size: 987 633 330 181 113 52 32 BsmI GAATG_Cn' Cuts at: 0 2245 2328 Size: 2245 83 Bsp1286I G_dGCh'C Cuts at: 0 113 322 746 1448 1651 1733 1765 2095 Size: 113 209 424 702 203 82 32 330 Cuts at: 2095 2276 2328 Size: 181 52 Fragments arranged by size: 702 424 330 209 203 181 113 82 52 32 BspEI T'CCGG_A Cuts at: 0 597 2237 2328 Size: 597 1640 91 Fragments arranged by size: 1640 597 91 BspHI T'CATG_A Cuts at: 0 888 2328 Size: 888 1440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1086 2104 2328 Size: 1086 1018 224 Fragments arranged by size: 1086 1018 224 BsrBI CCG'CTC Cuts at: 0 2138 2328 Size: 2138 190 BsrDI GCAATG_nn' Cuts at: 0 1910 2066 2274 2328 Size: 1910 156 208 54 Fragments arranged by size: 1910 208 156 54 BsrFI r'CCGG_y Cuts at: 0 851 1163 1795 2328 Size: 851 312 632 533 Fragments arranged by size: 851 632 533 312 BstAPI GCAn_nnn'nTGC Cuts at: 0 400 1014 2328 Size: 400 614 1314 Fragments arranged by size: 1314 614 400 BstBI TT'CG_AA Cuts at: 0 2214 2328 Size: 2214 114 BstEII G'GTnAC_C Cuts at: 0 423 2328 Size: 423 1905 BstXI CCAn_nnnn'nTGG Cuts at: 0 489 1725 2328 Size: 489 1236 603 Fragments arranged by size: 1236 603 489 BstYI r'GATC_y Cuts at: 0 1904 2234 2328 Size: 1904 330 94 Fragments arranged by size: 1904 330 94 Bsu36I CC'TnA_GG Cuts at: 0 1367 2328 Size: 1367 961 BtgI C'CryG_G Cuts at: 0 31 547 577 1339 1848 2328 Size: 31 516 30 762 509 480 Fragments arranged by size: 762 516 509 480 31 30 BtsI GCAGTG_nn' Cuts at: 0 631 1829 2328 Size: 631 1198 499 Fragments arranged by size: 1198 631 499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 341 376 2328 Size: 341 35 1952 Fragments arranged by size: 1952 341 35 DraIII CAC_nnn'GTG Cuts at: 0 1815 2328 Size: 1815 513 EaeI y'GGCC_r Cuts at: 0 338 491 986 1356 1777 2328 Size: 338 153 495 370 421 551 Fragments arranged by size: 551 495 421 370 338 153 EagI C'GGCC_G Cuts at: 0 986 1356 1777 2328 Size: 986 370 421 551 Fragments arranged by size: 986 551 421 370 EarI CTCTTCn'nnn_ Cuts at: 0 113 596 922 1576 1861 2300 2328 Size: 113 483 326 654 285 439 28 Fragments arranged by size: 654 483 439 326 285 113 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 103 598 2328 Size: 103 495 1730 Fragments arranged by size: 1730 495 103 EcoICRI GAG'CTC Cuts at: 0 2274 2328 Size: 2274 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 389 760 799 1330 1525 2219 2328 Size: 80 309 371 39 531 195 694 109 Fragments arranged by size: 694 531 371 309 195 109 80 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 1093 1528 1984 2328 Size: 1093 435 456 344 Fragments arranged by size: 1093 456 435 344 EcoO109I rG'GnC_Cy Cuts at: 0 318 319 498 606 2194 2328 Size: 318 1 179 108 1588 134 Fragments arranged by size: 1588 318 179 134 108 1 EcoRI G'AATT_C Cuts at: 0 1975 2328 Size: 1975 353 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 625 2328 Size: 625 1703 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 211 243 1597 1629 2328 Size: 124 32 55 32 1354 32 699 Fragments arranged by size: 1354 699 124 55 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1664 2328 Size: 1664 664 MscI TGG'CCA Cuts at: 0 493 2328 Size: 493 1835 MslI CAynn'nnrTG Cuts at: 0 980 1441 1726 1915 2328 Size: 980 461 285 189 413 Fragments arranged by size: 980 461 413 285 189 MspA1I CmG'CkG Cuts at: 0 22 33 420 434 549 579 2328 Size: 22 11 387 14 115 30 1749 Fragments arranged by size: 1749 387 115 30 22 14 11 NaeI GCC'GGC Cuts at: 0 1165 1797 2328 Size: 1165 632 531 Fragments arranged by size: 1165 632 531 NgoMIV G'CCGG_C Cuts at: 0 1163 1795 2328 Size: 1163 632 533 Fragments arranged by size: 1163 632 533 NotI GC'GGCC_GC Cuts at: 0 986 2328 Size: 986 1342 NspI r_CATG'y Cuts at: 0 699 1428 1635 1713 1999 2328 Size: 699 729 207 78 286 329 Fragments arranged by size: 729 699 329 286 207 78 PciI A'CATG_T Cuts at: 0 1631 1709 2328 Size: 1631 78 619 Fragments arranged by size: 1631 619 78 PflMI CCAn_nnn'nTGG Cuts at: 0 567 810 1492 2328 Size: 567 243 682 836 Fragments arranged by size: 836 682 567 243 PfoI T'CCnGG_A Cuts at: 0 509 2328 Size: 509 1819 PpuMI rG'GwC_Cy Cuts at: 0 2194 2328 Size: 2194 134 PsiI TTA'TAA Cuts at: 0 2186 2328 Size: 2186 142 PspOMI G'GGCC_C Cuts at: 0 318 2328 Size: 318 2010 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1672 1704 2328 Size: 1672 32 624 Fragments arranged by size: 1672 624 32 PstI C_TGCA'G Cuts at: 0 206 2135 2328 Size: 206 1929 193 Fragments arranged by size: 1929 206 193 SacI G_AGCT'C Cuts at: 0 2276 2328 Size: 2276 52 SacII CC_GC'GG Cuts at: 0 34 550 580 2328 Size: 34 516 30 1748 Fragments arranged by size: 1748 516 34 30 ScaI AGT'ACT Cuts at: 0 1273 1330 2328 Size: 1273 57 998 Fragments arranged by size: 1273 998 57 SexAI A'CCwGG_T Cuts at: 0 1811 2328 Size: 1811 517 SfcI C'TryA_G Cuts at: 0 202 1689 2131 2328 Size: 202 1487 442 197 Fragments arranged by size: 1487 442 202 197 SgrAI Cr'CCGG_yG Cuts at: 0 851 2328 Size: 851 1477 SmaI CCC'GGG Cuts at: 0 1303 2328 Size: 1303 1025 SmlI C'TyrA_G Cuts at: 0 1861 2075 2328 Size: 1861 214 253 Fragments arranged by size: 1861 253 214 SspI AAT'ATT Cuts at: 0 1954 2328 Size: 1954 374 StyI C'CwwG_G Cuts at: 0 188 494 2328 Size: 188 306 1834 Fragments arranged by size: 1834 306 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 260 1822 2328 Size: 260 1562 506 Fragments arranged by size: 1562 506 260 TatI w'GTAC_w Cuts at: 0 1271 1328 1703 2328 Size: 1271 57 375 625 Fragments arranged by size: 1271 625 375 57 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1136 1168 1621 1653 2328 Size: 1136 32 453 32 675 Fragments arranged by size: 1136 675 453 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 308 357 1809 2328 Size: 308 49 1452 519 Fragments arranged by size: 1452 519 308 49 XmaI C'CCGG_G Cuts at: 0 1301 2328 Size: 1301 1027 XmnI GAAnn'nnTTC Cuts at: 0 256 1513 2328 Size: 256 1257 815 Fragments arranged by size: 1257 815 256 ZraI GAC'GTC Cuts at: 0 1662 2328 Size: 1662 666 Enzymes that do cut: AarI AatII AccI AcuI AflIII AloI AlwNI ApaI ApoI AvaI BanI BanII BbsI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI PciI PflMI PfoI PpuMI PsiI PspOMI PsrI PstI SacI SacII ScaI SexAI SfcI SgrAI SmaI SmlI SspI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BseYI BsiWI BsmBI BsrGI BssHII BssSI BstZ17I BtgZI ClaI DraI DrdI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PspXI PvuI PvuII RsrII SalI SanDI SapI SbfI SfiI SfoI SgfI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI